Anti-inflammatory and antinociceptive activities of the aqueous leaf extract of Phyllanthus amarus Schum (Euphorbiaceae) in some laboratory animals
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Adeolu A. Adedapo
und
Sunday O. Ofuegbe
Abstract
Background: Phyllanthus amarus has a history of use in Ayurvedic medicine for over 2000 years as well as a wide variety of traditional applications and has gained popularity in many continents as a herbal remedy; hence, it is being assessed for its safety potential and anti-inflammatory and analgesic properties in some laboratory animals.
Methods: Standard phytochemical methods were used to test for the presence of phytoactive compounds in the plant. Acute toxicity testing was carried out in mice to determine safe doses for the extract. The anti-inflammatory activity of the leaf extract of this plant was assessed using carrageenan-induced and histamine-induced paw edema. The analgesic effect was determined using the acetic acid writhing method as well as formalin test.
Results: The extract at 100 and 200 mg/kg body weight reduced significantly, the formation of edema induced by carrageenan and histamine. In the acetic acid-induced writhing model, the extract showed a good analgesic effect characterized by reduction in the number of writhes when compared to the control. The extract caused dose-dependent decrease of licking time in rats injected with 2.5% formalin, signifying its analgesic effect. These results were also comparable to those of ibuprofen, the reference drug used in this study.
Conclusions: The plant extract reduced significantly the formation of edema induced by carrageenan and histamine as well as reducing the number of writhes in acetic acid-induced writhing models and dose-dependent decrease of licking time in rats injected with 2.5% formalin. The results have validated the basis for the traditional use of P. amarus as a medicinal plant.
Acknowledgments
The authors wish to acknowledge the financial support of the University of Ibadan Senate Research Grant (SRG/FVM/2010/10A) in carrying out this study.
Conflict of interest statement
Authors’ conflict of interest disclosure: The authors stated that there are no conflicts of interest regarding the publication of this article. Research support played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
References
1. Sobota R, Szwed M, Kasza A, Bugno M, Kordula T. Parthenolide inhibits activation of signal transducers and activators of transcription (STATs) induced by cytokines of the IL-6 family. Biochem Biophys Res Commun 2000;267:329–33.10.1006/bbrc.1999.1948Suche in Google Scholar
2. Serhan CN, Savill J. Resolution of inflammation: the beginning programs the end. Nat Immunol 2005;6:1191–7.10.1038/ni1276Suche in Google Scholar
3. Woodwork KA, Dyke KV. Anti-inflammatory and antirheumatic drugs. In: Craig CR, Stitzel RE, editors. Modern pharmacology with clinical applications, 6th ed. Boston: Little and Brown, 2004:424.Suche in Google Scholar
4. Kumara NK. Identification of strategies to improve research on medicinal plants used in Sri Lanka. In: WHO Symposium. University of Ruhuna, Galle, Sri Lanka, 2001.Suche in Google Scholar
5. Calixto JB, Beirith A, Ferreira J, Santos AR, Cechinel Filho V, Yunes RA. Naturally occurring antinociceptive substances from plants. Phytother Res 2000;14:401–18.10.1002/1099-1573(200009)14:6<401::AID-PTR762>3.0.CO;2-HSuche in Google Scholar
6. Odetola AA, Akojenu SM. Antidiarrhoeal and gastrointestinal potentials of the aqueous extracts of Phyllanthus amarus (Euphorbiaceae). Afr J Med Sci 2000;29:119–22.Suche in Google Scholar
7. Adeneye AA, Benebo AS, Agbaje EO. Protective effect of the aqueous leaf and seed extract of Phyllanthus amarus on alcohol-induced hepatotoxicity in rats. West Afr J Pharmacol Drug Res 2006;22–23:42–50.10.4314/wajpdr.v22i1.14767Suche in Google Scholar
8. Adedapo AA, Adegbayibi AY, Emikpe BO. Some clinico-pathological changes associated with the aqueous extract of the leaves of Phyllanthus amarus in rats. Phytother Res 2005;19:971–6.10.1002/ptr.1768Suche in Google Scholar
9. Appiah-Opong R, Nyarko AK, Dodoo D, Gyang FN, Koram KA, Ayisi NK. Antiplasmodial activity of extracts of Tridax procumbens and Phyllanthus amarus in in vitro Plasmodium falciparum culture systems. Ghana Med J 2011;45:143–50.Suche in Google Scholar
10. Krishnamurti GV, Sheshadri TR. Bitter principle of Phyllanthus niruri L. Proc Indian Acad Sci 1946;357:2712.Suche in Google Scholar
11. Nara IK, Cleve J. Lavergne de, C, Stanislaus, E. Flavonoides de Phyllanthus niruri L., P. urinaria L. et P. nbicculatus L. Rich Plant Med Phytother 1977;11:82–6.Suche in Google Scholar
12. Shale TL, Stirk WA, Van Staden J. Screening of medicinal plants used in Lesotho for antibacterial and anti-inflammatory activity. J Ethnopharmacol 1999;67:347–54.10.1016/S0378-8741(99)00035-5Suche in Google Scholar
13. Evans WC. Pharmacognosy. London: W.B. Saunders, 2002.Suche in Google Scholar
14. Moody JO, Robert VA, Connolly JD, Houghton PJ. Anti-inflammatory activities of the methanol extracts and an isolated furanoditerpene constituent of Sphenocentrum jollyanum Pierre (Menispermaceae). J Ethnopharmacol 2006;104:87–91.10.1016/j.jep.2005.08.051Suche in Google Scholar
15. Sawadogo WR, Boly R, Lompo M, Some N. Anti-inflammatory, analgesic and antipyretic activities of Dicliptera verticillata. Int J Pharmacol 2006;2:435–8.10.3923/ijp.2006.435.438Suche in Google Scholar
16. Hilaly JE, Israili ZH, Lyoussi B. Acute and chronic toxicological studies of Ajuga iva in experimental animals. J Ethnopharmacol 2004;91:43–50.10.1016/j.jep.2003.11.009Suche in Google Scholar
17. Gupta M, Mazunder UK, Sambath Kumbar R, Gomath P, Rajeshwar Y, Kakoti BB, et al. Anti-inflammatory, analgesic and antipyretic effects of methanol extract from Bauhina racemosa stem bark in animal models. J Ethnopharmacol 2005;98: 267–73.10.1016/j.jep.2005.01.018Suche in Google Scholar
18. Perianayagam JB, Sharma SK, Pillai KK. Anti-inflammatory activity of Trichodesma indicum root extract in experimental animals. J Ethnopharmacol 2006;104:410–14.10.1016/j.jep.2005.08.077Suche in Google Scholar
19. Correa CR, Calixto JB. Evidence of participation of B1 and B2 kinin receptors in formalin-induced nociceptive response in mouse. Br J Pharmacol 1993;110:193–8.10.1111/j.1476-5381.1993.tb13791.xSuche in Google Scholar
20. Adedapo AA, Koduru S, Jimoh FO, Masika PJ, Afolayan AJ. Evaluation of the medicinal potentials of the methanol extracts of the leaves and stem of Halleria lucida. Bioresour Technol 2008;99:4158–63.10.1016/j.biortech.2007.08.066Suche in Google Scholar
21. Adedapo AA, Jimoh FO, Afolayan AJ, Masika PJ. Antioxidant activities and phenolic contents of the methanol extracts of the stems of Acokanthera oppositifolia and Adenia gummifera. BMC Complementary Altern Med 2008;8:54.10.1186/1472-6882-8-54Suche in Google Scholar
22. Ayoola GA, Coker HA, Adesegun SA, Adepoju-Bello AA, Obaweya K, Ezennia EC, et al. Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in Southwestern Nigeria. Trop J Pharm Res 2008;7:1019–24.Suche in Google Scholar
23. Lai FR, Wen QB, Li L, Wu H, Li XF. Antioxidant activities of water-soluble polysaccharide extracted from mung bean (Vigna radiata L.) hull with ultrasonic assisted treatment. Carbohydr Polym 2010;81:323–9.10.1016/j.carbpol.2010.02.011Suche in Google Scholar
24. Rajanarayana K, Reddy MS, Chaluvadi MR, Krishna DR. Bioflavonoids classification, pharmacological, biochemical effects and therapeutic potential. Indian J Pharmacol 2001;33:2–16.Suche in Google Scholar
25. Chakraborty A, Devi RK, Rita S, Sharatchandra K, Singh TI. Preliminary studies on anti-inflammatory and analgesic activities of Spilanthes acmella in experimental animal models. Indian J Pharmacol 2004;36:148–50.Suche in Google Scholar
26. Ozaki Y. Anti-inflammatory effects of Curcuma xanthorrhiza Roxb, and its active principle. Chem Pharm Bull 1990;38:1045–8.10.1248/cpb.38.1045Suche in Google Scholar
27. Mossai JS, Rafatullah S, Galal AM, Al-Yahya MA. Pharmacological studies of Rhus retinorrhea. Int J Pharmacol 1995;33:242–6.Suche in Google Scholar
28. Silva GN, Martins FR, Matheus ME. Investigation of anti-inflammatory and antinociceptive activities of Lantana trifolia. J Ethnopharmacol 2005;100:254–9.10.1016/j.jep.2005.02.040Suche in Google Scholar
29. Guay J, Bateman K, Gordon R, Mancini J, Riendeau D. Carrageenan-induced paw edema in rat elicits a predominant prostaglandin E2 (PGE2) response in the central nervous system associated with the induction of microsomal PGE2 synthase-1. J Biol Chem 2004;279:24866–72.10.1074/jbc.M403106200Suche in Google Scholar
30. Antonio MA, Souza Brito AR. Oral anti-inflammatory and anti ulcerogenic activities of a hydroalcoholic extract and partitioned fractions of Turnea ulmifolia (Turneaceae). J Ethnopharmacol 1998;61:215–28.10.1016/S0378-8741(98)00049-XSuche in Google Scholar
31. Cuman RK, Bersani-Amadio CA, Fortes ZB. Influence of type 2 diabetes on the inflammatory response in rat. Inflammation Res 2001;50:460–5.10.1007/PL00000271Suche in Google Scholar PubMed
32. Linardi A, Costa SK, DeSilva GR, Antunes E. Involvement of kinins, mast cells, and sensory neurons in the plasma exudation and paw edema induced by staphylococcal enterotoxin B in the mouse. Eur J Pharmacol 2002;399:235–42.10.1016/S0014-2999(00)00375-7Suche in Google Scholar
33. Vasudevan M, Gunman KK, Parle M. Antinociceptive and anti-inflammatory effects of Thespesia populnea bark extract. J Ethnopharmacol 2007;109:264–70.10.1016/j.jep.2006.07.025Suche in Google Scholar
34. Le Bars D, Gozariu M, Cadden S. Animal models of nociception. Pharmacol Rev 2001;53:597–652.Suche in Google Scholar
35. Amico-Roxas M, Caruso A, Trombadore S, Scifo R, Scapagnini U. Gangliosides antinociceptive effects in rodents. Arch Int Pharmacodyn Ther 1984;272:103–17.Suche in Google Scholar
36. Dubuisson D, Dennis SG. The formalin test: a quantitative study of the analgesic effects of morphine, meperidine and brain stem stimulation in rats and cats. Pain 1977;4:161–74.10.1016/0304-3959(77)90130-0Suche in Google Scholar
37. Tjolsen A, Berge DG, Hunskaar S, Rosland JH, Hole K. The formalin test: an evaluation of the method. Pain 1992;51:5–17.10.1016/0304-3959(92)90003-TSuche in Google Scholar
38. Hunskaar S, Fasmer OB, Hole K. Formalin test in mice: a useful technique for evaluating wild analgesics. J Neurosci Methods 1985;4:69–76.10.1016/0165-0270(85)90116-5Suche in Google Scholar
39. Ghannadi A, Hajhashemi V, Jafarabadi H. An investigation of the analgesic and anti-inflammatory effects of Nigella sativa seed polyphenols. J Med Food 2005;8:488–93.10.1089/jmf.2005.8.488Suche in Google Scholar PubMed
©2015 by De Gruyter
Artikel in diesem Heft
- Frontmatter
- Review
- Cell cycle checkpoints and pathogenesis of HIV-1 infection: a brief overview
- Behavior/Neuroprotection
- Neuroprotective effect of N-acetyl cysteine against streptozotocin-induced memory dysfunction and oxidative damage in rats
- Effect of (4a) a novel 5-HT3 receptor antagonist on chronic unpredictable mild stress induced depressive-like behavior in mice: an approach using behavioral tests battery
- Involvement of opioidergic and serotonergic systems in the analgesic activity of Cissus quadrangularis L. stem extract in mice
- Multiparameter rodent chronic model for complex evaluation of alcoholism-mediated metabolic violations
- Reproduction
- The methanol-soluble fraction of Millettia macrophylla (Fabaceae) stem bark endowed with estrogenic properties has adverse effects on the male reproductive system of Wistar rats
- Cardiovascular Function
- Kolaviron, a biflavonoid fraction from Garcinia kola, protects against isoproterenol-induced injury by mitigating cardiac dysfunction and oxidative stress in rats
- A comparative study to evaluate the cardiovascular risk of selective and nonselective cyclooxygenase inhibitors (COX-Is) in arthritic patients
- Metabolism
- Increased levels of serum γ-glutamyltransferase and uric acid on metabolic, hepatic and kidney parameters in subjects at high altitudes
- Phytotherapy
- Anti-inflammatory and antinociceptive activities of the aqueous leaf extract of Phyllanthus amarus Schum (Euphorbiaceae) in some laboratory animals
- Gastroprotective activity of essential oils from turmeric and ginger
- Decreased metabolism of 13C-caffeine via hepatic CYP1A2 in marasmus and kwashiorkor based on breath test
Artikel in diesem Heft
- Frontmatter
- Review
- Cell cycle checkpoints and pathogenesis of HIV-1 infection: a brief overview
- Behavior/Neuroprotection
- Neuroprotective effect of N-acetyl cysteine against streptozotocin-induced memory dysfunction and oxidative damage in rats
- Effect of (4a) a novel 5-HT3 receptor antagonist on chronic unpredictable mild stress induced depressive-like behavior in mice: an approach using behavioral tests battery
- Involvement of opioidergic and serotonergic systems in the analgesic activity of Cissus quadrangularis L. stem extract in mice
- Multiparameter rodent chronic model for complex evaluation of alcoholism-mediated metabolic violations
- Reproduction
- The methanol-soluble fraction of Millettia macrophylla (Fabaceae) stem bark endowed with estrogenic properties has adverse effects on the male reproductive system of Wistar rats
- Cardiovascular Function
- Kolaviron, a biflavonoid fraction from Garcinia kola, protects against isoproterenol-induced injury by mitigating cardiac dysfunction and oxidative stress in rats
- A comparative study to evaluate the cardiovascular risk of selective and nonselective cyclooxygenase inhibitors (COX-Is) in arthritic patients
- Metabolism
- Increased levels of serum γ-glutamyltransferase and uric acid on metabolic, hepatic and kidney parameters in subjects at high altitudes
- Phytotherapy
- Anti-inflammatory and antinociceptive activities of the aqueous leaf extract of Phyllanthus amarus Schum (Euphorbiaceae) in some laboratory animals
- Gastroprotective activity of essential oils from turmeric and ginger
- Decreased metabolism of 13C-caffeine via hepatic CYP1A2 in marasmus and kwashiorkor based on breath test
