Phenolic-rich extracts of Eurycoma longifolia and Cylicodiscus gabunensis inhibit enzymes responsible for the development of erectile dysfunction and are antioxidants
-
Ganiyu Oboh
,
Adeniyi A. Adebayo
Abstract
Background:
Herbs have been used from ages to manage male sexual dysfunction. Hence, this study sought to investigate the effects of Eurycoma longifolia (EL) and Cylicodiscus gabunensis (CG) stem bark extracts on some enzymes implicated in erectile dysfunction in vitro.
Methods:
The extracts were prepared, and their effects on phosphodiesterase-5 (PDE-5), arginase, and angiotensin-1-converting enzyme (ACE) as well as pro-oxidant-induced lipid peroxidation were assessed. Furthermore, phenolic contents were determined, and their components were characterized and quantified using high-performance liquid chromatography with diode array detector (HPLC-DAD).
Results:
The results revealed that the extracts inhibited PDE-5, arginase, and ACE in a concentration-dependent manner. However, IC50 values revealed that CG had higher inhibitory potential on PDE-5 (IC50=204.4 μg/mL), arginase (IC50=39.01 μg/mL), and ACE (IC50=48.81 μg/mL) than EL. In addition, the extracts inhibited pro-oxidant-induced lipid peroxidation in penile tissue homogenate. HPLC-DAD analysis showed that CG is richer in phenolic compounds than EL, and this could be responsible for higher biological activities observed in CG than EL.
Conclusions:
Hence, the observed antioxidant property and inhibitory action of CG and EL on enzymes relevant to erectile dysfunction in vitro could be part of possible mechanisms underlying their involvement in traditional medicine for the management of male sexual dysfunction.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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©2018 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Review
- Pharmacotherapy of ulcerative colitis – current status and emerging trends
- Reproduction
- Bisphenol A decreases the spontaneous contractions of rat uterus in vitro through a nitrergic mechanism
- Cardiovascular Function
- An aqueous extract of the Anogeissus leiocarpus bark (AEAL) induces the endothelium-dependent relaxation of porcine coronary artery rings involving predominantly nitric oxide
- Oxidative Stress
- Alleviation of carbon tetrachloride-induced hepatocellular damage and oxidative stress with a leaf extract of Glyphae brevis (Tiliaceae)
- Protective effect of chloroform extract of Stereospermum chelonoides bark against amyloid beta42 induced cell death in SH-SY5Y cells and against inflammation in Swiss albino mice
- Metabolism
- Iodine in excess in the alterations of carbohydrate and lipid metabolic pattern as well as histomorphometric changes in associated organs
- Immune Response
- Screening of the immunomodulatory and antibacterial activity of Termitomyces letestui (Pat.) Heim (Lyophyllaceae), an edible mushroom from Cameroon
- Inflammation
- High-fat diet modifies cytokine gene expression and exacerbates the effects of acute pancreatitis in the liver of rats
- The acute anti-inflammatory action of xylopic acid isolated from Xylopia aethiopica
- Infection
- Prevalence and antibiotic utilization pattern of uropathogens causing community-acquired urinary tract infection in Kerala, India
- Phytotherapy
- Assessment of azadirachtin-A, a neem tetranortritarpinoid, on rat spermatozoa during in vitro capacitation
- Phenolic-rich extracts of Eurycoma longifolia and Cylicodiscus gabunensis inhibit enzymes responsible for the development of erectile dysfunction and are antioxidants
- Evaluation of anti-obesity and lipid-lowering properties of Vaccinium myrtillus leaves powder extract in a hamster model
Articles in the same Issue
- Frontmatter
- Review
- Pharmacotherapy of ulcerative colitis – current status and emerging trends
- Reproduction
- Bisphenol A decreases the spontaneous contractions of rat uterus in vitro through a nitrergic mechanism
- Cardiovascular Function
- An aqueous extract of the Anogeissus leiocarpus bark (AEAL) induces the endothelium-dependent relaxation of porcine coronary artery rings involving predominantly nitric oxide
- Oxidative Stress
- Alleviation of carbon tetrachloride-induced hepatocellular damage and oxidative stress with a leaf extract of Glyphae brevis (Tiliaceae)
- Protective effect of chloroform extract of Stereospermum chelonoides bark against amyloid beta42 induced cell death in SH-SY5Y cells and against inflammation in Swiss albino mice
- Metabolism
- Iodine in excess in the alterations of carbohydrate and lipid metabolic pattern as well as histomorphometric changes in associated organs
- Immune Response
- Screening of the immunomodulatory and antibacterial activity of Termitomyces letestui (Pat.) Heim (Lyophyllaceae), an edible mushroom from Cameroon
- Inflammation
- High-fat diet modifies cytokine gene expression and exacerbates the effects of acute pancreatitis in the liver of rats
- The acute anti-inflammatory action of xylopic acid isolated from Xylopia aethiopica
- Infection
- Prevalence and antibiotic utilization pattern of uropathogens causing community-acquired urinary tract infection in Kerala, India
- Phytotherapy
- Assessment of azadirachtin-A, a neem tetranortritarpinoid, on rat spermatozoa during in vitro capacitation
- Phenolic-rich extracts of Eurycoma longifolia and Cylicodiscus gabunensis inhibit enzymes responsible for the development of erectile dysfunction and are antioxidants
- Evaluation of anti-obesity and lipid-lowering properties of Vaccinium myrtillus leaves powder extract in a hamster model
