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Characterization and Antioxidant Activity of Flash-Assisted Extracted Dihydroquercetin from Wood Sawdust of Larix gmelinii Using a Response Surface Methodology

  • Jianxia Li , Qiaohui Zhang , Jie Cui , Yaping Ning , Fengjun Wang , Jie Ouyang and Jianzhong Wang EMAIL logo
Published/Copyright: May 13, 2016

Abstract

We investigate a flash-assisted extraction of dihydroquercetin (DHQ) from wood sawdust of Larix gmelinii by response surface methodology. Box–Behnken design optimized the extraction conditions, and the highest DHQ yield (6.15 %) was obtained under optimal extraction conditions: FAE time (3 min), extraction time (3 h), solid-to-liquid (g/mL) ratio (1:15), and ethanol concentration (75 %). After purification, the DHQ purity was 96 % as determined by HPLC and was further characterized by FTIR. Purified DHQ’s antioxidant activities were confirmed in vitro by determining DPPH and ABTS radical scavenging activities and reducing Fe3+ power. In the DPPH and ABTS radical scavenging activity assays, DHQ displayed prominent antioxidant activities with low IC50 values (11.568 and 12.475 μg/mL, respectively). In reducing Fe3+ power assay, high DHQ absorbance values showed that DHQ had higher reducing power than butylated hydroxyl toluene and had nearly the same consistent power as ascorbic acid at the same doses.

References

1. Wang Y, Zu YG, Long JJ, Fu YJ, Li SM, Zhang DY, et al. Enzymatic water extraction of taxifolin from wood sawdust of Larix gmelinii (Rupr.) Rupr. and evaluation of its antioxidant activity. Food Chem 2011;126:1178–85.10.1016/j.foodchem.2010.11.155Search in Google Scholar

2. Yang L, Sun XW, Yang FJ, Zhao CJ, Zhang L, Zu YG. Application of ionic liquids in the microwave-assisted extraction of proanthocyanidins from Larix gmelinii bark. Int J Mol Sci 2012;13:5163–78.10.3390/ijms13045163Search in Google Scholar

3. Liu ZZ, Ma CH, Yang L, Zu YG. Process optimization of ultrasonic-assisted extraction of arabinogalactan from dihydroquercetin extracted residues by response surface methodology and evaluation of its antioxidant activity. J Chem 2013;1–9.10.1155/2013/346810Search in Google Scholar

4. Lee SB, Cha KH, Selenge D, Solongo A, Nho CW. The chemopreventive effect of taxifolin is exerted through ARE-dependent gene regulation. Biol Pharm Bull 2007;30:1074–9.10.1248/bpb.30.1074Search in Google Scholar

5. Chatzopoulou A, Karioti A, Gousiadou C, Vivancos VL, Kyriazopoulos P, Golegou S, et al. Depsides and other polar constituents from Origanum dictamnus L. and their in vitro antimicrobial activity in clinical strains. J Agric Food Chem 2010;58:6064–8.10.1021/jf904596mSearch in Google Scholar

6. Delporte C, Backhouse N, Erazo S, Negrete R, Vidal P, Silva X, et al. Analgesic-antiinflammatory properties of Proustia pyrifolia. J Ethnopharmacol 2005;99:119–24.10.1016/j.jep.2005.02.012Search in Google Scholar

7. Theriault A, Wang Q, Van Iderstine SC, Chen B, Franke AA, Adeli K. Modulation of hepatic lipoprotein synthesis and secretion by taxifolin, a plant flavonoid. J Lipid Res 2000;41:1969–79.10.1016/S0022-2275(20)32358-0Search in Google Scholar

8. Willfor SM, Ahotupa MO, Hemming JE, Reunanen MH, Eklund PC, Sjoholm RE, et al. Antioxidant activity of knot wood extractives and phenolic compounds of selected tree species. J Agric Food Chem 2003;51:7600–6.10.1021/jf030445hSearch in Google Scholar PubMed

9. Bais HP, Walker TS, Kennan AJ, Stermitz FR, Vivanco JM. Structure dependent phytotoxicity of catechins and other flavonoids: flavonoid conversions by cell-free protein extracts of Centaurea maculosa (spotted knapweed) roots. J Agric Food Chem 2003;51:897–901.10.1021/jf020978aSearch in Google Scholar PubMed

10. Brignolas F, Lacroix B, Lieutier F, Sauvard D, Drouet A, Claudot AC, et al. Induced responses in phenolic metabolismin two Norway spruce clones after wounding and inoculations with Ophiostoma polonicum, a bark beetle-associated fungus. Plant Physiol 1995;109:821–7.10.1104/pp.109.3.821Search in Google Scholar PubMed PubMed Central

11. Yang LJ, Chen W, Ma SX, Gao YT, Huang R, Yan SJ, et al. Host–guest system of taxifolin and native cyclodextrin or its derivative: preparation, characterization, inclusion mode, and solubilization. Carbohydr Polym 2011;85:629–37.10.1016/j.carbpol.2011.03.029Search in Google Scholar

12. Barton GM, Gardner JAF. Determination of dihydroquercetin in Douglas fir and western larch wood. Anal Chem 1958;30:279–81.10.1021/ac60134a033Search in Google Scholar

13. Tyukavkina Nonna A, Naumov Viktor V. Method for Producing Dihydroquercetin [P] 1995, RU2038094.Search in Google Scholar

14. Alekseevieh LS. Method of Dihydroquereetin production [P] 2008, RU2330677.Search in Google Scholar

15. Li M, Ngadi MO, Ma Y. Optimisation of pulsed ultrasonic and microwave-assisted extraction for curcuminoids by response surface methodology and kinetic study. Food Chem 2014;165:29–34.10.1016/j.foodchem.2014.03.115Search in Google Scholar PubMed

16. Huie CW. A review of modern sample-preparation techniques for the extraction and analysis of medicinal plants. Anal Bioanal Chem 2002;373:23–30.10.1007/s00216-002-1265-3Search in Google Scholar PubMed

17. Rohner CA, Matsler AL, Siebenmorgen TJ. Comparison of three extraction systems for determining surface lipid content of thickness fractionated milled rice. Cereal Chem 2004;81:544–8.10.1094/CCHEM.2004.81.4.544Search in Google Scholar

18. Ma CH, Li W, Sun Z, Huang JM, Yang L, Zu YG, et al. Optimizing extraction technology of dihyroquercetin from larch wood by ethanol pretreatment before pulping. China Pulp Paper 2010;29:78–80.Search in Google Scholar

19. Chai J, Fu JH, Zhang WP, Zheng YN, Liu WC. Optimization of cellulase-assisted extraction of dihydroquercetin by response surface methodology. Food Sci 2014;35:93–7.Search in Google Scholar

20. Liu YZ. Principle and practice of smashing tissue extraction and herbal blitzkrieg extractor. Chinese J Nat Med 2007;5:401–7.10.3736/jcim20070501Search in Google Scholar

21. Xie J, Li HJ, Zhu XY, Yang F, Wang P. Optimization of homogenate extraction for arctiin from Arctium lappa L. by response surface methodology. Food Sci 2010;31:33–6.Search in Google Scholar

22. Fan CJ, Jiang DF, Ling ZS. Optimization of flash-type extraction technology of baicalin from Scutellaria baicalensis by response surface methodology. Chinese J Experimental Traditional Med Formulae. 2012;18:48–51.Search in Google Scholar

23. Liu Y, Wang S. Extraction and antioxidaion of dihydroquercetin. Chem Res Appl 2011;23:78–80.10.1002/nadc.19750230414Search in Google Scholar

24. Wang Y, Wang S. Determination of dihydroquercetin in larch by RP-HPLC. Chem Eng 2009;161:22–4.Search in Google Scholar

25. Shalaby EA, Shanab SMM. Comparison of DPPH and ABTS assays for determining antioxidant potential of water and methanol extracts of Spirulina platensis. Indian J Geo-Marine Sci 2013;42:556–64.Search in Google Scholar

26. Li X, Wang Z, Wang L, Walid E, Zhang H. Ultrasonic-assisted extraction of polysaccharides from Hohenbuehelia serotina by response surface methodology. Int J Biol Macromol 2012;51:523–530.10.1016/j.ijbiomac.2012.06.006Search in Google Scholar PubMed

27. Li PQ, Zhou LG, Mou Y, Mao ZL. Extraction optimization of polysaccharide from Zanthoxylum bungeanum using RSM and its antioxidant activity. Int J Biol Macromol 2015;7:19–27.10.1016/j.ijbiomac.2014.07.057Search in Google Scholar

28. Yang YC, Li J, Zu YG, Fu YJ, Luo M, Wu N, et al. Optimisation of microwave-assisted enzymatic extraction of corilagin and geraniin from Geranium sibiricum Linne and evaluation of antioxidant activity. Food Chem 2010;122:373–80.10.1016/j.foodchem.2010.02.061Search in Google Scholar

29. Yin GH, Dang YL. Optimization of extraction technology of the Lycium barbarum polysaccharides by Box–Behnken statistical design. Carbohydr Polym 2008;74:603–10.10.1016/j.carbpol.2008.04.025Search in Google Scholar

30. Yetilmezsoy K, Demirel S, Vanderbei RJ. Response surface modeling of Pb (II) removal from aqueous solution by Pistacia vera L.: Box–Behnken experimental design. J Hazard Mater 2009;171:551–62.10.1016/j.jhazmat.2009.06.035Search in Google Scholar PubMed

31. Karazhiyan H, Razavi S, Phillips GO. Extraction optimization of a hydrocolloid extract from cress seed (Lepidium sativum) using response surface methodology. Food Hydrocolloids 2011;25:915–20.10.1016/j.foodhyd.2010.08.022Search in Google Scholar

32. Zhou Q, Sun SQ, Du DG, Liang XY, Yang XR. Real time monitor of rutin stability during heating by fourier transform infrared spectroscopy. Spectrosc Spect Anal 2000;20:195–8.Search in Google Scholar

33. Kanatt SR, Chander R, Sharma A. Antioxidant potential of mint (Mentha spicata L.) in radiation-processed lamb meat. Food Chem 2007;100:451–8.10.1016/j.foodchem.2005.09.066Search in Google Scholar

34. Habeebullah SF, Nielsen NS, Jacobsen C. Antioxidant activity of potato peel extracts in a fish-rapeseed oil mixture and in oil-in-water emulsions. J Am Oil Chem Soc 2010;87:1319–32.10.1007/s11746-010-1611-0Search in Google Scholar

Published Online: 2016-5-13
Published in Print: 2016-8-1

©2016 by De Gruyter

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