Assessment and Separation of Angiotensin I-Converting Enzyme Inhibitory Peptides in Chinese Soypaste
-
Fengjuan Li
,
Kohji Yamaki
,
Yongqiang Cheng
Abstract
A Chinese soypaste-derived fraction with potent angiotensin I-converting enzyme (ACE) inhibitory activity (IC50 = 25.9 μg/mL) was obtained by treating soypaste extract with 80% ethanol. The result of gradient reversed-phase high-performance liquid chromatography (RP-HPLC) suggested that bioactive peptides bearing some polarity groups made a substantial contribution to the ACE inhibitory activity. By mass spectrometric analysis, a component was separated as Glu-Ser-Gly-Asp which was then found to act in a dose-dependent manner against ACE activity as a non-competitive inhibitor, with an IC50 value of 2.297 mM.
Funding statement: Funding: This work was supported by Kirin Holdings Co., Ltd. (former Kirin Brewery Co., Ltd.), Tokyo, during UNU-Kirin Fellowship Programme at National Food Research Institute (NFRI), Tsukuba, Japan, in 2012–13, and its Follow-Up Project in 2013–15. This work was also within the framework of the fund project (Grant No. 31101329) entitled “Generation mechanism of angiotensin-converting enzyme inhibitors in traditional soypaste” from National Natural Science Foundation of China (NSFC).
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©2015 by De Gruyter
Artikel in diesem Heft
- Frontmatter
- Research Articles
- Modeling and Improving the Performance of Cabinet Solar Dryer Using Computational Fluid Dynamics
- Evaluation of Chemical, Biochemical and Microbiological Quality in Tomato Using Multivariate Analysis
- Effects of the Addition of Grape Seed Powder on the Thermorheological Properties of Frying Batters
- Degradation of Vitamin E in Nanoemulsions during Storage as Affected by Temperature, Light and Darkness
- Effect of Different Drying Processes on the Physicochemical and Antioxidant Properties of Thinned Young Apple
- Mechanical and Sensory Evaluation of Noodles Incorporated with Betel Leaf Extract
- Mathematical Modeling of Thin-Layer Solar Drying of Eland (Taurotragus oryx) Jerky
- Thin-Layer Drying Model of Rambutan (Nephelium lappaceum L.) Kernel and Its Application in Fat Extraction Process
- Color Change Kinetics of Tea Leaves During Microwave Drying
- Influence of Blanching Pretreatment on the Drying Characteristics of Cherry Tomato and Mathematical Modeling
- Studies on Grinding and Extraction of Oil from Fenugreek (Trigonella foenum-graecum) Seed
- Effect of Drum Drying on Physico-chemical Characteristics of Dragon Fruit Peel (Hylocereus polyrhizus)
- Standardization of Curing and Microwave Drying of Turmeric (Curcuma longa) Rhizomes
- Shorter Communication
- Assessment and Separation of Angiotensin I-Converting Enzyme Inhibitory Peptides in Chinese Soypaste
Artikel in diesem Heft
- Frontmatter
- Research Articles
- Modeling and Improving the Performance of Cabinet Solar Dryer Using Computational Fluid Dynamics
- Evaluation of Chemical, Biochemical and Microbiological Quality in Tomato Using Multivariate Analysis
- Effects of the Addition of Grape Seed Powder on the Thermorheological Properties of Frying Batters
- Degradation of Vitamin E in Nanoemulsions during Storage as Affected by Temperature, Light and Darkness
- Effect of Different Drying Processes on the Physicochemical and Antioxidant Properties of Thinned Young Apple
- Mechanical and Sensory Evaluation of Noodles Incorporated with Betel Leaf Extract
- Mathematical Modeling of Thin-Layer Solar Drying of Eland (Taurotragus oryx) Jerky
- Thin-Layer Drying Model of Rambutan (Nephelium lappaceum L.) Kernel and Its Application in Fat Extraction Process
- Color Change Kinetics of Tea Leaves During Microwave Drying
- Influence of Blanching Pretreatment on the Drying Characteristics of Cherry Tomato and Mathematical Modeling
- Studies on Grinding and Extraction of Oil from Fenugreek (Trigonella foenum-graecum) Seed
- Effect of Drum Drying on Physico-chemical Characteristics of Dragon Fruit Peel (Hylocereus polyrhizus)
- Standardization of Curing and Microwave Drying of Turmeric (Curcuma longa) Rhizomes
- Shorter Communication
- Assessment and Separation of Angiotensin I-Converting Enzyme Inhibitory Peptides in Chinese Soypaste
