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Potential Use of Tuna (Thunnus albacares) by-product: Production of Antioxidant Peptides and Recovery of Unsaturated Fatty Acids from Tuna Head

  • Dayse Oliveira EMAIL logo , Daniela Bernardi , Fernanda Drummond , Fabiana Dieterich , Wilson Boscolo , Carolina Leivas , Elaine Kiatkoski and Nina Waszczynskyj
Published/Copyright: May 6, 2017

Abstract

Tuna by-products were subjected to enzymatic hydrolysis with Alcalase (enzyme to substrate ratio 1 : 200 w/w; 60 °C; pH 6.5, 120 min) rendering a tuna protein hydrolysate (TPH) with 9.24 % degree of hydrolysis (DH). The antioxidant capacity of TPH determined by the methods of ferric reducing antioxidant power (FRAP) and Trolox equivalent antioxidant capacity (TEAC) were similar and 10 times lower than the result obtained by oxygen radical absorbance capacity (ORAC). The total amino acid profile indicated that 42.15 % are composed of hydrophobic amino acids and 7.7 % of aromatics, with leucine being found in the highest quantity (17.85 %). The fatty acid profile of the oil recovered by centrifugation of the TPH – as determined by a gas chromatograph – was characterized by a high percentage of polyunsaturated fatty acids (PUFAs) (39.06 %), mainly represented by the fatty acids ω3, docosahexaenoic acid (27.15 %) and eicosapentaenoic acid (6.05 %). The simultaneous recovery of unsaturated fatty acids and antioxidant peptides can add value to tuna by-products, assisting in the efficient management of fishing industry waste.

Acknowledgements

The authors would like to thank CAPES (Higher Education Personnel Training Coordination - Brazil), the company Brazil Fishes and the company FALBOM Agroindustrial Ltda. for the support.

The authors declare that there are no conflicts of interest.

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Published Online: 2017-5-6

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