Optimization of Enzyme-Assisted Extraction of Carotenoids Antioxidants from Cordyceps militaris Using Response Surface Methodology
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Bai-Juan Wang
Bai-Juan Wang was born in 1979. She is an Associate Professor at Yunnan Agricultural University, China. She acquired her M.Sc from Yunnan University. She specializes in Biophysics and Yunnan Pu-erh Tea.
Qing-Song Yang was born in 1980. He is an Associate Professor at Yunnan Minzu University, China. He acquired his Ph.D from Kunming Institute of Botany, Chinese Academy of Sciences. He specializes in Medicinal Plant Resources.
,
Ting Chen
Ting Chen was born in 1990. She is a graduate student of Yunnan Agricultural University, China. She specializes in Agricultural electrification and automation and Yunnan Pu-erh Tea.
Xiang-Dong Qin was born in 1975. She is a Professor at Yunnan Agricultural University, China. She acquired his Ph.D from Kunming Institute of Botany, Chinese Academy of Sciences. She specializes in Natural Products Chemistry.
Jun-Rong Ma was born in 1976. She is a lecturer at Yunnan Agricultural University, China. She acquired her M.Sc from Yunnan University. She specializes in Food Chemistry.
Yan Zhao was born in 1982. She is a senior experimentalist at Yunnan Agricultural University, China. She acquired her Ph.D from Kunming University of Science and Technology. She specializes in Biochemistry.
Abstract
Response surface methodology (RSM) with a Box–Behnken design (BBD) was used to optimize the extraction conditions of carotenoid antioxidants from Cordyceps militaris. An enzyme-assisted extraction (EAE) method was developed and optimized to enhance 2,2-diphenyl-1-picrylhydrazil (DPPH) radical-scavenging activity of carotenoid antioxidants from C. militaris. The optimum conditions were as follows: a pH of 4.1, an extraction time of 40.4 min, an extraction temperature of 48.7 °C and 0.43 % enzyme (cellulose: pectinase, 1:1, w/w) concentration. Under these conditions, the DDPH radical-scavenging activity of C. militaris extracts was 81.62±2.26 %, indicating that the BBD is an efficient approach to develop mathematical models for predictingcarotenoids antioxidant extraction from C. militaris. The successful extraction of carotenoid antioxidants from C. militaris provides a basis for the development and utilization of C. militaris resources.
Funding statement: This work was supported by Applied Basic Research Programs of Science and Technology Commission Foundation of Yunnan Province (No. 2015FB147), the Educational Commission of Yunnan Province, China (2014C070Y), National Natural Science Foundation of China (31460137) and Yunnan Agricultural University Natural Science Foundation for Young Scientists Project (2015ZR15).
About the authors
Bai-Juan Wang was born in 1979. She is an Associate Professor at Yunnan Agricultural University, China. She acquired her M.Sc from Yunnan University. She specializes in Biophysics and Yunnan Pu-erh Tea.
Qing-Song Yang was born in 1980. He is an Associate Professor at Yunnan Minzu University, China. He acquired his Ph.D from Kunming Institute of Botany, Chinese Academy of Sciences. He specializes in Medicinal Plant Resources.
Ting Chen was born in 1990. She is a graduate student of Yunnan Agricultural University, China. She specializes in Agricultural electrification and automation and Yunnan Pu-erh Tea.
Xiang-Dong Qin was born in 1975. She is a Professor at Yunnan Agricultural University, China. She acquired his Ph.D from Kunming Institute of Botany, Chinese Academy of Sciences. She specializes in Natural Products Chemistry.
Jun-Rong Ma was born in 1976. She is a lecturer at Yunnan Agricultural University, China. She acquired her M.Sc from Yunnan University. She specializes in Food Chemistry.
Yan Zhao was born in 1982. She is a senior experimentalist at Yunnan Agricultural University, China. She acquired her Ph.D from Kunming University of Science and Technology. She specializes in Biochemistry.
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Articles in the same Issue
- Articles
- A Method to Analyze the Protein Denaturation of Whole Quail Egg Based on in situ NMR and MRI
- Non-linear Rheological Properties of Soy Protein Isolate Dispersions and Acid-Induced Gels
- Finite Element modeling of Mechanical Loading-Pumpkin Peel and flesh
- Frequency Sweep Test and Modal Analysis of Watermelon during Transportation
- Identification and Classification of Three Iranian Rice Varieties in Mixed Bulks Using Image Processing and MLP Neural Network
- The Impact of Heat-Moisture Treatment on Physicochemical Properties and Retrogradation Behavior of Sweet Potato Starch
- Drying Kinetics and Quality Attributes of Peach Cylinders as Affected by Osmotic Pretreatments and Infrared Radiation Drying
- Hot-Melt Fluidized Bed Encapsulation of Citric Acid with Lipid
- Optimization of Enzyme-Assisted Extraction of Carotenoids Antioxidants from Cordyceps militaris Using Response Surface Methodology
- Optimization of Ultrasonic-Assisted Extraction for Pinocembrin from Flospopuli Using Response Surface Methodology
