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Effect of maceration, ultrasound, and microwave-assisted method of extraction on antioxidant activity and phenolic profile of free, esterified, and bound phenolics of Tulaipanji rice

  • Subhajit Mandal , Md. Latifur Rahman , Priya Das , Gouhar Jahan Ashraf , Tarun Kumar Dua , Paramita Paul , Gouranga Nandi and Ranabir Sahu ORCID logo EMAIL logo
Published/Copyright: November 16, 2023

Abstract

The present work was designed to investigate the effect of maceration, ultra-sound, and microwave-assisted extraction on free, esterified, and bound phenolics of Tulaipanji rice (Oryza sativa L.) flour. Antioxidant activity was measured by DPPH, ABTS, SOD, and reducing power assays. HPTLC analysis was performed for identification and quantification of phenolics in different extracts. Total phenolic (TPC) and total flavonoid contents (TFC) were found significantly high (p < 0.05) in microwave-assisted extraction of bound phenolics compared with other methods. Microwave-assisted extracts showed highest DPPH radicals, ABTS radicals, SOD, and reducing power activity. Further, Pearson’s correlation study exhibited a significant positive correlation within % yield, TPC, and TFC. Significant negative correlation was found within % yield, TPC, TFC, and antioxidant activity. The study found that extractive value, antioxidant activity, and phenolic contents varied with the extraction methods. Microwave-assisted extraction technique showed the best performance for extraction as compared to other methods.


Corresponding author: Ranabir Sahu, Department of Pharmaceutical Technology, University of North Bengal, Darjeeling 734013, West Bengal, E-mail:

Award Identifier / Grant number: SRG/2019/001910

Award Identifier / Grant number: 2323/R-2022

Award Identifier / Grant number: No. F. 30-515/2020(BSR);12/02/2020

Acknowledgment

We are grateful to the University of North Bengal for providing facilities for research.

  1. Research ethics: Not applicable.

  2. Author contributions: The authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: The authors state no conflict of interest.

  4. Research funding: The financial support of SERB-SRG (file no. SRG/2019/001910), India, UGC-SRG [No. F. 30-515/2020(BSR);12/02/2020], India and the University assistance/support for the research project (Ref. No. 2323/R-2022), University of North Bengal, India are greatly acknowledged.

  5. Data availability: Not applicable.

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Supplementary Material

This article contains supplementary material (https://doi.org/10.1515/ijfe-2023-0177).


Received: 2023-07-18
Accepted: 2023-10-31
Published Online: 2023-11-16

© 2023 Walter de Gruyter GmbH, Berlin/Boston

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