Dose-dependent effects of vitamin 1,25(OH)2D3 on oxidative stress and apoptosis
-
Cagri Cakici
,
Sule Ayla
Abstract
Background
The purpose of this study is to examine the dose-dependent effects of vitamin 1,25(OH)2D3 on apoptosis and oxidative stress.
Methods
In this study, 50 male Balb/c mice were used as control and experiment groups. The mice were divided into 5 groups each consisting of 10 mice. Calcitriol was intraperitoneally administered as low dose, medium dose, medium-high dose and high dose vitamin D groups (at 0.5, 1, 5 and 10 μg/kg, respectively), for three times a week during 14 days. At the end of the study, annexin V was measured by enzyme-linked immunosorbent assay method, and total antioxidant capacity and total oxidant status values were measured by colorimetric method in serum. Hematoxylin eosin staining was performed in liver tissues and periodic acid schiff staining was performed in kidney tissues.
Results
While comparing the results of medium-high dose (5 μg/kg) and high dose (10 μg/kg) vitamin D administration to that of the control group, it was observed that serum antioxidant status and annexin V levels decreased and glomerular mesenchial matrix ratio increased in kidney (p<0.05). In addition to these findings, in the group receiving high dose vitamin D (10 μg/kg), it was observed that the damage to the liver increased together with the the oxidative stress index values (p<0.05).
Conclusions
As a result, this study was the first in the literature to report that use of high-dose vitamin D (10 μg/kg) results in oxidant effect, rather than being an antioxidant, and causes severe histopathological toxicity in the liver and kidney.
Acknowledgments
We would like to special thank MSc. Ozge Biceroglu and MSc. Tugce Onel from Histology Department at the Medipol University who assisted with histopathological studies.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: This work was funded by a grant from the Istanbul Medipol University (Grant no. BAP-2016/17).
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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©2018 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Review
- Calcium sensitization mechanisms in detrusor smooth muscles
- Opinion Paper
- The case for a distinctive philosophy of physiology and pathophysiology
- Behavior and Neuroprotection
- Effects of dizocilpine-induced glutamatergic blockade in the nucleus accumbens septi on the plus maze test
- Reproduction
- [6]-Gingerol modulates spermatotoxicity associated with ulcerative colitis and benzo[a]pyrene exposure in BALB/c mice
- Cardiovascular Function
- Blood troponin levels in acute cardiac events depends on space weather activity components (a correlative study)
- Effects of Trypanosoma brucei brucei infection and diminazene aceturate administration on the blood pressure, heart rate, and temperature of Wistar albino rats
- Oxidative Stress
- Dose-dependent effects of vitamin 1,25(OH)2D3 on oxidative stress and apoptosis
- Metabolism
- Losartan improves renal function and pathology in obese ZSF-1 rats
- Phytotherapy
- Evaluation of morning glory (Jacquemontia tamnifolia (L.) Griseb) leaves for antioxidant, antinociceptive, anticoagulant and cytotoxic activities
- Silymarin prevents lipid accumulation in the liver of rats with type 2 diabetes via sirtuin1 and SREBP-1c
Articles in the same Issue
- Frontmatter
- Review
- Calcium sensitization mechanisms in detrusor smooth muscles
- Opinion Paper
- The case for a distinctive philosophy of physiology and pathophysiology
- Behavior and Neuroprotection
- Effects of dizocilpine-induced glutamatergic blockade in the nucleus accumbens septi on the plus maze test
- Reproduction
- [6]-Gingerol modulates spermatotoxicity associated with ulcerative colitis and benzo[a]pyrene exposure in BALB/c mice
- Cardiovascular Function
- Blood troponin levels in acute cardiac events depends on space weather activity components (a correlative study)
- Effects of Trypanosoma brucei brucei infection and diminazene aceturate administration on the blood pressure, heart rate, and temperature of Wistar albino rats
- Oxidative Stress
- Dose-dependent effects of vitamin 1,25(OH)2D3 on oxidative stress and apoptosis
- Metabolism
- Losartan improves renal function and pathology in obese ZSF-1 rats
- Phytotherapy
- Evaluation of morning glory (Jacquemontia tamnifolia (L.) Griseb) leaves for antioxidant, antinociceptive, anticoagulant and cytotoxic activities
- Silymarin prevents lipid accumulation in the liver of rats with type 2 diabetes via sirtuin1 and SREBP-1c
