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Prediction of compounds with antiosteoporosis activity in Chrysophyllum cainito L. leaves through in silico approach

  • Burhan Ma’arif , Hilwa Fitri , Nisfatul Lailatus Saidah , Luqman Alfani Najib , Achmad Hamdan Yuwafi , Ria Ramadhani Dwi Atmaja , Fidia Rizkiah Inayatillah , Meilina Ratna Dianti EMAIL logo , Hening Laswati and Mangestuti Agil
Published/Copyright: June 25, 2021
Journal of Basic and Clinical Physiology and Pharmacology
From the journal Journal of Basic and Clinical Physiology and Pharmacology Volume 32 Issue 4

Abstract

Objectives

Estrogen deficiency causes various health problems in postmenopausal women, including osteoporosis. Phytoestrogen emerged as a potential alternative of estrogen with minimum side effects. The aims of this study were to analyze the metabolite profiling results of various extract of Chyrsophyllum cainito L. leaves, which contain phytoestrogen, through in silico study against 3OLS protein, an X-ray protein of ERβ, so it can predict the types of the phytoestrogen contents which have antiosteoporosis property.

Methods

In silico analysis was carried out for the compounds from the metabolite profiling data of C. cainito leaves from our previous study. The structure compounds from metabolite profiling results of various extract of C. cainito leaves were prepared with Avogadro 1.0.1 software, molecular docking was done using PyRx 0.8 software, and Biovia Discovery Studio Visualizer 2016 software was used to visualize the structure of compounds against 3OLS protein. The physicochemical characteristics of the compounds were analyzed using the SwissADME web tool.

Results

From in silico studies, it was known that there were total 11 compounds in C. cainito leaves that predicted as phytoestrogens which have ERβ agonist properties against 3OLS protein. The ERβ agonist was a compound that has parameters similar to 17β-estradiol in its interaction with 3OLS protein, which has a pharmacophore distance of 10.862 Å, and binding to amino acids His 475 and Glu 305 or Arg 346 at receptor-ligand docking simulation.

Conclusions

C. cainito leaves contain 11 compounds that are predicted to be phytoestrogens with ERβ agonist properties, which is responsible for antiosteoporosis activity.

Keywords: antiosteoporosis; Chrysophyllum cainito L.; in silico ; phytoestrogen
Corresponding author: Meilina Ratna Dianti, M.Kep., Ns., Department of Pharmacy, Maulana Malik Ibrahim State Islamic University, Malang, Indonesia, Phone: +6282221006667, E-mail:

Acknowledgments

Not applicable

  1. Research funding: None declared.

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission. Burhan Ma’arif: research background and methods. Hilwa Fitri: collecting references. Nisfatul Lailatus Saidah: Collecting research material. Luqman Alfani Najib: Design of in silico experiment. Achmad Hamdan Yuwafi: writing of the article. Ria Ramadhani Dwi Atmaja: data analysis. Fidia Rizkiah Inayatillah: data analysis. Meilina Ratna Dianti: data interpretation. Hening Laswati: Proofread content. Mangestuti Agil: Proofread content.

  3. Competing interests: Author state no conflict of interest.

  4. Informed consent: Author state no informed consent because there was no individuals included in this study.

  5. Ethical approval: Not applicable.

References

1. Ji, MX, Yu, Q. Primary osteoporosis in postmenopausal women. Chronic Dis Transl Med 2015;1. https://doi.org/10.1016/j.cdtm.2015.02.006.Search in Google Scholar

2. Villa, A, Vegeto, E, Poletti, A, Maggi, A. Esterogens, neuroinflammation and neurogeneration. Endocr Soc 2016.10.1210/er.2016-1007Search in Google Scholar PubMed PubMed Central

3. Vijayakumar, B. Osteoporosis: an under-recognized public health problem. J Local Glob Health Sci 2016.10.5339/jlghs.2016.2Search in Google Scholar

4. Gheita, TA, Hammam, N. Epidemiology and awareness of osteoporosis: a viewpoint from the Middle East and North Africa. Int J Clin Rheumatol 2013;13:134–14.Search in Google Scholar

5. Al-Hamam, NM, Al-Moaibed, GF, Alfayez, EH, Al-Mubaddil, MS, Alramadhan, NA. Prevalence and risk factors for osteoporotic fracture among adults with comorbidities in Al-Ahsaa, Saudi Arabia.J Fam Med Prim Care 2020;9:877–82.10.4103/jfmpc.jfmpc_982_19Search in Google Scholar PubMed PubMed Central

6. Cooper, C, Cole, ZA, Holroyd, CR, Earl, SC, Harvey, NC, Dennison, EM, et al.. Secular trends in the incidence of hip and other osteoporotic fractures. Osteoporos Int 2011; 22:1277–88. https://doi.org/10.1007/s00198-011-1601-6.Search in Google Scholar

7. Lee, WL, Tsui, KH, Seow, KM, Cheng, MH, Su, WH, Chen, CP, et al.. Hormone therapy for postmenopausal women and unanswered issue. Gynecol Minimal Invasive Ther 2013;2:13–17. https://doi.org/10.1016/j.gmit.2012.12.003.Search in Google Scholar

8. Khalid, AB, Krum, SA. Estrogen receptors alpha and beta in bone. Bone 2016;87:130–5. https://doi.org/10.1016/j.bone.2016.03.016.Search in Google Scholar

9. Cui, J, Shen, Y, Li, R. Estrogen synthesis and signaling pathways during aging: from periphery to brain. Trends Mol Med 2013;19:197–209. https://doi.org/10.1016/j.molmed.2012.12.007.Search in Google Scholar

10. Yang, TS, Wang, SY, Yang, YC, Su, CH, Lee, FK, Chen, SC, et al.. Effects of standardized phytoestrogen on Taiwanese menopausal women. Taiwan J Obstet Gynecol 2012;51:229–35. https://doi.org/10.1016/j.tjog.2012.04.011.Search in Google Scholar

11. Das, A, Dato, IR, Badaruddin, BN, Amiya, B. A brief review on Chrysophyllum cainito. J Pharm Herb Formula 2010;1:1–7.Search in Google Scholar

12. Ma’arif, B, Aditama, A, Muti’ah, R, Bhagawan, WS, Amiruddin, R, Rukiana. Profil metabolit berbagai ekstrak daun Chrysophyllum cainito L. menggunakan UPLC-QTOF-MS/MS. Jurnal Tumbuhan Obat Indonesia 2019;12:10–24.10.22435/jtoi.v12i1.672Search in Google Scholar

13. Ma’arif, B, Aditama, AP. Activity of 96% ethanol extract of Chrisophyllum cainito L. in increasing vertebrae trabecular osteoblast cell number in male mice. Asian J Pharm Clin Res 2019;12:286–8.10.22159/ajpcr.2019.v12i1.28994Search in Google Scholar

14. Wadood, A, Ahmed, N, Shah, L, Ahmad, A., Hassan, H, Sham, S. In silico drug design: an approach whish revolutionarised the drug discovery process. OA Drug Design Deliv 2013;1:3. https://doi.org/10.13172/2054-4057-1-1-1119.Search in Google Scholar

15. Sliwoski, G, Kothiwale, S, Meiler, J, Lowe, EW. Computational methods in drug discovery. Pharmacol Rev 2014;66:334–95. https://doi.org/10.1124/pr.112.007336.Search in Google Scholar

16. Noviardi, H, Fachrurrazie. Potensi senyawa bullatalisin sebagai inhibitor protein lukotrien A4 hidrolase pada kanker kolon secara in silico. Fitofarmaka 2015;5. https://doi.org/10.33751/jf.v5i2.410.Search in Google Scholar

17. Pinto, VS, Araújo, JSC, Silva, RC, Costa, GV, Cruz, JN, Neto, MFDA, et al.. In silico study to identify new antituberculosis molecules from natural sources by hierarchical virtual screening and molecular dynamics simulations. Pharmaceuticals 2019;12:36. https://doi.org/10.3390/ph12010036.Search in Google Scholar

18. Babu, E, Kanai, Y, Chairoungdua, A, Kim, DK, Iribe, Y, Tangtrongsup, S, et al.. Identification of a novel system L amino acid transporter structurally distinct from heterodimeric amino acid transporters. J Biol Chem 2003;278:43838–45. https://doi.org/10.1074/jbc.m305221200.Search in Google Scholar

19. Sherwood, L. Human physiology from cells to systems. Australia: Thomson; 2004.Search in Google Scholar

20. Wend, K, Wend, P, Krum, SA. Tissue specific effects of loss of estrogen during menopause and aging. Front Endocrinol 2012;3:19. https://doi.org/10.3389/fendo.2012.00019.Search in Google Scholar

21. Cauley, JA. Estrogen and bone health in men and women. Steroids 2015;99:11–5. https://doi.org/10.1016/j.steroids.2014.12.010.Search in Google Scholar

22. Sihombing, I, Wangko, S, Kalangi, SJR. Peran estrogen dalam remodeling tulang. Jurnal Biomedik 2012;4:18–28.10.35790/jbm.4.3.2012.1210Search in Google Scholar

23. Waters, KM, Gebhart, JB, Rickard, DJ. Potential roles of estrogen receptor A and B in the regulation of human osteoblast functions and gene expression. The Menopause at the Millenium. In: Proceding of the 9th International Menopause Society World Congress on Menopause, Yokohama, Japan. The clinical evaluation of chemotherapeutic agents in cancer. In: Macleod, CM, editor. Evaluation of chemotherapeutic agents. New York: Columbia University Press, 1999:191–205 pp.Search in Google Scholar

24. de Villiers, TJ. Bone health and osteoporosis in postmenopausal women. Best Pract Res Clin Obstet Gynaecol 2009;23:73–85. https://doi.org/10.1016/j.bpobgyn.2008.10.009.Search in Google Scholar

25. Ososki, AL, Kennelly, EJ. Phytoestrogens: a review of the present state of research. Phytother Res 2003;17:845–69. https://doi.org/10.1002/ptr.1364.Search in Google Scholar

26. Muchtaridi, M, Dermawan, D, Yusuf, M. Molecular docking, 3D structure-based pharmacophore modeling, and ADME prediction of alpha mangostin and its derivatives against estrogen receptor alpha. J Young Pharm 2018;10:252–9. https://doi.org/10.5530/jyp.2018.10.58.Search in Google Scholar

27. Hanwell, MD, Curtis, DE, Lonie, DC, Vandermeersch, T, Zurek, E, Hutchison, GR. Avogadro: an advanced semantic chemical editor, visualization and analysis platform. J Cheminf 2012;4:17. https://doi.org/10.1186/1758-2946-4-17.Search in Google Scholar

28. Sliwoski, G, Kothiwale, S, Meiler, J, Lowe, EWJr. Computational methods in drug discovery. Pharmacol Rev 2014;66:334–95. https://doi.org/10.1124/pr.112.007336.Search in Google Scholar

29. Trot, O, Olson, AJ. AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading. J Comput Chem 2010;31:455–61.10.1002/jcc.21334Search in Google Scholar PubMed PubMed Central

30. Siswandono, SB. Kimia medisinal. Surabaya: Airlangga University Press; 1995.Search in Google Scholar

31. Harish, BM, Devaraju, KS, Gopi, A, Saraswathi, R, Anushree, Babu, RL, et al.. In silico binding affinity study of calcineurin inhibitors to calcineurin and its close associates. Indian J Biotechnol 2013;12:213–7.Search in Google Scholar

32. Ghatol, SP, Verma, S, Agarwal, K, Sharon, A. Pharmacophore distance mapping and docking study of some benzimidazole analogs as A2A receptor antagonists. Int J Pharm Sci Drug Res 2010:2:71–7.Search in Google Scholar

33. Vasavi, CS, Goyal, A, Divya, G, Munusami, P. In silico analysis of interactions in heme binding proteins. Int J Pharm Pharmaceut Sci 2015;7.Search in Google Scholar

34. Suhud, F. Uji aktivitas in silico senyawa baru 1-benzil-3-benzoilurea induk dan tersubstitusi sebagai agen antiproliferatif. Jurnal Farmasi Indonesia 2015;7.Search in Google Scholar

35. Nogara, PA, Saraiva, RA, Bueno, DC, Lissner, LJ, Corte, CLD, Braga, MM, et al.. Virtual screening of acetylcholinesterase inhibitors using the Lipinski’s rule f five and ZINC databank. Biomed Res Int 2014.10.1155/2015/870389Search in Google Scholar PubMed PubMed Central

36. Shityakov, S, Salvador, E, Förster, C. In silico, in vitro, and in vivo methods to analyse drug permeation across the blood-brain barrier: a critical review. OA Anaesth 2013;1:13. https://doi.org/10.13172/2052-7853-1-2-913.Search in Google Scholar

37. Daina, A, Zoete, V. A boiled-egg to predict gastrointestinal absorption and brain penetration of small molecules. ChemMedChem 2016;11:1117–21. https://doi.org/10.1002/cmdc.201600182.Search in Google Scholar

38. Chagas, CM, Moss, S, Alisaraiea, L. Drug metabolites and their effects on the development of adverse reactions: revisiting Lipinski’s rule of five. Int J Pharm 2018;549:133–49. https://doi.org/10.1016/j.ijpharm.2018.07.046.Search in Google Scholar
Received: 2020-11-24
Accepted: 2021-02-05
Published Online: 2021-06-25

© 2021 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/jbcpp-2020-0393
Keywords for this article
antiosteoporosis; Chrysophyllum cainito L.; in silico; phytoestrogen

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  1. Frontmatter
  2. Original Articles
  3. Cost of illness of diabetes mellitus in Indonesia: a systematic review
  4. Social media health interventions to improve diabetes mellitus patient outcome: a systematic review
  5. Developing pharmacokinetics–pharmacodynamics model of valproic acid syrup based on prediction of population pharmacokinetics parameter and seizure frequency in Indonesian pediatric epilepsy outpatients
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  71. Drug-related problems of antibiotic use in gastroenteritis related to patient therapy outcomes at Universitas Gadjah Mada Hospital
  72. The impact of suitability of empirical antibiotics use on therapeutic outcome of respiratory tract infection patients at inpatient wards of Universitas Gadjah Mada Academic Hospital
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  80. Cratoxylum sumatranum stem bark exhibited antimalarial activity by Lactate Dehydrogenase (LDH) assay
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  82. Exploration of several plants from Baung Forest on bone formation cell models
  83. In vitro antimalarial activity of Garcinia parvifolia Miq. Stem extracts and fractions on Plasmodium falciparum lactate dehydrogenase (LDH) assay
  84. Antioxidant and antiviral potency of Begonia medicinalis fractions
  85. Artocarpus sericicarpus stem bark contains antimalarial substances against Plasmodium falciparum
  86. Formulation and characterization of Eleutherine palmifolia extract-loaded self-nanoemulsifying drug delivery system (SNEDDS)
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  89. In vitro and in silico analysis of phytochemical compounds of 96% ethanol extract of semanggi (Marsilea crenata Presl.) leaves as a bone formation agent
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