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The association of FKBP5 polymorphism with asthma susceptibility in asthmatic patients

  • Sura F. Alsaffar ORCID logo EMAIL logo , Haider Abdulhameed Alqaraghuli , Jabbar H. Yenzeel and Haider F. Ghazi ORCID logo
Published/Copyright: June 25, 2021
Journal of Basic and Clinical Physiology and Pharmacology
From the journal Journal of Basic and Clinical Physiology and Pharmacology Volume 32 Issue 4

Abstract

Objectives

Inhaled corticosteroids are the most effective controllers of asthma, although asthmatics vary in their response. FKBP51 is a major component of the glucocorticoid receptor which regulates its responses to corticosteroids. Therefore, the present study aims to identify the role of FKBP5 gene polymorphism in asthma susceptibility and corticosteroid resistance.

Methods

DNA was extracted from the blood of 68 asthmatic and 40 control subjects. FKBP5 gene fragments were amplified by PCR and sequenced by the Sanger method. The sequencing results were aligned by mapping on the reference sequences of National center of Biotechnology Information (NCBI) and single nucleotide polymorphisms (SNPs) which were checked. Finally, the genotype, allele frequency and odds ratio (OR) were calculated.

Results

The FKBP5 fragment sequencing revealed the presence of rs1360780 and one novel SNP found in 17 samples taken from asthmatic patients as compared to db SNP data in the NCBI database. The FKBP5 variant (rs1360780) indicated that the allele frequency of risk allele T was 41.18% in patients and 20% in control group members p<0.001 and OR=2.8 when compared to a wild C allele frequency of 58.82% in patients and 64% in the control group members. The novel SNP FKBP5 was compared to the SNP database in the NCBI database in which wild T allele was substituted with G. The novel SNP was submitted to the ClinVar Submission Portal at NCBI with accession number: rs1581842283 and confirmed an asthma susceptibility risk factor with allele G frequency of 11.76% in asthmatics and 2.5% in the control group members (OR=5.2, p<0.05), as compared to a wild T allele frequency of 88.24% in asthmatics and 97.5% in the control group members.

Conclusions

The risk allele T of rs1360780 and the novel SNP rs1581842283 risk allele G predict asthma susceptibility but show no association with corticosteroid resistant.

Keywords: asthma; corticosteroid resistance; FKBP5 polymorphism
Corresponding author: Sura F. Alsaffar, Department of Biology, College of Science, University of Baghdad, Al-Jaderyia Campus, Baghdad, Iraq, E-mail:
Correction note: Correction added after online publication July 2, 2021. Mistakenly one author’s name was given as Haider A. Rasheed instead of Haider Abdulhameed Alqaraghuli.

Acknowledgments

The authors acknowledge Dr. Hula Y. Alsadi for her assistance in molecular and bioinformatics part.

  1. Research funding: No external funds were used for this study.

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Written informed consent was obtained from all patients and control individuals.

  5. Ethical approval: Study was approved by the Ethical Committees of the Department of Biology, College of Science, University of Baghdad (Reference No. 1856).

References

1. Sethi, GS, Dharwal, V, Naura, AS. Immunological basis of oxidative stress-induced lung inflammation in asthma and COPD. Oxidative stress in lung diseases. Singapore: Springer Nature; 2019:195–223 pp.10.1007/978-981-13-8413-4_11Search in Google Scholar

2. Bonato, M, Bazzan, E, Snijders, D, Tinè, M, Biondini, D, Turato, G, et al.. Clinical and pathologic factors predicting future asthma in wheezing children. A longitudinal study. Am J Respir Cell Mol Biol 2018;59:458–66. https://doi.org/10.1165/rcmb.2018-0009oc.Search in Google Scholar

3. Gibeon, D, Menzies-Gow, AN. Targeting interleukins to treat severe asthma. Expet Rev Respir Med 2012;6:423–39. https://doi.org/10.1586/ers.12.38.Search in Google Scholar PubMed

4. Ali Naeem, S, Hind Hussein, S. Investigate the relation between polymorphism of IL-13 gene and asthma at Thi-Qar province/Iraq. J Educ Pure Sci 2015;5:212–30.Search in Google Scholar

5. Ayad, MG, Ahmed, AA, Mohammed, AH, Hashim, MH. Interleukin-4 single nucleotide polymorphism C-590T polymorphisms in relation to asthma. Iraqi J Med Sci 2018;16:51–6.10.22578/IJMS.16.1.8Search in Google Scholar

6. Alangari, AA. Corticosteroids in the treatment of acute asthma. Ann Thorac Med 2014;9:187. https://doi.org/10.4103/1817-1737.140120.Search in Google Scholar PubMed PubMed Central

7. Rao, S, Yao, Y, Ryan, J, Li, T, Wang, D, Zheng, C, et al.. Common variants in FKBP5 gene and major depressive disorder (MDD) susceptibility: a comprehensive meta-analysis. Sci Rep 2016;6:32687. https://doi.org/10.1038/srep32687.Search in Google Scholar PubMed PubMed Central

8. Menke, A, Klengel, T, Rubel, J, Brückl, T, Pfister, H, Lucae, S, et al.. Genetic variation in FKBP5 associated with the extent of stress hormone dysregulation in major depression. Gene Brain Behav 2013;12:289–96. https://doi.org/10.1111/gbb.12026.Search in Google Scholar PubMed

9. Molnár, Á, Patócs, A, Likó, I, Nyírő, G, Rácz, K, Tóth, M, et al.. An unexpected, mild phenotype of glucocorticoid resistance associated with glucocorticoid receptor gene mutation case report and review of the literature. BMC Med Genet 2018;19:1–6. https://doi.org/10.1186/s12881-018-0552-6.Search in Google Scholar PubMed PubMed Central

10. Zannas, A, Binder, E. Gene–environment interactions at the FKBP5 locus: sensitive periods, mechanisms and pleiotropism. Gene Brain Behav 2014;13:25–37. https://doi.org/10.1111/gbb.12104.Search in Google Scholar PubMed

11. Lavebratt, C, Åberg, E, Sjöholm, LK, Forsell, Y. Variations in FKBP5 and BDNF genes are suggestively associated with depression in a Swedish population-based cohort. J Affect Disord 2010;125:249–55. https://doi.org/10.1016/j.jad.2010.02.113.Search in Google Scholar PubMed

12. Tamman, AJ, Sippel, LM, Han, S, Neria, Y, Krystal, JH, Southwick, SM, et al.. Attachment style moderates effects of FKBP5 polymorphisms and childhood abuse on post-traumatic stress symptoms: results from the National Health and Resilience in Veterans Study. World J Biol Psychiatr 2019;20:289–300. https://doi.org/10.1080/15622975.2017.1376114.Search in Google Scholar PubMed

13. Hernandez-Diaz, Y, González-Castro, TB, Tovilla-Zárate, CA, Juárez-Rojop, IE, López-Narváez, ML, Pérez-Hernández, N, et al.. Association between FKBP5 polymorphisms and depressive disorders or suicidal behavior: a systematic review and meta-analysis study. Psychiatr Res 2019;271:658–68. https://doi.org/10.1016/j.psychres.2018.12.066.Search in Google Scholar PubMed

14. Alsaffar, SF, Yenzeel, JH, Ghazi, HF. Immunocytochemical assessment of FKBP51 and glucocorticoid receptor localization in asthmatic patients. Iraqi J Sci 2019;60:2609–17. https://doi.org/10.24996/ijs.2019.60.12.9.Search in Google Scholar

15. Alsaffar, SF, Yenzeel, JH, GHazi, HF. Immunocytochemical evaluation of FKBP51 protein and its relation to some hormonal, immunological and molecular aspects in glucocorticoid treated asthmatic patients. Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq; 2019.Search in Google Scholar

16. Sanger, F, Nicklen, S, Coulson, AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci 1977;74:5463–7. https://doi.org/10.1073/pnas.74.12.5463.Search in Google Scholar PubMed PubMed Central

17. Thomson, NC. Addressing corticosteroid insensitivity in adults with asthma. Expet Rev Respir Med 2016;10:137–56. https://doi.org/10.1586/17476348.2016.1133304.Search in Google Scholar PubMed

18. Leistner, C, Menke, A. How to measure glucocorticoid receptor’s sensitivity in patients with stress-related psychiatric disorders. Psychoneuroendocrinology 2018;91:235–60. https://doi.org/10.1016/j.psyneuen.2018.01.023.Search in Google Scholar PubMed

19. Alsaffar, SF, Ghazi, HF, Yenzeel, JH. FKBP51 immunocytochemical evaluation in induced sputum cells of Iraqi asthmatic patients. Biochem Cell Arch 2020;20:5147–54.Search in Google Scholar

20. Maltese, P, Palma, L, Sfara, C, De Rocco, P, Latiano, A, Palmieri, O, et al.. Glucocorticoid resistance in Crohn’s disease and ulcerative colitis: an association study investigating GR and FKBP5 gene polymorphisms. Pharmacogenomics J 2012;12:432–8. https://doi.org/10.1038/tpj.2011.26.Search in Google Scholar PubMed

21. Lao, W, Fang, M, Yang, X. FK506-binding protein 51 (FKBP5) gene polymorphism is not associated with glucocorticoid therapy outcome in patients with idiopathic thrombocytopenic purpura. Mol Med Rep 2012;6:787–90. https://doi.org/10.3892/mmr.2012.993.Search in Google Scholar PubMed

22. Sharma, S, Kho, AT, Chhabra, D, Qiu, W, Gaedigk, R, Vyhlidal, CA, et al.. Glucocorticoid genes and the developmental origins of asthma susceptibility and treatment response. Am J Respir Cell Mol Biol 2015;52:543–53. https://doi.org/10.1165/rcmb.2014-0109oc.Search in Google Scholar PubMed PubMed Central

23. Hawkins, GA, Lazarus, R, Smith, RS, Tantisira, KG, Meyers, DA, Peters, SP, et al.. The glucocorticoid receptor heterocomplex gene STIP1 is associated with improved lung function in asthmatic subjects treated with inhaled corticosteroids. J Allergy Clin Immunol 2009;123:1376–83.e7. https://doi.org/10.1016/j.jaci.2009.01.049.Search in Google Scholar PubMed PubMed Central

24. Russo, P, Tomino, C, Santoro, A, Prinzi, G, Proietti, S, Kisialiou, A, et al.. FKBP5 rs4713916: a potential genetic predictor of interindividual different response to inhaled corticosteroids in patients with chronic obstructive pulmonary disease in a real-life setting. Int J Mol Sci 2019;20:2024. https://doi.org/10.3390/ijms20082024.Search in Google Scholar PubMed PubMed Central

Received: 2021-01-13
Accepted: 2021-03-31
Published Online: 2021-06-25

© 2021 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/jbcpp-2020-0450
Keywords for this article
asthma; corticosteroid resistance; FKBP5 polymorphism

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  1. Frontmatter
  2. Original Articles
  3. Cost of illness of diabetes mellitus in Indonesia: a systematic review
  4. Social media health interventions to improve diabetes mellitus patient outcome: a systematic review
  5. Developing pharmacokinetics–pharmacodynamics model of valproic acid syrup based on prediction of population pharmacokinetics parameter and seizure frequency in Indonesian pediatric epilepsy outpatients
  6. Acetylcholinesterase inhibitory activity of extract and fractions from the root of Rauvolfia serpentina(L.) Bth.ex Kurz
  7. Green tea and its active compound epigallocathechin-3-gallate (EGCG) inhibit neuronal apoptosis in a middle cerebral artery occlusion (MCAO) model
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  17. Thymoquinone and its derivatives against breast cancer with HER2 positive: in silico studies of ADMET, docking and QSPR
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  19. The development and validation of the health belief model questionnaire for measuring factors affecting adherence in the elderly with hypertension
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