Abstract
In this study, 1 wt% lecithin (–), chitosan (+), and λ-carrageenan (–) were prepared to manufacture multiple-layered liposomes with optimal formulations developed in a previous study by using layer-by-layer electrostatic deposition. We observed their particle size, ζ-potential, sedimentation behavior, and microstructure for 6 weeks. Multiple-layered liposomes were quenched with calcein to evaluate stability in terms of factors such as encapsulation efficiency and released amount of calcein. The particle size of multi-layered liposomes increased with storage periods and the ζ-potential of multiple-layered liposomes gained a neutral charge. Interestingly, negatively charged layered liposomes were smaller than positively charged layered liposomes and showed a lower polydispersity index. Moreover, the ζ-potential did not apparently change compared to positively charged layered liposomes. For the calcein release study, multiple-layered liposomes significantly sustained quenched calcein more than that observed using non-layered liposomes. This study showed that it was possible to increase the thickness of the liposome surface and to manipulate its charge using chitosan and λ-carrageenan through electrostatic deposition. Results showed that manufacturing negatively charged multiple-layer (over 4-layer) liposomes with charged biopolymer improved the physicochemical stability of liposomes.
Funding source: National Research Foundation of Korea
Award Identifier / Grant number: NRF-2014R1A1A3053860
Funding statement: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MOE) (No. NRF-2014R1A1A3053860).
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©2016 by De Gruyter
Articles in the same Issue
- Frontmatter
- Research Articles
- Torque Measurement in Real Time during Mixing and Kneading of Bread Dough with High Content of Resistant Maize Starch and Enzymes
- A Novel Color Parametric Technique for Kinetic Evaluation of Thermal Degradation and Stability of Fresh-Cut Yacon Slices
- Modelling and Multi-objective Optimisation of the VHP Pouch Packaging Sterilisation Process
- Ultrasound-Assisted Rehydration of Dried Sea Cucumber (Stichopus japonicus) – Kinetics
- Change of Multiple-Layered Phospholipid Vesicles Produced by Electrostatic Deposition of Polymers during Storage
- Mechanical and Water Barrier Properties of Zein–Corn Starch Composite Films as Affected by Gallic Acid Treatment
- Hot-air Drying Kinetics of Yam Slices under Step Change in Relative Humidity
- Study of Spray-dried Yoghurt Production in a Pilot-scale Equipment Using Drying Agents to Reduce Wall Deposition
Articles in the same Issue
- Frontmatter
- Research Articles
- Torque Measurement in Real Time during Mixing and Kneading of Bread Dough with High Content of Resistant Maize Starch and Enzymes
- A Novel Color Parametric Technique for Kinetic Evaluation of Thermal Degradation and Stability of Fresh-Cut Yacon Slices
- Modelling and Multi-objective Optimisation of the VHP Pouch Packaging Sterilisation Process
- Ultrasound-Assisted Rehydration of Dried Sea Cucumber (Stichopus japonicus) – Kinetics
- Change of Multiple-Layered Phospholipid Vesicles Produced by Electrostatic Deposition of Polymers during Storage
- Mechanical and Water Barrier Properties of Zein–Corn Starch Composite Films as Affected by Gallic Acid Treatment
- Hot-air Drying Kinetics of Yam Slices under Step Change in Relative Humidity
- Study of Spray-dried Yoghurt Production in a Pilot-scale Equipment Using Drying Agents to Reduce Wall Deposition