An in vitro study of the ameliorative role of α-tocopherol on methotrexate-induced oxidative stress in rat heart mitochondria
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Kanchanlata Singh
Abstract
Background: Methotrexate (MTX) is a folic acid antagonist widely used as a cytotoxic chemotherapeutic agent for many malignancies. Its use is limited due to wide-ranging toxicities. MTX generates reactive oxygen species. Here, we investigated the efficacy of vitamin E supplementation on MTX-induced alterations in vitro.
Methods: Rat heart mitochondria were isolated and used to assess the extent of swelling, lipid peroxidation and alterations in mitochondrial-specific enzyme activities caused by the addition of 80 μM MTX in the presence and absence of 1.2 μM vitamin E. Control for both groups was maintained.
Result: MTX substantially affects mitochondrial function by increasing lipid peroxidation and mitochondrial swelling. Significant losses in the activities of the tricarboxylic acid cycle enzymes, complex I, II and IV, and an increase in the activity of calcium ATPase were observed in MTX-treated rat mitochondria. Enrichment of the media with vitamin E led to a reduction in swelling and restoration of enzyme activities.
Conclusions: The present study suggests that vitamin E plays a vital role in suppressing MTX-induced mitochondrial toxicity, and affords protection either by reversing the decline of antioxidants or by direct scavenging of free radicals.
©2012 by Walter de Gruyter Berlin Boston
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Articles in the same Issue
- Masthead
- Masthead
- Original Articles
- Yoyo Bitters, a polyherbal formulation influenced some biochemical parameters in Wistar rats
- Combinatorial cytotoxic effects of Curcuma longa and Zingiber officinale on the PC-3M prostate cancer cell line
- The influence of oxonate-induced hyperuricemia and allopurinol on behavioral reactions of random-bred mice
- Acetylcholinesterase (AChE) inhibitory activity, antioxidant properties and phenolic composition of two Aframomum species
- An in vitro study of the ameliorative role of α-tocopherol on methotrexate-induced oxidative stress in rat heart mitochondria
- Study on blood glucose concentration in patients with diabetes undergoing dental extraction under local anesthesia with and without adrenaline
- Drug use evaluation of diabetes mellitus in hospitalized patients of a tertiary care referral hospital
- Biochemical and morphological changes in Trypanosoma brucei brucei- infected rats treated with homidium chloride and diminazene aceturate
