Bisphenol A decreases the spontaneous contractions of rat uterus in vitro through a nitrergic mechanism
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Hemlata Gupta
Abstract
Background:
Bisphenol A (BPA), a chemical used in the manufacture of plastics, has toxic effects on various systems of the human body including the reproductive system. BPA possesses estrogenic activity and is implicated in altering oogenesis, ovulation, and fertility. In addition to ovulatory changes, uterine contractility is an important factor for fertility. However, the effects of BPA on myometrial contractions are not known. Therefore, we examined the effect of BPA on rat uterine contractions.
Methods:
The uterus was isolated from adult rats showing estrous phase, and spontaneous in vitro contractions were recorded (35±1 °C). The effect of cumulative concentrations of BPA was determined. Further, the involvement of nitric oxide (NO) and guanylyl cyclase (GC) for the BPA-induced changes on uterine contractility was evaluated using the NO synthase inhibitor (L-NAME) or GC inhibitor (methylene blue).
Results:
BPA decreased the amplitude and frequency of spontaneous uterine contractions in a concentration-dependent manner. A decrease of 50% occurred at 1 and 3 μM for amplitude and frequency, respectively. L-NAME (N-ω-nitro-l-arginine methyl ester) blocked the BPA-induced decrease in amplitude at all concentrations but antagonized the frequency only at the maximum concentration (10 μM). Methylene blue (a GC inhibitor) did not block the BPA-induced responses but for the frequency at 10 μM of BPA.
Conclusions:
The results indicate that BPA decreased the amplitude and frequency of spontaneous uterine contractions by involving the nitrergic mechanism; however, the GC mechanism is not involved in the depression.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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©2018 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Review
- Pharmacotherapy of ulcerative colitis – current status and emerging trends
- Reproduction
- Bisphenol A decreases the spontaneous contractions of rat uterus in vitro through a nitrergic mechanism
- Cardiovascular Function
- An aqueous extract of the Anogeissus leiocarpus bark (AEAL) induces the endothelium-dependent relaxation of porcine coronary artery rings involving predominantly nitric oxide
- Oxidative Stress
- Alleviation of carbon tetrachloride-induced hepatocellular damage and oxidative stress with a leaf extract of Glyphae brevis (Tiliaceae)
- Protective effect of chloroform extract of Stereospermum chelonoides bark against amyloid beta42 induced cell death in SH-SY5Y cells and against inflammation in Swiss albino mice
- Metabolism
- Iodine in excess in the alterations of carbohydrate and lipid metabolic pattern as well as histomorphometric changes in associated organs
- Immune Response
- Screening of the immunomodulatory and antibacterial activity of Termitomyces letestui (Pat.) Heim (Lyophyllaceae), an edible mushroom from Cameroon
- Inflammation
- High-fat diet modifies cytokine gene expression and exacerbates the effects of acute pancreatitis in the liver of rats
- The acute anti-inflammatory action of xylopic acid isolated from Xylopia aethiopica
- Infection
- Prevalence and antibiotic utilization pattern of uropathogens causing community-acquired urinary tract infection in Kerala, India
- Phytotherapy
- Assessment of azadirachtin-A, a neem tetranortritarpinoid, on rat spermatozoa during in vitro capacitation
- Phenolic-rich extracts of Eurycoma longifolia and Cylicodiscus gabunensis inhibit enzymes responsible for the development of erectile dysfunction and are antioxidants
- Evaluation of anti-obesity and lipid-lowering properties of Vaccinium myrtillus leaves powder extract in a hamster model
Articles in the same Issue
- Frontmatter
- Review
- Pharmacotherapy of ulcerative colitis – current status and emerging trends
- Reproduction
- Bisphenol A decreases the spontaneous contractions of rat uterus in vitro through a nitrergic mechanism
- Cardiovascular Function
- An aqueous extract of the Anogeissus leiocarpus bark (AEAL) induces the endothelium-dependent relaxation of porcine coronary artery rings involving predominantly nitric oxide
- Oxidative Stress
- Alleviation of carbon tetrachloride-induced hepatocellular damage and oxidative stress with a leaf extract of Glyphae brevis (Tiliaceae)
- Protective effect of chloroform extract of Stereospermum chelonoides bark against amyloid beta42 induced cell death in SH-SY5Y cells and against inflammation in Swiss albino mice
- Metabolism
- Iodine in excess in the alterations of carbohydrate and lipid metabolic pattern as well as histomorphometric changes in associated organs
- Immune Response
- Screening of the immunomodulatory and antibacterial activity of Termitomyces letestui (Pat.) Heim (Lyophyllaceae), an edible mushroom from Cameroon
- Inflammation
- High-fat diet modifies cytokine gene expression and exacerbates the effects of acute pancreatitis in the liver of rats
- The acute anti-inflammatory action of xylopic acid isolated from Xylopia aethiopica
- Infection
- Prevalence and antibiotic utilization pattern of uropathogens causing community-acquired urinary tract infection in Kerala, India
- Phytotherapy
- Assessment of azadirachtin-A, a neem tetranortritarpinoid, on rat spermatozoa during in vitro capacitation
- Phenolic-rich extracts of Eurycoma longifolia and Cylicodiscus gabunensis inhibit enzymes responsible for the development of erectile dysfunction and are antioxidants
- Evaluation of anti-obesity and lipid-lowering properties of Vaccinium myrtillus leaves powder extract in a hamster model
