Kolaviron, a biflavonoid fraction from Garcinia kola, protects against isoproterenol-induced injury by mitigating cardiac dysfunction and oxidative stress in rats
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Oluwatosin Adekunle Adaramoye
und
Semiu Olasoju Lawal
Abstract
Background: The aim of this study was to evaluate the cardioprotective effects of kolaviron (KV), a biflavonoid from Garcinia kola seeds, in rats intoxicated with isoproterenol chloride (ISO) while quercetin (QUE) served as standard.
Methods: Forty-two male Wistar rats (180–200 g) were randomly divided into seven groups of six rats each. Group 1 served as control; group 2 received ISO (85 mg/kg subcutaneously); groups 3, 4 and 5 received ISO and KV1 [100 mg/kg orally (p.o.)], KV2 (200 mg/kg, p.o.) and QUE (25 mg/kg, p.o.), respectively; and groups 6 and 7 received QUE and KV2, respectively.
Results: Administration of ISO caused significant (p<0.05) elevation of serum creatine phosphokinase, lactate dehydrogenase, alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase by 2.2-, 1.9-, 2.1-, 1.9- and 1.7-fold, respectively, relative to controls, with a concomitant decrease in cardiac activities of these enzymes. Administration of ISO led to significant decrease (p<0.05) in the levels of cardiac superoxide dismutase, catalase, glutathione S-transferase, reduced glutathione and increase in malondialdehyde (MDA). Also, ISO-treated rats had significantly higher values of serum total cholesterol, triglycerides and low-density lipoprotein cholesterol relative to controls. Supplementation with KV2 and QUE caused significant elevation of cardiac antioxidant enzymes, normalized the marker enzymes and reduced MDA levels.
Conclusions: KV protects against ISO-induced cardiotoxicity in vivo, suggesting its usefulness as a possible chemoprophylactic agent against cardiotoxic drugs.
Acknowledgments
This research was partly supported by Senate Research Grants from University of Ibadan given to OAA (SRG/COM/2010/7A). Isoproterenol chloride was a gift from the laboratory of Dr. Hagen Wolf Schunck of Max Delbrück Center of Molecular Medicine, 13125 Berlin-Buch, Germany.
Conflict of interest statement
Authors’ conflict of interest disclosure: The authors stated that there are no conflicts of interest regarding the publication of this article. Research support played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
References
1. Arima H, Barzi F, Chalmers J. Mortality patterns in hypertension. J Hypertens 2011;29:S3–7.10.1097/01.hjh.0000410246.59221.b1Suche in Google Scholar PubMed
2. Siddiqui AM, Samad Z, Crowley AL, Hakacova N, Harrison JK, Wagner GS. Relationships between cardiac magnetic resonance imaging abnormalities in the inter-ventricular septum and Selvester QRS scoring criteria for anterior-septal myocardial infarction in patients with right ventricular volume overload. J Electrocardiol 2013;46:256–62.10.1016/j.jelectrocard.2013.02.010Suche in Google Scholar PubMed
3. Gruzdeva O, Uchasova E, Dyleva Y, Belik E, Kashtalap V, Barbarash O. Relationship between free fatty acids, insulin resistance markers, and oxidized lipoproteins in myocardial infarction and acute left ventricular failure. Diabetes Metab Syndr Obes 2013;6:103–11.Suche in Google Scholar
4. Vazzana N, Ganci A, Cefalù AB, Lattanzio S, Noto D, Santoro N, et al. Enhanced lipid peroxidation and platelet activation as potential contributors to increased cardiovascular risk in the low-HDL phenotype. J Am Heart Assoc 2013;2:e000063.10.1161/JAHA.113.000063Suche in Google Scholar PubMed PubMed Central
5. Nichtova Z, Novotova M, Kralova E, Stankovicova T. Morphological and functional characteristics of models of experimental myocardial injury induced by isoproterenol. Gen Physiol Biophys 2012;31:141–51.10.4149/gpb_2012_015Suche in Google Scholar PubMed
6. Takaki M. Cardiac mechanoenergetics for understanding isoproterenol-induced rat heart failure. Pathophysiology 2012;19:163–70.10.1016/j.pathophys.2012.04.004Suche in Google Scholar PubMed
7. Li X, Wang X, Guo Y, Deng N, Zheng P, Xu Q, et al. Regulation of endothelial nitric oxide synthase and asymmetric dimethylarginine by matrine attenuates isoproterenol-induced acute myocardial injury in rats. J Pharm Pharmacol 2012;64:1107–18.10.1111/j.2042-7158.2012.01502.xSuche in Google Scholar PubMed
8. Kannan MM, Quine SD. Ellagic acid inhibits cardiac arrhythmias, hypertrophy and hyperlipidaemia during myocardial infarction in rats. Metabolism 2013;62:52–61.10.1016/j.metabol.2012.06.003Suche in Google Scholar PubMed
9. Hutchinson J, Dalziel JM. Cycadaceae: guttiferae. In: Happer FN, editor. Flora of West Tropical Africa, vol. 11, 2nd ed. London: Her Majesty’s Stationary Office, 1956:295–7.Suche in Google Scholar
10. Iwu MM, Igboko OA. Flavonoids of Garcinia kola seeds. J Nat Prod 1982;45:650–1.10.1021/np50023a026Suche in Google Scholar
11. Farombi EO, Adedara IA, Ajayi BO, Ayepola OR, Egbeme EE. Kolaviron, a natural antioxidant and anti-inflammatory phytochemical prevents dextran sulphate sodium-induced colitis in rats. Basic Clin Pharmacol Toxicol 2013;113:49–55.10.1111/bcpt.12050Suche in Google Scholar PubMed
12. Adaramoye O. Antidiabetic effect of kolaviron, a biflavonoid complex isolated from Garcinia kola seeds, in Wistar rats. Afr Health Sci 2012;12:498–506.Suche in Google Scholar
13. Adaramoye OA, Nwaneri VO, Anyanwu KC, Farombi EO, Emerole GO. Possible anti-atherogenic effect of kolaviron (a Garcinia kola seed extract) in hypercholesterolemic rats. Clin Exp Pharmacol Physiol 2005;32:40–6.10.1111/j.1440-1681.2005.04146.xSuche in Google Scholar PubMed
14. Adaramoye OA, Medeiros IA. Endothelium-independent vasodilation induced by kolaviron, a biflavonoid complex from Garcinia kola seeds, in rat superior mesenteric arteries. J Smooth Muscle Res 2009;45:39–53.10.1540/jsmr.45.39Suche in Google Scholar PubMed
15. Nagai M, Conney AH, Zhu BT. Strong inhibitory effects of common tea catechins and bioflavonoids on the O-methylation of catechol estrogens catalyzed by human liver cytosolic catechol-O-methyltransferase. Drug Metab Dispos 2004;32:497–504.10.1124/dmd.32.5.497Suche in Google Scholar PubMed
16. Sharmila G, Athirai T, Kiruthiga B, Senthilkumar K, Elumalai P, Arunkumar R, et al. Chemopreventive effect of quercetin in MNU and testosterone induced prostate cancer of Sprague-Dawley rats. Nutr Cancer 2013, PMID: 24320139.10.1080/01635581.2014.847967Suche in Google Scholar PubMed
17. Aldemir M, Okulu E, Kösemehmetoğlu K, Ener K, Topal F, Evirgen O, et al. Evaluation of the protective effect of quercetin against cisplatin-induced renal and testis tissue damage and sperm parameters in rats. Andrologia 2013, DOI: 10.1111/and.12197.10.1111/and.12197Suche in Google Scholar PubMed
18. Adaramoye OA, Adedara IA, Farombi EO. Possible ameliorative effects of kolaviron against reproductive toxicity in sub-lethally whole body gamma-irradiated rats. Exp Toxicol Pathol 2012;64:379–85.10.1016/j.etp.2010.10.002Suche in Google Scholar PubMed
19. Adaramoye OA, Awogbindin I, Okusaga JO. Effect of kolaviron, a biflavonoid complex from Garcinia kola seeds, on ethanol-induced oxidative stress in liver of adult wistar rats. J Med Food 2009;12:584–90.10.1089/jmf.2008.0138Suche in Google Scholar PubMed
20. Saravanan R, Shanmugam A. Preventive effect of low molecular weight glycosaminoglycan from Amussium pleuronectus (Linne) on oxidative injury and cellular abnormalities in isoproterenol-induced cardiotoxicity in Wistar rats. Appl Biochem Biotechnol 2010;162:43–51.10.1007/s12010-009-8750-5Suche in Google Scholar PubMed
21. Liu CM, Zheng GH, Ming QL, Sun JM, Cheng C. Protective effect of quercetin on lead-induced oxidative stress and endoplasmic reticulum stress in rat liver via the IRE1/JNK and PI3K/Akt pathway. Free Radic Res 2013;47:192–201.10.3109/10715762.2012.760198Suche in Google Scholar PubMed
22. Adaramoye OA, Osaimoje DO, Akinsanya AM, Nneji CM, Fafunso MA, Ademowo OG. Changes in antioxidant status and biochemical indices after acute administration of artemether, artemether-lumefantrine and halofantrine in rats. Basic Clin Pharmacol Toxicol 2008;102:412–8.10.1111/j.1742-7843.2008.00211.xSuche in Google Scholar PubMed
23. Iskusnykh IY, Popova TN, Agarkov AA, Pinheiro de Carvalho MÂ, Rjevskiy SG. Expression of glutathione peroxidase and glutathione reductase and level of free radical processes under toxic hepatitis in rats. J Toxicol 2013; DOI: 10.1155/2013/870628.10.1155/2013/870628Suche in Google Scholar PubMed PubMed Central
24. Sathishkumar K, Gao X, Raghavamenon AC, Murthy SN, Kadowitz PJ, Uppu RM. Determination of glutathione, mitochondrial transmembrane potential, and cytotoxicity in H9c2 cardiomyoblasts exposed to reactive oxygen and nitrogen species. Methods Mol Biol 2010;610:51–61.10.1007/978-1-60327-029-8_4Suche in Google Scholar PubMed
25. Georgieva S, Popov B, Bonev G. Radioprotective effect of Haberlea rhodopensis (Friv.) leaf extract on gamma-radiation-induced DNA damage, lipid peroxidation and antioxidant levels in rabbit blood. Indian J Exp Biol 2013;51:29–36.Suche in Google Scholar
26. Gupta S, Verma M, Singh K. Does LDL-C estimation using Anandaraja’s formula give a better agreement with direct LDL-C estimation than the Friedewald’s formula? Indian J Clin Biochem 2012;27:127–33.10.1007/s12291-011-0186-3Suche in Google Scholar PubMed PubMed Central
27. Gayathri K, Jayachandran KS, Vasanthi HR, Rajamanickam GV. Cardioprotective effect of lemon grass as evidenced by biochemical and histopathological changes in experimentally induced cardiotoxicity. Hum Exp Toxicol 2011;30:1073–82.10.1177/0960327110386391Suche in Google Scholar PubMed
28. Panda S, Kar A, Sharma P, Sharma A. Cardioprotective potential of N,α-l-rhamnopyranosyl vincosamide, an indole alkaloid, isolated from the leaves of Moringa oleifera in isoproterenol induced cardiotoxic rats: in vivo and in vitro studies. Bioorg Med Chem Lett 2013;23:959–62.10.1016/j.bmcl.2012.12.060Suche in Google Scholar PubMed
29. Ojha S, Bharti S, Golechha M, Sharma AK, Rani N, Kumari S, et al. Andrographis paniculata extract protects against isoproterenol-induced myocardial injury by mitigating cardiac dysfunction and oxidative injury in rats. Acta Pol Pharm 2012;69:269–78.Suche in Google Scholar
30. Mohamadin AM, Elberry AA, Mariee AD, Morsy GM, Al-Abbasi FA. Lycopene attenuates oxidative stress and heart lysosomal damage in isoproterenol induced cardiotoxicity in rats: a biochemical study. Pathophysiology 2012;19:121–30.10.1016/j.pathophys.2012.04.005Suche in Google Scholar PubMed
31. Jancova P, Anzenbacher P, Anzenbacherova E. Phase II drug metabolizing enzymes. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 2010;154:103–16.10.5507/bp.2010.017Suche in Google Scholar PubMed
32. Abdel Salam OM, Mohammed NA, Sleem AA, Farrag AR. The effect of antidepressant drugs on thioacetamide-induced oxidative stress. Eur Rev Med Pharmacol Sci 2013; 17:735–44.Suche in Google Scholar
33. Kaplan KA, Odabasoglu F, Halici Z, Cadirci E, Atalay F, Aydin O, et al. Alpha-lipoic acid protects against indomethacin-induced gastric oxidative toxicity by modulating antioxidant system. J Food Sci 2012;77:H224–30.10.1111/j.1750-3841.2012.02920.xSuche in Google Scholar PubMed
34. Arslan M, Melek M, Demir H, Eseoglu M, Gudu BO, Demir I, et al. Relationship of antioxidant enzyme activities with myelomeningocele. Turk Neurosurg 2012;22:300–4.Suche in Google Scholar
35. László A, Novák Z, Szollosi-Varga I, Hai du Q, Vetró Á, Kovács A. Blood lipid peroxidation, antioxidant enzyme activities and hemorheological changes in autistic children. Ideggyogy Sz 2013;66:23–8.Suche in Google Scholar
36. Gümral N, Doguc Kumbul D, Aylak F, Saygin M, Savik E. Juniperus communis Linn oil decreases oxidative stress and increases antioxidant enzymes in the heart of rats administered a diet rich in cholesterol. Toxicol Ind Health 2013; DOI: 10.1177/0748233712469995.10.1177/0748233712469995Suche in Google Scholar PubMed
37. Lalitha G, Poornima P, Archanah A, Padma VV. protective effect of neferine against isoproterenol-induced cardiac toxicity. Cardiovasc Toxicol 2012;13:168–79.10.1007/s12012-012-9196-5Suche in Google Scholar PubMed
38. Zhou R, Xu Q, Zheng P, Yan L, Zheng J, Dai G. Cardioprotective effect of fluvastatin on isoproterenol-induced myocardial infarction in rat. Eur J Pharmacol 2008;586:244–50.10.1016/j.ejphar.2008.02.057Suche in Google Scholar PubMed
39. Mishra V, Ghumatkar P, Patel MV, Devada S, Ranvir R, Swain P, et al. Evaluation of Cardioprotective effect of 3,5,3′-tri-iodo-l-thyronine in isoproterenol-induced cardiotoxicity. Int J Endocrinol 2011;2011:908367.10.1155/2011/908367Suche in Google Scholar PubMed PubMed Central
40. Roy SJ, Mainzen Prince PS. Protective effects of sinapic acid on cardiac hypertrophy, dyslipidaemia and altered electrocardiogram in isoproterenol-induced myocardial infarcted rats. Eur J Pharmacol 2013;699:213–8.10.1016/j.ejphar.2012.11.012Suche in Google Scholar PubMed
41. Naghii MR, Almadadi M, Zarchi AA. Regular physical activity as a basic component of lifestyle modification reduces major cardiovascular risk factors among male armored force personnel of Shabestar army installation in Iran. Work 2011;40:217–27.10.3233/WOR-2011-1222Suche in Google Scholar PubMed
©2015 by De Gruyter
Artikel in diesem Heft
- Frontmatter
- Review
- Cell cycle checkpoints and pathogenesis of HIV-1 infection: a brief overview
- Behavior/Neuroprotection
- Neuroprotective effect of N-acetyl cysteine against streptozotocin-induced memory dysfunction and oxidative damage in rats
- Effect of (4a) a novel 5-HT3 receptor antagonist on chronic unpredictable mild stress induced depressive-like behavior in mice: an approach using behavioral tests battery
- Involvement of opioidergic and serotonergic systems in the analgesic activity of Cissus quadrangularis L. stem extract in mice
- Multiparameter rodent chronic model for complex evaluation of alcoholism-mediated metabolic violations
- Reproduction
- The methanol-soluble fraction of Millettia macrophylla (Fabaceae) stem bark endowed with estrogenic properties has adverse effects on the male reproductive system of Wistar rats
- Cardiovascular Function
- Kolaviron, a biflavonoid fraction from Garcinia kola, protects against isoproterenol-induced injury by mitigating cardiac dysfunction and oxidative stress in rats
- A comparative study to evaluate the cardiovascular risk of selective and nonselective cyclooxygenase inhibitors (COX-Is) in arthritic patients
- Metabolism
- Increased levels of serum γ-glutamyltransferase and uric acid on metabolic, hepatic and kidney parameters in subjects at high altitudes
- Phytotherapy
- Anti-inflammatory and antinociceptive activities of the aqueous leaf extract of Phyllanthus amarus Schum (Euphorbiaceae) in some laboratory animals
- Gastroprotective activity of essential oils from turmeric and ginger
- Decreased metabolism of 13C-caffeine via hepatic CYP1A2 in marasmus and kwashiorkor based on breath test
Artikel in diesem Heft
- Frontmatter
- Review
- Cell cycle checkpoints and pathogenesis of HIV-1 infection: a brief overview
- Behavior/Neuroprotection
- Neuroprotective effect of N-acetyl cysteine against streptozotocin-induced memory dysfunction and oxidative damage in rats
- Effect of (4a) a novel 5-HT3 receptor antagonist on chronic unpredictable mild stress induced depressive-like behavior in mice: an approach using behavioral tests battery
- Involvement of opioidergic and serotonergic systems in the analgesic activity of Cissus quadrangularis L. stem extract in mice
- Multiparameter rodent chronic model for complex evaluation of alcoholism-mediated metabolic violations
- Reproduction
- The methanol-soluble fraction of Millettia macrophylla (Fabaceae) stem bark endowed with estrogenic properties has adverse effects on the male reproductive system of Wistar rats
- Cardiovascular Function
- Kolaviron, a biflavonoid fraction from Garcinia kola, protects against isoproterenol-induced injury by mitigating cardiac dysfunction and oxidative stress in rats
- A comparative study to evaluate the cardiovascular risk of selective and nonselective cyclooxygenase inhibitors (COX-Is) in arthritic patients
- Metabolism
- Increased levels of serum γ-glutamyltransferase and uric acid on metabolic, hepatic and kidney parameters in subjects at high altitudes
- Phytotherapy
- Anti-inflammatory and antinociceptive activities of the aqueous leaf extract of Phyllanthus amarus Schum (Euphorbiaceae) in some laboratory animals
- Gastroprotective activity of essential oils from turmeric and ginger
- Decreased metabolism of 13C-caffeine via hepatic CYP1A2 in marasmus and kwashiorkor based on breath test
