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Comparison of Different Physical Technique-Assisted Alkali Methods for the Extraction of Rice Bran Protein and its Characterizations

  • Li-Hui Sun EMAIL logo , Shi-Wen Lv and Lei-Yu He
Published/Copyright: July 22, 2017

Abstract

Ultrasonic, homogenization and microwave were used to assist alkali extraction of rice bran protein, respectively, and the characterizations of rice bran protein were also evaluated. The results of this study showed that the highest extraction yield of protein reached 57.89 % by using ultrasonic-assisted alkali method (U-AM), while only it was 43.74 % by microwave-assisted alkali method (M-AM). Both U-AM and homogenization-assisted alkali methods (H-AM) could effectively improve some properties of proteins such as oil absorption capacity, emulsion stability and foaming capacity, and the effects of ultrasonic were better than those of homogenization. However, protein solubility, water absorption capacity, emulsifying activity and foaming stability were subject to different degrees of impairments by using various physical technique-assisted alkali methods (AMs). Moreover, physical processing also has exhibited appreciable influence on sulfhydryl and disulfide bond contents. Taking all these factors into consideration, ultrasonic-assisted alkali method was a potential method for the extraction of rice bran protein.

Funding statement: This work was supported by Fundamental Research Funds for the Central Universities (Grant/Award Number: ‘DUT16QY31’).

References

[1] Min B, McClung AM, Chen M. Phytochemicals and antioxidant capacities in rice brans of different color. J Food Sci. 2011;76:117–126.10.1111/j.1750-3841.2010.01929.xSearch in Google Scholar PubMed

[2] Luisa Justo M, Claro C, Zeyda M, Stulnig TM, Dolores Herrera M, Rodriguez-Rodriguez R. Rice bran prevents high-fat diet-induced inflammation and macrophage content in adipose tissue. Eur J Nutr. 2016;55:2011–2019.10.1007/s00394-015-1015-xSearch in Google Scholar PubMed

[3] Yılmaz N, Tuncel NB, Kocabıyık H. Infrared stabilization of rice bran and its effects on γ-oryzanol content, tocopherols and fatty acid composition. J Sci Food Agr. 2014;94:1568–1576.10.1002/jsfa.6459Search in Google Scholar PubMed

[4] Rafe A, Vahedi E, Hasan-Sarei AG. Rheology and microstructure of binary mixed gel of rice bran protein-whey: Effect of heating rate and whey addition. J Sci Food Agr. 2016;96:3890–3896.10.1002/jsfa.7586Search in Google Scholar PubMed

[5] Gul K, Yousuf B, Singh AK, Singh P, Wani AA. Rice bran: Nutritional values and its emerging potential for development of functional food-A review. Bioactive Carbohydrates Dietary Fibr. 2015;6:24–30.10.1016/j.bcdf.2015.06.002Search in Google Scholar

[6] Fabian C, Ju Y. A review on rice bran protein: Its properties and extraction methods. Crit Rev Food Sci. 2011;51:816–827.10.1080/10408398.2010.482678Search in Google Scholar PubMed

[7] Juliano BO. Comparative nutritive value of various staple foods. Food Rev Int. 1999;15:399–434.10.1080/87559129909541197Search in Google Scholar

[8] Rafe A, Mousavi SS, Shahidi S. Dynamic rheological behavior of rice bran protein (RBP): Effects of concentration and temperature. J Cereal Sci. 2014;60:514–519.10.1016/j.jcs.2014.09.002Search in Google Scholar

[9] Yutaka S, Takashi M, Mamoru I, Tomohiro M, Sumihiro H, Satoko I, et al. A fibronectin-binding protein from rice bran with cell adhesion activity for animal tumor cells. Biosci Biotech Bioch. 2001;65:1181–1186.10.1271/bbb.65.1181Search in Google Scholar PubMed

[10] Hamada JS. Characterization of protein fractions of rice bran to devise effective methods of protein solubilization. Cereal Chem. 1997;74:662–668.10.1094/CCHEM.1997.74.5.662Search in Google Scholar

[11] Apinunjarupong S, Lapnirun S, Theerakulkait C. Preparation and some functional properties of rice bran protein concentrate at different degree of hydrolysis using bromelain and alkaline extraction. Prep Biochem Biotech. 2009;39:183–193.10.1080/10826060902800858Search in Google Scholar

[12] Thamnarathip P, Jangchud K, Jangchud A, Nitisinprasert S, Tadakittisarn S, Vardhanabhuti B. Extraction and characterisation of riceberry bran protein hydrolysate using enzymatic hydrolysis. Int J Food Sci Tech. 2016;51:194–202.10.1111/ijfs.13008Search in Google Scholar

[13] Tang SH, Hettiarachchy NS, Shellhammer TH. Protein extraction from heat-stabilized defatted rice bran. 1. Physical processing and enzyme treatments. J Agr Food Chem. 2002;50:7444–7448.10.1021/jf025771wSearch in Google Scholar

[14] Chittapalo T, Noomhorm A. Ultrasonic assisted alkali extraction of protein from defatted rice bran and properties of the protein concentrates. Int J Food Sci Tech. 2009;44:1843–1849.10.1111/j.1365-2621.2009.02009.xSearch in Google Scholar

[15] Anderson AK, Guraya HS. Extractability of protein in physically processed rice bran. J Am Oil Chem Soc. 2001;78:969–972.10.1007/s11746-001-0373-1Search in Google Scholar

[16] Phongthai S, Lim S, Rawdkuen S. Optimization of microwave-assisted extraction of rice bran protein and its hydrolysates properties. J Cereal Sci. 2016;70:146–154.10.1016/j.jcs.2016.06.001Search in Google Scholar

[17] Paraman I, Hettiarachchy NS, Schaefer C. Preparation of rice endosperm protein isolate by alkali extraction. Cereal Chem. 2008;85:76–81.10.1094/CCHEM-85-1-0076Search in Google Scholar

[18] Kinsella JE, Whitehead DM. Proteins in whey: Chemical, physical, and functional properties. Adv Food Nutr Res. 1989;33:343–438.10.1016/S1043-4526(08)60130-8Search in Google Scholar PubMed

[19] Wang M, Hettiarachchy NS, Qi M, Burks W, Siebenmorgen T. Preparation and functional properties of rice bran protein isolate. J Agr Food Chem. 1999;47:411–416.10.1021/jf9806964Search in Google Scholar PubMed

[20] Bandyopadhyay K, Misra G, Ghosh S. Preparation and characterisation of protein hydrolysates from Indian defatted rice bran meal. J Oleo Sci. 2008;57:47–52.10.5650/jos.57.47Search in Google Scholar PubMed

[21] Yasumatsu K, Toda J, Sawada K, Wada T, Misaki M, Moritaka S. Studies on function properties of food-grade soybean products. IV. Whipping and emulsifying properties of soybean products. Agric Biol Chem. 1972;36:719.10.1080/00021369.1972.10860321Search in Google Scholar

[22] Rao A, Shallo HE, Ericson AP, Thomas RL. Characterization of soy protein concentrate produced by membrane ultrafiltration. J Food Sci. 2002;67:1412–1418.10.1111/j.1365-2621.2002.tb10299.xSearch in Google Scholar

[23] Xia N, Wang J, Yang X, Yin S, Qi J, Hu L, et al. Preparation and characterization of protein from heat-stabilized rice bran using hydrothermal cooking combined with amylase pretreatment. J Food Eng. 2012;110:95–101.10.1016/j.jfoodeng.2011.12.004Search in Google Scholar

[24] Laemmli UK. Cleavage of structural proteins during assembly of head of bacteriophage-T4. Nature. 1970;227:680–685.10.1038/227680a0Search in Google Scholar PubMed

[25] Beveridge T, Toma SJ, Nakai S. Determination os SH-groups and SS-groups in some food proteins using Ellman’s reagent. J Food Sci. 1974;39:49–51.10.1111/j.1365-2621.1974.tb00984.xSearch in Google Scholar

[26] Wang C, Xu F, Li D, Zhang M. Physico-chemical and structural properties of four rice bran protein fractions based on the multiple solvent extraction method. Czech J Food Sci. 2015;33:283–291.10.17221/462/2014-CJFSSearch in Google Scholar

[27] Segat A, Misra NN, Fabbro A, Buchini F, Lippe G, Cullen PJ, et al. Effects of ozone processing on chemical, structural and functional properties of whey protein isolate. Food Res Int. 2014;66:365–372.10.1016/j.foodres.2014.10.002Search in Google Scholar

[28] Jitngarmkusol S, Hongsuwankul J, Tananuwong K. Chemical compositions, functional properties, and microstructure of defatted macadamia flours. Food Chem. 2008;110:23–30.10.1016/j.foodchem.2008.01.050Search in Google Scholar PubMed

[29] Hendrickx ME, Van Der Plancken I, Van Loey A. Effect of heat-treatment on the physico-chemical properties of egg white proteins: A kinetic study. J Food Eng. 2006;75:316–326.10.1016/j.jfoodeng.2005.04.019Search in Google Scholar

[30] Guan X, Yao H, Chen Z, Shan L, Zhang M. Some functional properties of oat bran protein concentrate modified by trypsin. Food Chem. 2007;101:163–170.10.1016/j.foodchem.2006.01.011Search in Google Scholar

[31] Aletor O, Oshodi AA, Ipinmoroti K. Chemical composition of common leafy vegetables and functional properties of their leaf protein concentrates. Food Chem. 2002;78:63–68.10.1016/S0308-8146(01)00376-4Search in Google Scholar

[32] Wang T, Wang L, Wang R, Chen Z. Effects of freeze-milling on the physicochemical properties of rice protein isolates. LWT-Food Sci Technol. 2016;65:832–839.10.1016/j.lwt.2015.09.016Search in Google Scholar

[33] Ragab DM, Babiker EE, Eltinay AH. Fractionation, solubility and functional properties of cowpea (Vigna unguiculata) proteins as affected by pH and/or salt concentration. Food Chem. 2004;84:207–212.10.1016/S0308-8146(03)00203-6Search in Google Scholar

[34] Zhao Q, Xiong H, Selomulya C, Chen X, Huang S, Ruan X, et al. Effects of spray drying and freeze drying on the properties of protein isolate from rice dreg protein. Food Bioprocess Tech. 2013;6:1759–1769.10.1007/s11947-012-0844-3Search in Google Scholar

[35] Shimada K, Cheftel J. Determination of sulfhydryl groups and disulfide bonds in Heat-Induced gels of soy protein isolate. J Agr Food Chem. 1988;36:147–153.10.1021/jf00079a038Search in Google Scholar

Published Online: 2017-7-22

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