Home AST/ALT levels, MDA, and liver histopathology of Echinometra mathaei ethanol extract on paracetamol-induced hepatotoxicity in rats
Article
Licensed
Unlicensed Requires Authentication

AST/ALT levels, MDA, and liver histopathology of Echinometra mathaei ethanol extract on paracetamol-induced hepatotoxicity in rats

  • Angelica Kresnamurti ORCID logo EMAIL logo , Dita Nurlita Rakhma , Amitasari Damayanti , Septiyan Dwi Santoso , Enggar Restryarto , Wifqi Hadinata and Iwan Sahrial Hamid
Published/Copyright: June 25, 2021
Journal of Basic and Clinical Physiology and Pharmacology
From the journal Journal of Basic and Clinical Physiology and Pharmacology Volume 32 Issue 4

Abstract

Objectives

Echinometra mathaei was known to have potential antioxidant activities because it contains of polyhydroxy-naphthoquinone (echinochrome and spinochromes). The antioxidant properties contributed to the hepatoprotective effect by binding to free radicals compound that causes oxidative stress and necrosis in the hepatocytes. The research aimed to determine the hepatorepair effects of the E. mathaei ethanol extract on high-dose paracetamol-induced hepatic damage in Wistar rats.

Methods

This research used a true experimental method. Thirty white male rats were divided into sixth groups, i.e., normal control group, group II–VI was induced paracetamol 2,000 mg/kg BW for three days. After paracetamol-induced, group III–VI was treated with curcumin 800 mg/kg BW, E. mathaei extract 400, 800, and 1,200 mg/kg BW for seven days. The hepatorepair parameter was obtained from AST/ALT, MDA tissue levels, and the number of hepatocyte necrosis cells. The data results were analyzed using the ANOVA test, followed by the LSD test to determine the difference between each treatment.

Results

The results showed that E. mathaei significantly (p<0.05) decreased the AST levels, MDA levels and the number of hepatocyte necrosis cells at a dose of 800 mg/kg BW per orally treatment.

Conclusions

The E. mathaei ethanol extract repaired the hepatic damage induced by paracetamol.

Keywords: AST/ALT; Echinometra mathaei ; hepatocyte necrotic cell; hepatorepair; MDA; paracetamol-induced; sea urchin; Wistar rat
Corresponding author: Angelica Kresnamurti, Pharmacy Study Program, Faculty of Medicine, Hang Tuah University, Surabaya, Indonesia, Phone: +62-82123341711, E-mail:

Funding source: Hang Tuah University-Internal Research Grant

  1. Research funding: This research had fully funded from the internal research grant from Hang Tuah University. The funding organization played no role in study design: in the collection, analysis, and the interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Not applicable.

  5. Ethical approval: The research related to animals used has complied with all the relevant national regulations and institutional policies for the care and use of animals (Ethical Clearance Committee for Preclinical Research, Faculty of Dentistry, Hang Tuah University, Indonesia, and obtained approval number code: S.Ket/035/KEPK-FKGUHT/XI/2019).

References

1. Mahmoodzadeh, Y, Mazani, M, Rezagholizadeh, L. Hepatorepair effect of methanolic Tanacetum parthenium extract on CCl4-induced liver damage in rats. Toxicol Rep 2017;4:455–62. https://doi.org/10.1016/j.toxrep.2017.08.003.Search in Google Scholar PubMed PubMed Central

2. Wardani, G, Farida, N, Andayani, R, Kuntoro, M, Sudjarwo, SA. The potency of red seaweed (Eucheuma cottonii) extracts as hepatoprotector on lead acetate-induced hepatotoxicity in mice. Pharmacogn Res 2017;9:282–6. https://doi.org/10.4103/pr.pr_69_16.Search in Google Scholar PubMed PubMed Central

3. Adam, GO, Rahman, MM, Lee, SJ, Kim, GB, Kang, HS, Kim, JS, et al.. Hepatorepair effects of Nigella sativa seed extract against acetaminophen-induced oxidative stress. Asian Pac J Trop Med 2016;9:221–7. https://doi.org/10.1016/j.apjtm.2016.01.039.Search in Google Scholar PubMed

4. Całyniuk, B, Grochowska-Niedworok, E, Walkiewicz, KW, Kawecka, S, Popiołek, E, Fatyga, E. Malondialdehyde (MDA)–product of lipid peroxidation as marker of homeostasis disorders and aging. Ann Acad Med Siles 2016;70:224–8.10.18794/aams/65697Search in Google Scholar

5. Grattagliano, I, Bonfrate, L, Diogo, CV, Wang, HH, Wang, DQH, Portincasa, P. Biochemical mechanisms in drug-induced liver injury: certainties and doubts. World J Gastroenterol 2009;15:4865–76. https://doi.org/10.3748/wjg.15.4865.Search in Google Scholar PubMed PubMed Central

6. Cha, H, Lee, S, Lee, JH, Park, JW. Protective effects of p-coumaric acid against acetaminophen-induced hepatotoxicity in mice. Food Chem Toxicol 2018;121:131–9. https://doi.org/10.1016/j.fct.2018.08.060.Search in Google Scholar PubMed

7. Härmä, A, Aikio, O, Hallman, M, Saarela, T. Intravenous paracetamol decreases requirements of morphine in very preterm infants. J Pediatr 2016;168:36–40. https://doi.org/10.1016/j.jpeds.2015.08.003.Search in Google Scholar PubMed

8. Ghobadi, S, Dastan, D, Soleimani, M, Nili-Ahmadabadi, A. Hepatorepair potential and antioxidant activity of Allium tripedale in acetaminophen-induced oxidative damage. Res Pharmaceut Sci 2019;14:488.10.4103/1735-5362.272535Search in Google Scholar PubMed PubMed Central

9. Du, K, Ramachandran, A, Jaeschke, H. Oxidative stress during acetaminophen hepatotoxicity: sources, pathophysiological role and therapeutic potential. Redox Biol 2016;10:148–56. https://doi.org/10.1016/j.redox.2016.10.001.Search in Google Scholar PubMed PubMed Central

10. Xie, W, Wang, M, Chen, C, Zhang, X, Melzig, MF. Hepatorepair effect of isoquercitrin against acetaminophen-induced liver injury. Life Sci 2016;152:180–9. https://doi.org/10.1016/j.lfs.2016.04.002.Search in Google Scholar PubMed

11. Tzankova, V, Aluani, D, Kondeva-Burdina, M, Yordanov, Y, Odzhakov, F, Apostolov, A, et al.. Hepatorepair and antioxidant activity of quercetin loaded chitosan/alginate particles in vitro and in vivo in a model of paracetamol-induced toxicity. Biomed Pharmacother 2017;92:569–79. https://doi.org/10.1016/j.biopha.2017.05.008.Search in Google Scholar PubMed

12. Kuwahara, R, Hatate, H, Yuki, T, Murata, H, Tanaka, R, Hama, Y. Antioxidant property of polyhydroxylated naphthoquinone pigments from shells of purple sea urchin Anthocidaris crassispina. LWT – Food Sci Technol 2019;42:1296–300.10.1016/j.lwt.2009.02.020Search in Google Scholar

13. Popov, AM, Krivoshapko, ON. Protective effects of polar lipids and redox-active compounds from marine organisms at modeling of hyperlipidemia and diabetes. J Biomed Sci Eng 2013;6:543–50. https://doi.org/10.4236/jbise.2013.65069.Search in Google Scholar

14. Soleimani, S, Moein, S, Yousefzadi, M, Bioki, NA. Determination of in vitro antioxidant properties, anti-inflammatory effects and A-amylase inhibition of purple sea urchin extract of Echinometra mathaei from the Persian Gulf. Jundishapur J Nat Pharm Prod 2016;12:e36547. https://doi.org/10.5812/jjnpp.36547.Search in Google Scholar

15. Mohamed, AS, Sadek, SA, Hassanein, SS, Soliman, AM. Hepatorepair effect of echinochrome pigment in septic rats. J Surg Res 2019;234:317–24. https://doi.org/10.1016/j.jss.2018.10.004.Search in Google Scholar PubMed

16. Adhvaryu, MR, Reddy, NM, Vakharia, BC, Vaidya, B. Prevention of hepatotoxicity due to anti tuberculosis treatment: a novel integrative approach. World J Gastroenterol 2008;14:4753–62. https://doi.org/10.3748/wjg.14.4753.Search in Google Scholar PubMed PubMed Central

17. Hosein, FM, Zobeiri, M, Parvizi, F, El-Senduny, FF, Marmouzi, I, Coy-Barrera, E, et al.. Curcumin in liver diseases: a systematic review of the cellular mechanisms of oxidative stress and clinical perspective. Nutrients 2018;10:855.10.3390/nu10070855Search in Google Scholar PubMed PubMed Central

18. Alam, J, Mujahid, M, Jahan, Y, Bagga, P, Rahman, MA. Hepatorepair potential of ethanolic extract of Aquilaria agallocha leaves against paracetamol induced hepatotoxicity in SD rats. J Tradit Complementary Med 2017;7:9–13. https://doi.org/10.1016/j.jtcme.2015.12.006.Search in Google Scholar PubMed PubMed Central

19. Sharma, S, Chaturvedi, J, Chaudhari, BP, Singh, RL, Kakkar, P. Probiotic Enterococcus lactis IITRHR1 protects against acetaminophen-induced hepatotoxicity. Nutrition 2012;28:173–81. https://doi.org/10.1016/j.nut.2011.02.012.Search in Google Scholar PubMed

20. Singh, H, Prakash, A, Kalia, AN, Majeed, AB. Synergistic hepatoprotective potential of ethanolic extract of Solanum xanthocarpum and Juniperus communis against paracetamol and azithromycin induced liver injury in rats. J Tradit Complementary Med 2016;6:370–6. https://doi.org/10.1016/j.jtcme.2015.07.005.Search in Google Scholar PubMed PubMed Central

21. Mian, P, Knibbe, CA, Calvier, EA, Tibboel, D, Allegaert, K. Intravenous paracetamol dosing guidelines for pain management in (pre) term neonates using the paediatric study decision tree. Curr Pharmaceut Des 2017;23:5839–49.10.2174/1381612823666170921143104Search in Google Scholar PubMed

22. Tafere, GG, Tuem, KB, Gebre, AK, Balasubramaniam, R. In vitro antioxidant and in vivo hepatoprotective activities of root bark extract and solvent fractions of Croton macrostachyus Hochst. Ex Del. (Euphorbiaceae) on paracetamol-induced liver damage in mice. J Exp Pharmacol 2020;12:301. https://doi.org/10.2147/JEP.S259081.Search in Google Scholar PubMed PubMed Central

23. Senthilkumar, R, Chandran, R, Parimelazhagan, T. Hepatoprotective effect of Rhodiola imbricatarhizome against paracetamol-induced liver toxicity in rats. Saudi J Biol Sci 2014;21:409–16. https://doi.org/10.1016/j.sjbs.2014.04.001.Search in Google Scholar PubMed PubMed Central

24. Lin, CY, Ding, HJ, Lin, T, Lin, CC, Kuo, TH, Kao, CH. Positive correlation between serum liver enzyme levels and standard uptake values of liver on FDG-PET. Clin Imag 2010;34:109–12. https://doi.org/10.1016/j.clinimag.2009.05.007.Search in Google Scholar PubMed

25. Shanmugam, S, Thangaraj, P, Lima, BS, Chandran, R, Araújo, AAS, Narain, N, et al.. Effects of luteolin and quercetina-3-B-D-glucoside identified from Passiflora subpeltata leaves against acetaminophen induced hepatotoxicity in rats. Biomed Pharmacother 2016;83:1278–85. https://doi.org/10.1016/j.biopha.2016.08.044.Search in Google Scholar PubMed

26. Sharifudin, SA, Fakurazi, S, Hidayat, MT, Hairuszah, I, Aris Mohd Moklas, M, Arulselvan, P. Therapeutic potential of Moringa oleifera extracts against acetaminophen-induced hepatotoxicity in rats. Pharmaceut Biol 2013;51:279–88. https://doi.org/10.3109/13880209.2012.720993.Search in Google Scholar PubMed

27. Maronpot, RR, Yoshizawa, K, Nyska, A, Harada, T, Flake, G, Mueller, G, et al.. Hepatic enzyme induction: histopathology. Toxicol Pathol 2010;38:776–95. https://doi.org/10.1177/0192623310373778.Search in Google Scholar PubMed

28. Soleimani, S, Yousefzadi, M, Rezadoost, H, Bioki, NA. Identification and antioxidant of polyhydroxylated naphthoquinone pigments from sea urchin pigments of Echinometra mathaei. Med Chem Res 2016;25:1476–83. https://doi.org/10.1007/s00044-016-1586-y.Search in Google Scholar

29. Kavitha, P, Ramesh, R, Bupesh, G, Stalin, A, Subramanian, P. Hepatoprotective activity of Tribulus terrestris extract against acetaminophen-induced toxicity in a freshwater fish (Oreochromis mossambicus). Vitro Anim Cell Dev Biol 2011;47:698–706. https://doi.org/10.1007/s11626-011-9457-9.Search in Google Scholar PubMed

30. Sohair, RF, Dawlat, AS, Amel, MS, Nesreen, YA, Wafaa, EA. Protective effect of echinochrome against intrahepatic cholestasis induced by alpha-naphtylisothiocyanate in rats. Braz J Biol 2020;80:102–11.10.1590/1519-6984.192697Search in Google Scholar PubMed

31. Sayed, DA, Soliman, AM, Fahmy, SR. Echinochrome pigment as novel therapeutic agent against experimentally-induced gastric ulcer in rats. Biomed Pharmacother 2018;107:90–5. https://doi.org/10.1016/j.biopha.2018.07.173.Search in Google Scholar PubMed

Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/jbcpp-2020-0420).

Received: 2020-11-27
Accepted: 2021-03-08
Published Online: 2021-06-25

© 2021 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Frontmatter
  2. Original Articles
  3. Cost of illness of diabetes mellitus in Indonesia: a systematic review
  4. Social media health interventions to improve diabetes mellitus patient outcome: a systematic review
  5. Developing pharmacokinetics–pharmacodynamics model of valproic acid syrup based on prediction of population pharmacokinetics parameter and seizure frequency in Indonesian pediatric epilepsy outpatients
  6. Acetylcholinesterase inhibitory activity of extract and fractions from the root of Rauvolfia serpentina(L.) Bth.ex Kurz
  7. Green tea and its active compound epigallocathechin-3-gallate (EGCG) inhibit neuronal apoptosis in a middle cerebral artery occlusion (MCAO) model
  8. The effects of quercetin on nicotine-induced reward effects in mice
  9. Resveratrol ameliorates physical and psychological stress-induced depressive-like behavior
  10. Translation and cross-cultural adaption of an instrument measuring patient’s well-being under treatment for schizophrenia
  11. Quercetin promotes behavioral recovery and biomolecular changes of melanocortin-4 receptor in mice with ischemic stroke
  12. Knowledge and attitudes of healthcare professionals on prescribing errors
  13. Inhibition of Ras and STAT3 activity of 4-(tert-butyl)-N-carbamoylbenzamide as antiproliferative agent in HER2-expressing breast cancer cells
  14. Predicting the molecular mechanism of glucosamine in accelerating bone defect repair by stimulating osteogenic proteins
  15. Larvicidal toxicity and parasporal inclusion of native Bacillus thuringiensis BK5.2 against Aedes aegypti
  16. Synthesis, ADMET predictions, molecular docking studies, and in-vitro anticancer activity of some benzoxazines against A549 human lung cancer cells
  17. Thymoquinone and its derivatives against breast cancer with HER2 positive: in silico studies of ADMET, docking and QSPR
  18. Assessment of patient understanding of their conventional cardiac medicines and herbal prepared/derived products: preliminary survey and interviews with selected community-dwelling elderly patients in the Philippines
  19. The development and validation of the health belief model questionnaire for measuring factors affecting adherence in the elderly with hypertension
  20. Analysis of the side effect of QTc interval prolongation in the bedaquiline regimen in drug resistant tuberculosis patients
  21. Shallot skin profilling, computational evaluation of physicochemical properties, ADMET, and molecular docking of its components against P2Y12 receptor
  22. Analysis of HMGB-1 level before and after providing atorvastatin standard therapy in coronary artery disease patients with type-2 diabetes mellitus compared to without type-2 diabetes mellitus
  23. Analysis of matrix metalloproteinase-9 levels among acute heart failure patients with ACE inhibitor therapy (Dr. Soetomo Regional General Hospital, Surabaya)
  24. The correlation between self-related adherence, asthma-related quality of life and control of asthma in adult patients
  25. Providing counseling through home pharmacy care (HPC) for hemodialysis patients with hypertension in lowering blood pressure
  26. Community knowledge and attitude in recognizing asthma symptoms and using medication for asthma attacks: a cross-sectional study
  27. A study of anticoagulant therapy in patients with coronary artery disease
  28. The association of FKBP5 polymorphism with asthma susceptibility in asthmatic patients
  29. Gastroprotective effect of fluvoxamine and ondansetron on stress-induced gastric ulcers in mice
  30. Osteoblast iron genes: real time PCR and microarray hybridization approach under hyperoxia
  31. Attenuation of hyperplasia in lung parenchymal and colonic epithelial cells in DMBA-induced cancer by administering Andrographis paniculata Nees extract using animal model
  32. N-nitrosodiethylamine induces inflammation of liver in mice
  33. AST/ALT levels, MDA, and liver histopathology of Echinometra mathaei ethanol extract on paracetamol-induced hepatotoxicity in rats
  34. Development, characterization, molecular docking, and in vivo skin penetration of coenzyme Q10 nanostructured lipid carriers using tristearin and stearyl alcohol for dermal delivery
  35. The effect of Camellia sinensis (green tea) with its active compound EGCG on neuronal cell necroptosis in Rattus norvegicus middle cerebral artery occlusion (MCAO) model
  36. Hepatoprotective effect of ethanolic extract of sugarcane (Saccharum officinarum Linn.) leaves
  37. Correlation between the exposure time to mobile devices and the prevalence of evaporative dry eyes as one of the symptoms of computer vision syndrome among Senior High School students in East Java, Indonesia
  38. The effect of various high-fat diet on liver histology in the development of NAFLD models in mice
  39. Fabrication and characterization of bovine hydroxyapatite-gelatin-alendronate scaffold cross-linked by glutaraldehyde for bone regeneration
  40. Health related quality of life among postmenopausal woman with hormone responsive HER2− breast cancer in Indonesia
  41. Gender differences in the blood glucose type 2 diabetes patients with combination rapid and long acting insulin therapy
  42. Correlation of dietary iron intake and serum iron with thyroid stimulating hormone (TSH) and free thyroxine (FT4) levels in adult hyperthyroid patients
  43. The effect of pillbox use and education by pharmacist toward medication adherence in diabetes mellitus patients in a Primary Health Care Center in Mataram
  44. Variation concentration effect of propyleneglycol, glycerin, and polyethyleneglycol 400 to physical properties and dissolution rate of loratadine liquisolid tablet
  45. Role of Centella asiatica and ceramide in skin barrier improvement: a double blind clinical trial of Indonesian batik workers
  46. Secondary metabolite and antipyretic effects of Maja (Crescentia cujete L.) in fever-induced mice
  47. Hydration effect on kidney function and serum electrolyte in children with tumor lysis syndrome (TLS) and risk of TLS
  48. Drug utilization study and cost analysis of adult β-thalassemia major patient therapy at Dr. Soetomo General Hospital Surabaya
  49. The role of hyperbaric oxygen to platelet aggregation in noninsulin-dependent diabetes mellitus (NIDDM)
  50. Cocrystal formation of loratadine-succinic acid and its improved solubility
  51. The role of chondroitin sulfate to bone healing indicators and compressive strength
  52. The effects of quercetin on the expression of SREBP-1c mRNA in high-fat diet-induced NAFLD in mice
  53. Analysis of stress ulcer prophylaxis drug regimentation in surgical patients
  54. The stability and irritability study of the chitosan–Aloe vera spray gel as wound healing
  55. Effectiveness of citicoline in pediatric patients with refractive amblyopia in Surabaya, East Java, Indonesia
  56. The thermodynamic study of p-methoxycinnamic acid inclusion complex formation, using β-cyclodextrin and hydroxypropyl-β-cyclodextrin
  57. The effect of chitosan type and drug-chitosan ratio on physical characteristics and release profile of ketoprofen microparticles prepared by spray drying
  58. The maximum dose and duration in the therapy single use methotrexate to achieve remission by rheumatoid arthritis patients through disease activity score 28 (DAS28)
  59. Knowledge, attitudes, and practices (KAP) towards COVID-19 among university students in Pakistan: a cross-sectional study
  60. The impact of glutaraldehyde on the characteristics of bovine hydroxyapatite-gelatin based bone scaffold as gentamicin delivery system
  61. Analysis of the use of antibiotics profile and factors of surgical site infections study on digestive and oncology surgeries
  62. Second internal transcribed spacer (ITS-2) as genetic marker for molecular characterization of Sarcoptes scabiei in rabbits from several areas of East Java, Indonesia
  63. Design of gossypetin derivatives based on naturally occurring flavonoid in Hibiscus sabdariffa and the molecular docking as antibacterial agents
  64. Discovery of new targeting agents against GAPDH receptor for antituberculosis drug delivery
  65. The effect of red passion fruit (Passiflora edulis Sims.) fermentation time on its activity against Extended Strain Methicillin-Resistant (ESBL) Escherichia coli and Methicillin-Resistant Staphylococcus aureus (MRSA)
  66. Antibiotic use on acute respiratory tract infection nonpneumonia and nonspecific diarrhea in Primary Health Care Centre in Banjarbaru City, South Kalimantan, Indonesia
  67. Screening of anti-HIV activities in ethanol extract and fractions from Ficus fistulosa leaves
  68. The characteristics of lactic acid bacteria isolated from fermented food as potential probiotics
  69. Profile of gyrA gene mutation in clinical isolate of levofloxacin resistant Escherichia coli
  70. Antimicrobial activity of Centella asiatica and Gigantochloa apus
  71. Drug-related problems of antibiotic use in gastroenteritis related to patient therapy outcomes at Universitas Gadjah Mada Hospital
  72. The impact of suitability of empirical antibiotics use on therapeutic outcome of respiratory tract infection patients at inpatient wards of Universitas Gadjah Mada Academic Hospital
  73. Genetic profile mutation rpoB in clinical isolate of rifampicin-resistant Staphylococcus aureus
  74. Hematological side effect analysis of linezolid in MDR-TB patients with individual therapy
  75. Adverse drug reaction and its management in tuberculosis patients with multidrug resistance: a retrospective study
  76. Analysis of prophylactic antibiotic use and risk factor of postoperative infection in urological surgery patients
  77. Molecular docking studies of Nigella sativa L and Curcuma xanthorrhiza Roxb secondary metabolites against histamine N-methyltransferase with their ADMET prediction
  78. Prediction of compounds with antiosteoporosis activity in Chrysophyllum cainito L. leaves through in silico approach
  79. Phyllanthin and hypophyllanthin, the isolated compounds of Phyllanthus niruri inhibit protein receptor of corona virus (COVID-19) through in silico approach
  80. Cratoxylum sumatranum stem bark exhibited antimalarial activity by Lactate Dehydrogenase (LDH) assay
  81. Endophytic fungi inhabiting Physalis angulata L. plant: diversity, antioxidant, and antibacterial activities of their ethyl acetate extracts
  82. Exploration of several plants from Baung Forest on bone formation cell models
  83. In vitro antimalarial activity of Garcinia parvifolia Miq. Stem extracts and fractions on Plasmodium falciparum lactate dehydrogenase (LDH) assay
  84. Antioxidant and antiviral potency of Begonia medicinalis fractions
  85. Artocarpus sericicarpus stem bark contains antimalarial substances against Plasmodium falciparum
  86. Formulation and characterization of Eleutherine palmifolia extract-loaded self-nanoemulsifying drug delivery system (SNEDDS)
  87. Analytical method for the determination of curcumin entrapped in polymeric micellar powder using HPLC
  88. Challenges in the provision of natural medicines by community pharmacists in East Java Province, Indonesia
  89. In vitro and in silico analysis of phytochemical compounds of 96% ethanol extract of semanggi (Marsilea crenata Presl.) leaves as a bone formation agent
  90. Inhibitory activity of Urena lobata leaf extract on alpha-amylase and alpha-glucosidase: in vitro and in silico approach
  91. Case Report
  92. Effect of hydrocortisone on hypocorticolism caused by pituitary adenoma
https://doi.org/10.1515/jbcpp-2020-0420
Keywords for this article
AST/ALT; Echinometra mathaei; hepatocyte necrotic cell; hepatorepair; MDA; paracetamol-induced; sea urchin; Wistar rat

Articles in the same Issue

  1. Frontmatter
  2. Original Articles
  3. Cost of illness of diabetes mellitus in Indonesia: a systematic review
  4. Social media health interventions to improve diabetes mellitus patient outcome: a systematic review
  5. Developing pharmacokinetics–pharmacodynamics model of valproic acid syrup based on prediction of population pharmacokinetics parameter and seizure frequency in Indonesian pediatric epilepsy outpatients
  6. Acetylcholinesterase inhibitory activity of extract and fractions from the root of Rauvolfia serpentina(L.) Bth.ex Kurz
  7. Green tea and its active compound epigallocathechin-3-gallate (EGCG) inhibit neuronal apoptosis in a middle cerebral artery occlusion (MCAO) model
  8. The effects of quercetin on nicotine-induced reward effects in mice
  9. Resveratrol ameliorates physical and psychological stress-induced depressive-like behavior
  10. Translation and cross-cultural adaption of an instrument measuring patient’s well-being under treatment for schizophrenia
  11. Quercetin promotes behavioral recovery and biomolecular changes of melanocortin-4 receptor in mice with ischemic stroke
  12. Knowledge and attitudes of healthcare professionals on prescribing errors
  13. Inhibition of Ras and STAT3 activity of 4-(tert-butyl)-N-carbamoylbenzamide as antiproliferative agent in HER2-expressing breast cancer cells
  14. Predicting the molecular mechanism of glucosamine in accelerating bone defect repair by stimulating osteogenic proteins
  15. Larvicidal toxicity and parasporal inclusion of native Bacillus thuringiensis BK5.2 against Aedes aegypti
  16. Synthesis, ADMET predictions, molecular docking studies, and in-vitro anticancer activity of some benzoxazines against A549 human lung cancer cells
  17. Thymoquinone and its derivatives against breast cancer with HER2 positive: in silico studies of ADMET, docking and QSPR
  18. Assessment of patient understanding of their conventional cardiac medicines and herbal prepared/derived products: preliminary survey and interviews with selected community-dwelling elderly patients in the Philippines
  19. The development and validation of the health belief model questionnaire for measuring factors affecting adherence in the elderly with hypertension
  20. Analysis of the side effect of QTc interval prolongation in the bedaquiline regimen in drug resistant tuberculosis patients
  21. Shallot skin profilling, computational evaluation of physicochemical properties, ADMET, and molecular docking of its components against P2Y12 receptor
  22. Analysis of HMGB-1 level before and after providing atorvastatin standard therapy in coronary artery disease patients with type-2 diabetes mellitus compared to without type-2 diabetes mellitus
  23. Analysis of matrix metalloproteinase-9 levels among acute heart failure patients with ACE inhibitor therapy (Dr. Soetomo Regional General Hospital, Surabaya)
  24. The correlation between self-related adherence, asthma-related quality of life and control of asthma in adult patients
  25. Providing counseling through home pharmacy care (HPC) for hemodialysis patients with hypertension in lowering blood pressure
  26. Community knowledge and attitude in recognizing asthma symptoms and using medication for asthma attacks: a cross-sectional study
  27. A study of anticoagulant therapy in patients with coronary artery disease
  28. The association of FKBP5 polymorphism with asthma susceptibility in asthmatic patients
  29. Gastroprotective effect of fluvoxamine and ondansetron on stress-induced gastric ulcers in mice
  30. Osteoblast iron genes: real time PCR and microarray hybridization approach under hyperoxia
  31. Attenuation of hyperplasia in lung parenchymal and colonic epithelial cells in DMBA-induced cancer by administering Andrographis paniculata Nees extract using animal model
  32. N-nitrosodiethylamine induces inflammation of liver in mice
  33. AST/ALT levels, MDA, and liver histopathology of Echinometra mathaei ethanol extract on paracetamol-induced hepatotoxicity in rats
  34. Development, characterization, molecular docking, and in vivo skin penetration of coenzyme Q10 nanostructured lipid carriers using tristearin and stearyl alcohol for dermal delivery
  35. The effect of Camellia sinensis (green tea) with its active compound EGCG on neuronal cell necroptosis in Rattus norvegicus middle cerebral artery occlusion (MCAO) model
  36. Hepatoprotective effect of ethanolic extract of sugarcane (Saccharum officinarum Linn.) leaves
  37. Correlation between the exposure time to mobile devices and the prevalence of evaporative dry eyes as one of the symptoms of computer vision syndrome among Senior High School students in East Java, Indonesia
  38. The effect of various high-fat diet on liver histology in the development of NAFLD models in mice
  39. Fabrication and characterization of bovine hydroxyapatite-gelatin-alendronate scaffold cross-linked by glutaraldehyde for bone regeneration
  40. Health related quality of life among postmenopausal woman with hormone responsive HER2− breast cancer in Indonesia
  41. Gender differences in the blood glucose type 2 diabetes patients with combination rapid and long acting insulin therapy
  42. Correlation of dietary iron intake and serum iron with thyroid stimulating hormone (TSH) and free thyroxine (FT4) levels in adult hyperthyroid patients
  43. The effect of pillbox use and education by pharmacist toward medication adherence in diabetes mellitus patients in a Primary Health Care Center in Mataram
  44. Variation concentration effect of propyleneglycol, glycerin, and polyethyleneglycol 400 to physical properties and dissolution rate of loratadine liquisolid tablet
  45. Role of Centella asiatica and ceramide in skin barrier improvement: a double blind clinical trial of Indonesian batik workers
  46. Secondary metabolite and antipyretic effects of Maja (Crescentia cujete L.) in fever-induced mice
  47. Hydration effect on kidney function and serum electrolyte in children with tumor lysis syndrome (TLS) and risk of TLS
  48. Drug utilization study and cost analysis of adult β-thalassemia major patient therapy at Dr. Soetomo General Hospital Surabaya
  49. The role of hyperbaric oxygen to platelet aggregation in noninsulin-dependent diabetes mellitus (NIDDM)
  50. Cocrystal formation of loratadine-succinic acid and its improved solubility
  51. The role of chondroitin sulfate to bone healing indicators and compressive strength
  52. The effects of quercetin on the expression of SREBP-1c mRNA in high-fat diet-induced NAFLD in mice
  53. Analysis of stress ulcer prophylaxis drug regimentation in surgical patients
  54. The stability and irritability study of the chitosan–Aloe vera spray gel as wound healing
  55. Effectiveness of citicoline in pediatric patients with refractive amblyopia in Surabaya, East Java, Indonesia
  56. The thermodynamic study of p-methoxycinnamic acid inclusion complex formation, using β-cyclodextrin and hydroxypropyl-β-cyclodextrin
  57. The effect of chitosan type and drug-chitosan ratio on physical characteristics and release profile of ketoprofen microparticles prepared by spray drying
  58. The maximum dose and duration in the therapy single use methotrexate to achieve remission by rheumatoid arthritis patients through disease activity score 28 (DAS28)
  59. Knowledge, attitudes, and practices (KAP) towards COVID-19 among university students in Pakistan: a cross-sectional study
  60. The impact of glutaraldehyde on the characteristics of bovine hydroxyapatite-gelatin based bone scaffold as gentamicin delivery system
  61. Analysis of the use of antibiotics profile and factors of surgical site infections study on digestive and oncology surgeries
  62. Second internal transcribed spacer (ITS-2) as genetic marker for molecular characterization of Sarcoptes scabiei in rabbits from several areas of East Java, Indonesia
  63. Design of gossypetin derivatives based on naturally occurring flavonoid in Hibiscus sabdariffa and the molecular docking as antibacterial agents
  64. Discovery of new targeting agents against GAPDH receptor for antituberculosis drug delivery
  65. The effect of red passion fruit (Passiflora edulis Sims.) fermentation time on its activity against Extended Strain Methicillin-Resistant (ESBL) Escherichia coli and Methicillin-Resistant Staphylococcus aureus (MRSA)
  66. Antibiotic use on acute respiratory tract infection nonpneumonia and nonspecific diarrhea in Primary Health Care Centre in Banjarbaru City, South Kalimantan, Indonesia
  67. Screening of anti-HIV activities in ethanol extract and fractions from Ficus fistulosa leaves
  68. The characteristics of lactic acid bacteria isolated from fermented food as potential probiotics
  69. Profile of gyrA gene mutation in clinical isolate of levofloxacin resistant Escherichia coli
  70. Antimicrobial activity of Centella asiatica and Gigantochloa apus
  71. Drug-related problems of antibiotic use in gastroenteritis related to patient therapy outcomes at Universitas Gadjah Mada Hospital
  72. The impact of suitability of empirical antibiotics use on therapeutic outcome of respiratory tract infection patients at inpatient wards of Universitas Gadjah Mada Academic Hospital
  73. Genetic profile mutation rpoB in clinical isolate of rifampicin-resistant Staphylococcus aureus
  74. Hematological side effect analysis of linezolid in MDR-TB patients with individual therapy
  75. Adverse drug reaction and its management in tuberculosis patients with multidrug resistance: a retrospective study
  76. Analysis of prophylactic antibiotic use and risk factor of postoperative infection in urological surgery patients
  77. Molecular docking studies of Nigella sativa L and Curcuma xanthorrhiza Roxb secondary metabolites against histamine N-methyltransferase with their ADMET prediction
  78. Prediction of compounds with antiosteoporosis activity in Chrysophyllum cainito L. leaves through in silico approach
  79. Phyllanthin and hypophyllanthin, the isolated compounds of Phyllanthus niruri inhibit protein receptor of corona virus (COVID-19) through in silico approach
  80. Cratoxylum sumatranum stem bark exhibited antimalarial activity by Lactate Dehydrogenase (LDH) assay
  81. Endophytic fungi inhabiting Physalis angulata L. plant: diversity, antioxidant, and antibacterial activities of their ethyl acetate extracts
  82. Exploration of several plants from Baung Forest on bone formation cell models
  83. In vitro antimalarial activity of Garcinia parvifolia Miq. Stem extracts and fractions on Plasmodium falciparum lactate dehydrogenase (LDH) assay
  84. Antioxidant and antiviral potency of Begonia medicinalis fractions
  85. Artocarpus sericicarpus stem bark contains antimalarial substances against Plasmodium falciparum
  86. Formulation and characterization of Eleutherine palmifolia extract-loaded self-nanoemulsifying drug delivery system (SNEDDS)
  87. Analytical method for the determination of curcumin entrapped in polymeric micellar powder using HPLC
  88. Challenges in the provision of natural medicines by community pharmacists in East Java Province, Indonesia
  89. In vitro and in silico analysis of phytochemical compounds of 96% ethanol extract of semanggi (Marsilea crenata Presl.) leaves as a bone formation agent
  90. Inhibitory activity of Urena lobata leaf extract on alpha-amylase and alpha-glucosidase: in vitro and in silico approach
  91. Case Report
  92. Effect of hydrocortisone on hypocorticolism caused by pituitary adenoma
Sign up now to receive a 20% welcome discount
Institutional Access
How does access work?
Have an idea on how to improve our website?
Please write us.
© 2025 De Gruyter Brill
Downloaded on 19.10.2025 from https://www.degruyterbrill.com/document/doi/10.1515/jbcpp-2020-0420/html
Scroll to top button