Silymarin prevents lipid accumulation in the liver of rats with type 2 diabetes via sirtuin1 and SREBP-1c
-
Nejat Kheiripour
,
Iraj Khodadadi
Abstract
Background:
In this study, we have investigated whether silymarin intake influences lipid and glycogen content in conjunction with sirtuin1 (SIRT1) and sterol regulatory element-binding protein 1c (SREBP-1c) expressions in liver of type 2 diabetic rat.
Methods:
Thirty-six male Wistar rats were randomly divided into six groups: control groups (C) and diabetic groups (D); the control groups received 60 or 120 mg/kg silymarin (C+S60 or C+S120), and the diabetic groups received 60 or 120 mg/kg silymarin (D+S60 or D+S120) daily for 8 weeks. Serum biochemical parameters, as well as glycogen, lipid and oxidative stress biomarkers, in the liver tissue were measured by spectrophotometric methods. Additionally, SIRT1 and SREBP-1c messenger RNA (mRNA) expressions were evaluated by quantitative polymerase chain reaction.
Results:
Diabetes caused a significantly increased fasting blood sugar, homeostasis model assessment for insulin resistance, liver total cholesterol and triglyceride (TG) content, which were attenuated after the administration of silymarin. Dietary silymarin caused the improvement of lipid content in the liver of diabetic rats. Moreover, silymarin administration promoted SIRT1, suppressed SREBP-1c mRNA expression, reduced liver nitric oxide and protein carbonyl content, and increased liver glycogen, catalase and glutathione peroxidase activity. Furthermore, histopathological changes were improved in the treated groups.
Conclusions:
Silymarin administration considerably restored hepatic changes induced by streptozotocin and nicotinamide. The upregulation of SIRT1 mRNA expression by silymarin may be associated with decreased lipid, increased glycogen content and downregulation of the SREBP-1c gene in the liver.
Acknowledgments
This study was financially supported by the Hamadan University of Medical Sciences (Funder Id: 10.13039/501100004697, No. 9505052617). The results presented in this article were part of N. Kheiripour’s Ph.D. thesis.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved the submission.
Research funding: Hamadan University of Medical Sciences (No. 9505052617).
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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©2018 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Review
- Calcium sensitization mechanisms in detrusor smooth muscles
- Opinion Paper
- The case for a distinctive philosophy of physiology and pathophysiology
- Behavior and Neuroprotection
- Effects of dizocilpine-induced glutamatergic blockade in the nucleus accumbens septi on the plus maze test
- Reproduction
- [6]-Gingerol modulates spermatotoxicity associated with ulcerative colitis and benzo[a]pyrene exposure in BALB/c mice
- Cardiovascular Function
- Blood troponin levels in acute cardiac events depends on space weather activity components (a correlative study)
- Effects of Trypanosoma brucei brucei infection and diminazene aceturate administration on the blood pressure, heart rate, and temperature of Wistar albino rats
- Oxidative Stress
- Dose-dependent effects of vitamin 1,25(OH)2D3 on oxidative stress and apoptosis
- Metabolism
- Losartan improves renal function and pathology in obese ZSF-1 rats
- Phytotherapy
- Evaluation of morning glory (Jacquemontia tamnifolia (L.) Griseb) leaves for antioxidant, antinociceptive, anticoagulant and cytotoxic activities
- Silymarin prevents lipid accumulation in the liver of rats with type 2 diabetes via sirtuin1 and SREBP-1c
Articles in the same Issue
- Frontmatter
- Review
- Calcium sensitization mechanisms in detrusor smooth muscles
- Opinion Paper
- The case for a distinctive philosophy of physiology and pathophysiology
- Behavior and Neuroprotection
- Effects of dizocilpine-induced glutamatergic blockade in the nucleus accumbens septi on the plus maze test
- Reproduction
- [6]-Gingerol modulates spermatotoxicity associated with ulcerative colitis and benzo[a]pyrene exposure in BALB/c mice
- Cardiovascular Function
- Blood troponin levels in acute cardiac events depends on space weather activity components (a correlative study)
- Effects of Trypanosoma brucei brucei infection and diminazene aceturate administration on the blood pressure, heart rate, and temperature of Wistar albino rats
- Oxidative Stress
- Dose-dependent effects of vitamin 1,25(OH)2D3 on oxidative stress and apoptosis
- Metabolism
- Losartan improves renal function and pathology in obese ZSF-1 rats
- Phytotherapy
- Evaluation of morning glory (Jacquemontia tamnifolia (L.) Griseb) leaves for antioxidant, antinociceptive, anticoagulant and cytotoxic activities
- Silymarin prevents lipid accumulation in the liver of rats with type 2 diabetes via sirtuin1 and SREBP-1c
