Long-term exposure to a butter-rich diet induces mild-to-moderate steatosis in Chang liver cells and Swiss albino mice models
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Thomas John Philip Nalloor
Abstract
Background:
Butter is one of the widely used fats present in the diet. However, there is no satisfactory study available that evaluates the effect of a high-fat diet containing butter as the principal fat on the development of non-alcoholic fatty liver disease (NAFLD).
Methods:
In the present study, butter was used for the development of steatosis in Chang liver cells in an in vitro study and Swiss albino mice in an in vivo study. In vitro steatosis was established, and butter was compared with oleic acid in Chang liver cells using an oil red O (ORO)-based colorimetric assay. In the in vivo study, a butter-rich special diet was fed for 15 weeks to mice, who showed no significant change in body weight. The expression pattern of phosphatase and tensin homolog (PTEN) and miR-21 was compared by reverse transcriptase-PCR.
Results and Conclusions:
Special diet-fed animals showed downregulated PTEN compared to normal diet-fed animals, while levels of miR-21 remained the same. Elevations in biochemical parameters, viz., triglycerides and liver function tests showed symptoms of onset of NAFLD. Histophathological study of livers of test animals confirmed mild-to-moderate degree of NAFLD.
Acknowledgments
We would like to thank the Manipal College of Pharmaceutical Sciences, Manipal, and Manipal University for providing the research facility. The financial support from DST-FIST is gratefully acknowledged.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: Department of Science and Technology, Ministry of Science and Technology.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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©2017 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Review
- Isolated heart models for studying cardiac electrophysiology: a historical perspective and recent advances
- Reproduction
- Sub-chronic indomethacin treatment and its effect on the male reproductive system of albino rats: possible protective role of black tea extract
- The effect of extracellular ATP on rat uterine contraction from different gestational stages and its possible mechanisms of action
- Cardiovascular Function
- Effect of cardiovascular and muscular endurance is not associated with stress fracture incidence in female military recruits: a 12-month follow up study
- Cardiovascular-Pulmonary Interactions
- Comparison of structured and unstructured physical activity training on predicted VO2max and heart rate variability in adolescents – a randomized control trial
- Oxidative Stress
- Aminoguanidine pretreatment prevents methotrexate-induced small intestinal injury in the rat by attenuating nitrosative stress and restoring the activities of vital mitochondrial enzymes
- Anti-aging effects of M2000 (β-D-mannuronic acid) as a novel immunosuppressive drug on the enzymatic and non-enzymatic oxidative stress parameters in an experimental model
- Metabolism
- Long-term exposure to a butter-rich diet induces mild-to-moderate steatosis in Chang liver cells and Swiss albino mice models
- Effect of adrenocorticotropic hormone on UCP1 gene expression in brown adipocytes
- Phytotherapy
- Antidiabetic effect of Ruta montana L. in streptozotocin-induced diabetic rats
- Ethnopharmacological investigation of the aerial part of Phragmites karka (Poaceae)
Artikel in diesem Heft
- Frontmatter
- Review
- Isolated heart models for studying cardiac electrophysiology: a historical perspective and recent advances
- Reproduction
- Sub-chronic indomethacin treatment and its effect on the male reproductive system of albino rats: possible protective role of black tea extract
- The effect of extracellular ATP on rat uterine contraction from different gestational stages and its possible mechanisms of action
- Cardiovascular Function
- Effect of cardiovascular and muscular endurance is not associated with stress fracture incidence in female military recruits: a 12-month follow up study
- Cardiovascular-Pulmonary Interactions
- Comparison of structured and unstructured physical activity training on predicted VO2max and heart rate variability in adolescents – a randomized control trial
- Oxidative Stress
- Aminoguanidine pretreatment prevents methotrexate-induced small intestinal injury in the rat by attenuating nitrosative stress and restoring the activities of vital mitochondrial enzymes
- Anti-aging effects of M2000 (β-D-mannuronic acid) as a novel immunosuppressive drug on the enzymatic and non-enzymatic oxidative stress parameters in an experimental model
- Metabolism
- Long-term exposure to a butter-rich diet induces mild-to-moderate steatosis in Chang liver cells and Swiss albino mice models
- Effect of adrenocorticotropic hormone on UCP1 gene expression in brown adipocytes
- Phytotherapy
- Antidiabetic effect of Ruta montana L. in streptozotocin-induced diabetic rats
- Ethnopharmacological investigation of the aerial part of Phragmites karka (Poaceae)
