Expression and role of the genes involved in the transport of bile acids in the liver and kidneys in mice
-
Eugène S. Attakpa
,
Naguibou M. Djibril
Abstract
Background: Bile acids are synthesized in the liver from cholesterol.
Methods: This study investigated the impact and expression of different carriers of bile acid in the liver and kidneys. Eight-week-old male mice were used, which were fed for 15 days and divided into two groups: 15 mice fed with standard diet (control group) and another 15 mice fed with a rich diet of 5% cholesterol (second group). Bile acid dosage was based on their oxidation by 7α hydroxyl-steroid dehydrogenize. The mRNA expression was quantitatively analyzed by the real time of polymerase chain reaction (RT-PCR), and the expression of the renal carrier bile acid protein was analyzed by Western blot.
Results and conclusions: The expression of bile salt export pump involved in the uptake of bile acids in the basolateral membrane of hepatocytes revealed no differences between the two groups of mice. However, the expression of multidrug resistance-associated protein 2 was reduced in mice of the second group. Moreover, the expressions of organic anion transporting polypeptide 4, organic anion transporting polypeptide 1, and sodium taurocholate co-transporting polypeptide (Ntcp) involved in the uptake of bile acids in the apical pole of hepatocytes are suppressed in mice of the second group. The expression of multidrug resistance-associated protein 3 involved in the secretion of bile acids in the apical membrane of hepatocytes revealed no significant differences between the two groups. In mice of the second group, blood concentration of bile acids on the last day was increased. In those mice, the expression of intestinal bile acid transporter was reduced in the kidneys compared with the control mice.
©2013 by Walter de Gruyter Berlin Boston
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Articles in the same Issue
- Masthead
- Masthead
- Review
- Cytokines and their role in hyperthermia and heat stroke
- Original Articles
- Expression and role of the genes involved in the transport of bile acids in the liver and kidneys in mice
- Methemoglobin incites primaquine toxicity through single-electron oxidation and modification
- Brain and liver oxidative stress after sertraline and haloperidol treatment in mice
- Frozen embryo transfer: the present practice and beyond
- BRAF gene polymorphism (rs10487888) assessment in chronic periodontitis and peri-implantitis in an Iranian population
- What is the effect of ghrelin on rat uterine contractility in vitro?
- The effects of thiol modulators on nitrergic nerve- and S-nitrosothiols-induced relaxation in duodenum
- Effects of aqueous extract of Musa paradisiaca root on testicular function parameters of male rats
- Short Communication
- Bilateral temporal lobe agenesis with bilateral arachnoid cysts – a rare congenital anomaly
