Evaluation of oral multi-herbal preparation of Dashmoolarishta on mice model of osteoarthritis
-
Yashashri C. Shetty
,
Santosh Godbharle
Abstract
Background:
Osteoarthritis (OA) is a chronic progressive disease commonly affecting the hip and knee joints. Although synthetic drugs are available and afford symptomatic relief, their side effects pose limitations to their continuous use. So, this research was focused on extracting drugs from indigenous medicinal plants that could have a beneficial effect on osteoarthritis. Dashmoolarishta is one such preparation whose effects have never been studied in comparison with recent drugs like hyaluronic acid (HA), hence this particular study was undertaken. The aim of this study was to evaluate the effects of Dashmoolarishta compared with HA on joint pathology and pain behavior in monosodiumiodoacetate (MIA)-induced OA in experimental mice.
Methods:
The study was initiated after obtaining permission from the Animal Ethics Committee. This study was based on the MIA model of osteoarthritis, with mice being divided into five groups viz.: disease control (DC), Dasahmoolarishta high dose (HD) and low dose (LD), sham control (SC) and HA. The OA of the knee joint was induced in these mice using monosodiumiodoacetate. Seven days after induction, animals were subjected to weekly behavioral tests, daily oral Dashmoolarishta, and biweekly HA administration from weeks 2–4. At the end of the 4th week, histopathological examination of the knee joints was done.
Results:
DC showed significant osteoarthritic changes. At week 4, the behavioral tests and histopathology results of all groups were found to be significant. A significant difference (p<0.05) was found between DC vs. SC, HA, HD, LD for open field test, Rota rod test, knee joint diameter, and Cat walk test. Dashmoolarishta HD and LD showed significant improvement in pain, as assessed by behavioral tests (p<0.05) and pathology, as assessed by knee joint histopathology (p<0.05).
Conclusions:
Oral Dashmoolarishta showed reduction in pain and disease activity in MIA-induced osteoarthritis in mice model.
Acknowledgments
Authors acknowledge the Sandu Pharmaceuticals Ltd, Mumbai for providing the drugs for this study and the Diamond Jubilee Society Trust for funding the study.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: Research funded by Diamond Jubilee Society Trust, Seth G S Medical College and KEM Hospital, Mumbai.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretationof data; in the writing of the report; or in the decision to submit the report for publication.
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©2017 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Editorial
- Inflammation, fever, and body temperature under febrile conditions
- Inflammation
- Anti-inflammatory effects and anti-oxidant capacity of Myrathius arboreus (Cecropiaceae) in experimental models
- Ocimum gratissimum Linn. Leaf extract inhibits free radical generation and suppressed inflammation in carrageenan-induced inflammation models in rats
- Intra-articular injections of ketamine and 25% dextrose improve clinical and pathological outcomes in the monosodium iodoacetate model of osteoarthritis
- Central mediators of the zymosan-induced febrile response
- The use of siRNA as a pharmacological tool to assess a role for the transcription factor NF-IL6 in the brain under in vitro and in vivo conditions during LPS-induced inflammatory stimulation
- Effects of single or combined administration of salmon calcitonin and omega-3 fatty acids vs. diclofenac sodium in sodium monoiodoacetate-induced knee osteoarthritis in male Wistar rats
- Evaluation of oral multi-herbal preparation of Dashmoolarishta on mice model of osteoarthritis
- Behavior and Neuroprotection
- Possible modulation of PPAR-γ cascade against depression caused by neuropathic pain in rats
- Functional interaction between N-methyl-D-aspartate receptor and ascorbic acid during neuropathic pain induced by chronic constriction injury of the sciatic nerve
- Cardiovascular Function
- Chronic endurance exercise antagonizes the cardiac UCP2 and UCP3 protein up-regulation induced by nandrolone decanoate
- Oxidative Stress
- Prevention of renal ischemia/perfusion-induced renal and hepatic injury in adult male Albino rats by oxytocin: role of nitric oxide
- Protective effect of salusin-α and salusin-β against ethanol-induced gastric ulcer in rats
Artikel in diesem Heft
- Frontmatter
- Editorial
- Inflammation, fever, and body temperature under febrile conditions
- Inflammation
- Anti-inflammatory effects and anti-oxidant capacity of Myrathius arboreus (Cecropiaceae) in experimental models
- Ocimum gratissimum Linn. Leaf extract inhibits free radical generation and suppressed inflammation in carrageenan-induced inflammation models in rats
- Intra-articular injections of ketamine and 25% dextrose improve clinical and pathological outcomes in the monosodium iodoacetate model of osteoarthritis
- Central mediators of the zymosan-induced febrile response
- The use of siRNA as a pharmacological tool to assess a role for the transcription factor NF-IL6 in the brain under in vitro and in vivo conditions during LPS-induced inflammatory stimulation
- Effects of single or combined administration of salmon calcitonin and omega-3 fatty acids vs. diclofenac sodium in sodium monoiodoacetate-induced knee osteoarthritis in male Wistar rats
- Evaluation of oral multi-herbal preparation of Dashmoolarishta on mice model of osteoarthritis
- Behavior and Neuroprotection
- Possible modulation of PPAR-γ cascade against depression caused by neuropathic pain in rats
- Functional interaction between N-methyl-D-aspartate receptor and ascorbic acid during neuropathic pain induced by chronic constriction injury of the sciatic nerve
- Cardiovascular Function
- Chronic endurance exercise antagonizes the cardiac UCP2 and UCP3 protein up-regulation induced by nandrolone decanoate
- Oxidative Stress
- Prevention of renal ischemia/perfusion-induced renal and hepatic injury in adult male Albino rats by oxytocin: role of nitric oxide
- Protective effect of salusin-α and salusin-β against ethanol-induced gastric ulcer in rats
