Abstract
Plantaricin ZJ217 was continually purified by XAD 1180, cation exchange chromatography, gel chromatography, and high performance liquid chromatography (HPLC) system. The peptide functioned as bactericidal, but did not lead to lysis of cells. Considering the potassium efflux experiment, pores may be formed in the surface of cell membrane. Fifteen of twenty amino acids identified by Edman degradation indicated that it may be a novel bacteriocin as no bacteriocin shared similar sequences. This bacteriocin exhibited strong heat stability (121°C, 30 min) and pH stability (pH 2.0–6.0). It was sensitive to proteinase K, trypsin, papain, and pepsin. This bacteriocin inhibited growth of methicillin-resistant Staphylococcus aureus (MRSA) and other bacteria.
Acknowledgments
This project was supported by the National High Technology Research and Development Program (“863” Program) of China (2012AA022105B), the National Natural Science Foundation of China (No. 31071513 and 31271821), the Natural Science Foundation of Zhejiang Province (No. Z3110399), the International Science and Technology Cooperation Program of China (2013DFA32330), and Scientific Research Foundation of Education Department of Zhejiang Province (1110kz0414207).
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Note:
Authors Xuan Zhu and Lei Shen contributed equally to this paper.
©2015 by De Gruyter
Articles in the same Issue
- Frontmatter
- Research Articles
- Investigation of UF and MF Membrane Residual Fouling in Full-Scale Dairy Production Using FT-IR to Quantify Protein and Fat
- Mathematical Modeling of Betanin Extraction from Red Beet (Beta vulgaris L.) by Solid–Liquid Method
- Rapid Discrimination of Apple Varieties via Near-Infrared Reflectance Spectroscopy and Fast Allied Fuzzy C-Means Clustering
- Formulation Development of Multilayered Fish Oil Emulsion by using Electrostatic Deposition of Charged Biopolymers
- Reduction of Turbidity of Beet Sugar Solutions by Mechanical and Chemical Treatment
- Purification of a Bacteriocin from Lactobacillus plantarum ZJ217 Active Against Methicillin-Resistant Staphylococcus aureus
- Influence of Different Wall Materials on the Microencapsulation of Virgin Coconut Oil by Spray Drying
- A CFD Study of the Effects of Feed Diameter on the Pressure Drop in Acyclone Separator
- Electrolyzed Water Generated Using a Circulating Reactor
- Determination of Volatile Compounds of Chinese Traditional Aromatic Sunflower Seeds (Helianthus annulus L.)
- Static Rheological Study of Ocimum basilicum Seed Gum
- Finite Element Model to Predict the Bioconversion Rate of Glucose to Fructose using Escherichia coli K12 for Sugar Production from Date
- Influence of Hot Bed Spray Dryer Parameters on Physical Properties of Peppermint (Mentha piperita L.) Tea Powder
- Foam-Mat Drying of Muskmelon
- Simulating Continuous Time Production Flows in Food Industry by Means of Discrete Event Simulation
- Shorter Communication
- Effect of Vacuum Soaking on the Properties of Soybean (Glycine max (L.) Merr.)
Articles in the same Issue
- Frontmatter
- Research Articles
- Investigation of UF and MF Membrane Residual Fouling in Full-Scale Dairy Production Using FT-IR to Quantify Protein and Fat
- Mathematical Modeling of Betanin Extraction from Red Beet (Beta vulgaris L.) by Solid–Liquid Method
- Rapid Discrimination of Apple Varieties via Near-Infrared Reflectance Spectroscopy and Fast Allied Fuzzy C-Means Clustering
- Formulation Development of Multilayered Fish Oil Emulsion by using Electrostatic Deposition of Charged Biopolymers
- Reduction of Turbidity of Beet Sugar Solutions by Mechanical and Chemical Treatment
- Purification of a Bacteriocin from Lactobacillus plantarum ZJ217 Active Against Methicillin-Resistant Staphylococcus aureus
- Influence of Different Wall Materials on the Microencapsulation of Virgin Coconut Oil by Spray Drying
- A CFD Study of the Effects of Feed Diameter on the Pressure Drop in Acyclone Separator
- Electrolyzed Water Generated Using a Circulating Reactor
- Determination of Volatile Compounds of Chinese Traditional Aromatic Sunflower Seeds (Helianthus annulus L.)
- Static Rheological Study of Ocimum basilicum Seed Gum
- Finite Element Model to Predict the Bioconversion Rate of Glucose to Fructose using Escherichia coli K12 for Sugar Production from Date
- Influence of Hot Bed Spray Dryer Parameters on Physical Properties of Peppermint (Mentha piperita L.) Tea Powder
- Foam-Mat Drying of Muskmelon
- Simulating Continuous Time Production Flows in Food Industry by Means of Discrete Event Simulation
- Shorter Communication
- Effect of Vacuum Soaking on the Properties of Soybean (Glycine max (L.) Merr.)