Effects of Onosma dichroanthum Boiss. root extract on AGS human gastric cancer cell-line
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Mohammad Mostakhdem Hashemi
,
Nahid Poursharifi
Abstract
One of the cancer-related deaths is gastric cancer in this area. Onosma dichroanthum Boiss. roots have been used as an antiseptic and anti-inflammatory for wound healing treatment. The aim of this study was to assess the in vitro cytotoxic and anticancer effects of O. dichroanthum Boiss. roots from the Golestan province of Iran. After identification of the taxonomical effect of O. dichroanthum Boiss., different concentrations of the hydroalcoholic root extract were used. Three different time periods (24, 48, and 72 h) were used to treat AGS gastric cancer and L-929 normal fibroblasts cell lines. The evaluation of different concentrations of root extract was performed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The 48 h treatment affected cell survival, while the concentration of 64 μg/mL was determined as IC50 concentrations at 48 h incubation time. The 48 h incubation time with 64 μg/mL showed the best effectiveness on cancerous cell-line while being safe for normal cell-line. Our results show that O. dichroanthum Boiss. roots extract may have cytotoxic and safe effects on gastric cancer cell-line and normal cells in 48 h treatment periods, respectively. The results indicated the O. dichroanthum Boiss. may be as an effective anticancer agent (gastric cancer).
Funding source: Golestan University of Medical Sciences
Funding source: Metabolic Disorders Research Center
Acknowledgments
The authors would like to thank the Research Deputy of the Golestan University of Medical Sciences for financial support. The authors present their deep thanks to Mr. Mohammad Mostakhdem Hashemi for his valuable help.
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Research funding: This work has been financially supported by the Research Deputy of the Golestan University of Medical Science and Metabolic Disorders Research Center.
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Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: Authors state no conflict of interest.
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Informed consent: The ethnic committee of the Golestan University of Medical Sciences approved the study (With ethics number: IR.GOUMS. 1394.312).
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Ethical approval: The ethnic committee of the Golestan University of Medical Sciences approval the study (With ethics number: IR.GOUMS. 1394.312).
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© 2021 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Minireview
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- Hyponatremia in heart failure: not just 135 to 145
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- Effects of berberine on cholinesterases and monoamine oxidase activities, and antioxidant status in the brain of streptozotocin (STZ)-induced diabetic rats
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- Terminalia arjuna supplementation ameliorates high fat diet-induced oxidative stress in nephrotoxic rats
- Decreased expression of annexin A2 and loss of its association with vascular endothelial growth factor leads to the deficient trophoblastic invasion in preeclampsia
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- Dose-dependent and time-dependent metabolic, hemodynamic, and redox disturbances in dexamethasone-treated Wistar rats
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- Effect of luteolin on the gene level expression of apoptosis-associated speck-like protein containing a caspase recruitment domain of NLRP3 inflammasome and NF-κB in rats subjected to experimental pancreatitis – influence of HSP70
- Effects of Onosma dichroanthum Boiss. root extract on AGS human gastric cancer cell-line
- Feasibility, face, and content validity of quantitative computed tomography in interstitial lung disease related to connective tissue diseases
- In vitro anticoagulant activity of selected medicinal plants: potential interactions with warfarin and development of new anticoagulants
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- Short Communication
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Articles in the same Issue
- Frontmatter
- Minireview
- Can pulse wave velocity (PWV) alone express arterial stiffness? A neglected tool for vascular function assessment
- Review
- Hyponatremia in heart failure: not just 135 to 145
- Original Articles
- Effects of berberine on cholinesterases and monoamine oxidase activities, and antioxidant status in the brain of streptozotocin (STZ)-induced diabetic rats
- Paradoxical sleep deprivation induces oxidative stress in the submandibular glands of Wistar rats
- Terminalia arjuna supplementation ameliorates high fat diet-induced oxidative stress in nephrotoxic rats
- Decreased expression of annexin A2 and loss of its association with vascular endothelial growth factor leads to the deficient trophoblastic invasion in preeclampsia
- Algogen-induced vasosensory reflexes modulate short-term heart rate variability parameters in experimental rat models
- Assessment of sleep quality and its predictors among newly diagnosed psychiatric patients
- Ovalbumin/lipopolysaccharide induced vasculitis in rats: a new predictive model
- Dose-dependent and time-dependent metabolic, hemodynamic, and redox disturbances in dexamethasone-treated Wistar rats
- Effects of sleeve gastrectomy on neutrophil–lymphocyte ratio
- Effect of luteolin on the gene level expression of apoptosis-associated speck-like protein containing a caspase recruitment domain of NLRP3 inflammasome and NF-κB in rats subjected to experimental pancreatitis – influence of HSP70
- Effects of Onosma dichroanthum Boiss. root extract on AGS human gastric cancer cell-line
- Feasibility, face, and content validity of quantitative computed tomography in interstitial lung disease related to connective tissue diseases
- In vitro anticoagulant activity of selected medicinal plants: potential interactions with warfarin and development of new anticoagulants
- Normal reference value of orthodromic and antidromic sensory nerve conduction velocity of median nerve with intact palmaris longus tendon in apparently healthy individuals
- Short Communication
- Chemokine receptor antagonists with α1-adrenergic receptor blocker activity
