Low-molecular-weight peptides with potential cardiovascular regulatory functions from Atlantic salmon skin

Wen-Ying Liu; Takuya Miyakawa; Jun Lu; Rui-Zeng Gu; Yun Hua Hsieh; Yumiko Miyauchi; Kana Katsuno; Mu-Yi Cai; Masaru Tanokura

doi:10.1515/ijfe-2020-0072

Article

Low-molecular-weight peptides with potential cardiovascular regulatory functions from Atlantic salmon skin

Wen-Ying Liu , Takuya Miyakawa , Jun Lu , Rui-Zeng Gu , Yun Hua Hsieh , Yumiko Miyauchi , Kana Katsuno , Mu-Yi Cai and Masaru Tanokura

Published/Copyright: August 21, 2020

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From the journal International Journal of Food Engineering Volume 16 Issue 11

Abstract

Salmon skin collagen peptides (SSCPs) have potential for improving physiological conditions such as early alcoholic liver injury, type 2 diabetes and hypertension. Here, we focused on the in vitro effects of SSCPs on vascular function. For the production of SSCPs, alcalase and papain were used to hydrolyse the skin of Atlantic salmon (Salmo salar L.), and their separation was made by reverse-phase high performance liquid chromatography. There were 10 low-molecular-weight peptides newly identified by mass spectrometry. In addition to five peptides previously identified, a total of 15 peptides were applied to an in vitro analysis of cholesterol-reducing, vasorelaxant and antithrombotic activities. The results showed that the SSCPs contained six cholesterol-lowering peptides (Ala-Pro, Leu-Gln, Asn-Val-Gly, Arg-Glu-Arg, Pro-His and Gly-Pro-Arg), two vasorelaxant peptides (Leu-Gln and Pro-His), and four antithrombotic peptides (Gly-Pro-Arg, Arg-Glu-Arg, Val-Asp-Gly-Lys and Val-Arg) as novel candidate peptides with beneficial effects on vascular function. These active peptides were also quantified. This study reveals that several peptides from salmon skin possess bifunctional properties.

Keywords: antithrombotic activity; cholesterol-reducing activity; peptides; salmon; vasorelaxant activity

Corresponding authors: Mu-Yi Cai, Beijing Engineering Research Center of Protein and Functional Peptides, China National Research Institute of Food and Fermentation Industries Co. Ltd., Beijing, 100015, People’s Republic of China. Tel.: +86 10 5321 8267, E-mail: caimuyi@vip.sina.com; and M. Tanokura, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan. Tel.: +81 3 5841 5165, E-mail: amtanok@mail.ecc.u-tokyo.ac.jp

Funding source: National Key Research and Development Program

Award Identifier / Grant number: 2016YFD0400604

Funding source: Japan Society for the Promotion of Science

Award Identifier / Grant number: 23228003

Acknowledgments

We are grateful to CF Haishi Biotechnology Co., Ltd. for providing materials. We thank the staffs of the National Center of Biomedical Analysis for technical assistance in LC/MS/MS.

Author contribution: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: Financial support from the National Key Research and Development Program of China (No. 2016YFD0400604 to MC) is fully acknowledged. This research got support from the Grant-in-Aid for Scientific Research (S) (Grant number, 23228003) of a Japan Society for the Promotion of Science (JSPS).
Conflict of interest statement: The authors declare no conflicts of interest regarding this article.

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Received: 2020-04-06

Accepted: 2020-07-17

Published Online: 2020-08-21

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https://doi.org/10.1515/ijfe-2020-0072

Keywords for this article

antithrombotic activity; cholesterol-reducing activity; peptides; salmon; vasorelaxant activity