Effects of a new chlorhexidine varnish on Streptococcus mutans biofilm formation in vitro
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Ali Hayati
Abstract
Background
Local sustained-release drug delivery systems increase the substantivity of drugs in the oral environment and subsequently enhance their therapeutic effects. This study sought to compare the effects of two commercially available varnishes and one experimental chlorhexidine (CHX) varnish on formation of Streptococcus mutans biofilm. The solubility rates of the varnishes were evaluated as well.
Methods
Standard acrylic discs were fabricated and divided into groups based on the varnish applied to the disc surfaces, namely, V-varnish, Pascal, and experimental CHX varnish. The effects of the varnishes on S. mutans biofilm were assessed after 48 h. Bacterial growth on the discs was evaluated by colony count and scanning electron microscopy (SEM). Solubility was assessed by immersing the samples in phosphate buffered saline and recording their weight changes at different times. The data were analyzed using one-way ANOVA.
Results
In the Pascal and experimental varnish groups, the total number of bacteria did not differ from that in the negative control group. The SEM findings confirmed the aforementioned results. Solubility varied significantly among the materials. V-varnish was detached from the surfaces after 2 days. No significant weight change was noted in the experimental varnish group at 14 days, while Pascal varnish showed gradual weight loss from the 5th day to the 10th day and had a plateau thereafter.
Conclusions
Biofilm formation was inhibited by the Pascal and experimental varnishes but not by the V-varnish. The highest acceptable rate of solubility was observed in the Pascal samples.
Acknowledgments
The authors are grateful to the Research Institute of Dental Sciences, School of Dentistry, Shahid Beheshti University of Medical Sciences for financial support. The authors thank R. Shahbazi for her skilled assistance.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: Research Institute of Dental Sciences, School of Dentistry, Shahid Beheshti University of Medical Sciences.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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©2018 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
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- Orphan drugs: trends and issues in drug development
- Phytotherapy
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- Garlic (Allium sativum) increases SIRT1 and SIRT2 gene expressions in the kidney and liver tissues of STZ- and STZ+niacinamide-induced diabetic rats
- Reproduction
- A comparative study of the effect of stress on the cognitive parameters in women with increased body mass index before and after menopause
- Comparative effect of the aqueous extracts of Aloe buettneri, Dicliptera verticillata, Hibiscus macranthus and Justicia insularis on the sexual maturation of pregnant mare serum gonadotrophin-primed immature female rats
- Oxidative Stress
- Effect of L-carnitine on the skeletal muscle contractility in simvastatin-induced myopathy in rats
- Infection
- Anti-plasmodial activity of sodium acetate in Plasmodium berghei-infected mice
- Vascular Conditions
- Role of atrial natriuretic peptide in controlling diabetic nephropathy in rats
- Inflammation
- Anti-inflammatory and insulin secretory activity in experimental type-2 diabetic rats treated orally with magnesium
- Effects of artemisinin, with or without lumefantrine and amodiaquine on gastric ulcer healing in rat
- Behavior and Neuroprotection
- Effect of flavonol and its dimethoxy derivatives on paclitaxel-induced peripheral neuropathy in mice
- Hematological Profile
- Interactive effects of alcohol and chloroquine on hematologic profile of Wistar rats
- Metabolism
- Effects of α-(prazosin and yohimbine) and β-receptors activity on cAMP generation and UCP1 gene expression in brown adipocytes
- Differential sensitivity of chronic high-fat-diet-induced obesity in Sprague-Dawley rats
- Cardiovascular-Pulmonary Interactions
- A pilot study exploring the impact of cardiac medications on ciliary beat frequency: possible implications for clinical management
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Articles in the same Issue
- Frontmatter
- Review
- Rabbits and men: relating their ages
- Minireview
- Orphan drugs: trends and issues in drug development
- Phytotherapy
- Influence of Loranthus micranthus on hepatic and renal antioxidant status and impaired glycolytic flux in streptozotocin-induced diabetic rats
- Garlic (Allium sativum) increases SIRT1 and SIRT2 gene expressions in the kidney and liver tissues of STZ- and STZ+niacinamide-induced diabetic rats
- Reproduction
- A comparative study of the effect of stress on the cognitive parameters in women with increased body mass index before and after menopause
- Comparative effect of the aqueous extracts of Aloe buettneri, Dicliptera verticillata, Hibiscus macranthus and Justicia insularis on the sexual maturation of pregnant mare serum gonadotrophin-primed immature female rats
- Oxidative Stress
- Effect of L-carnitine on the skeletal muscle contractility in simvastatin-induced myopathy in rats
- Infection
- Anti-plasmodial activity of sodium acetate in Plasmodium berghei-infected mice
- Vascular Conditions
- Role of atrial natriuretic peptide in controlling diabetic nephropathy in rats
- Inflammation
- Anti-inflammatory and insulin secretory activity in experimental type-2 diabetic rats treated orally with magnesium
- Effects of artemisinin, with or without lumefantrine and amodiaquine on gastric ulcer healing in rat
- Behavior and Neuroprotection
- Effect of flavonol and its dimethoxy derivatives on paclitaxel-induced peripheral neuropathy in mice
- Hematological Profile
- Interactive effects of alcohol and chloroquine on hematologic profile of Wistar rats
- Metabolism
- Effects of α-(prazosin and yohimbine) and β-receptors activity on cAMP generation and UCP1 gene expression in brown adipocytes
- Differential sensitivity of chronic high-fat-diet-induced obesity in Sprague-Dawley rats
- Cardiovascular-Pulmonary Interactions
- A pilot study exploring the impact of cardiac medications on ciliary beat frequency: possible implications for clinical management
- Genotoxicity and Cytotoxicity
- Effects of a new chlorhexidine varnish on Streptococcus mutans biofilm formation in vitro
