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Depolymerization of lignin by extracellular activity of Pycnoporus cinnabarinus, to obtain cellulose

  • Raymundo Guzmán Gil , Oscar Manuel González Brambila , Hugo Velasco Bedrán , Julio César García Martínez , José Antonio Colín Luna and Margarita Mercedes González Brambila ORCID logo EMAIL logo
Published/Copyright: October 28, 2022
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International Journal of Chemical Reactor Engineering
From the journal International Journal of Chemical Reactor Engineering Volume 21 Issue 4

Abstract

Cellulose can be used to produce biofuels and many other products like pharmaceutical goods, food supplements, cosmetics, bio-plastics, etc. Lignocellulosic materials, like O. ficus indica residuals, are a heterogeneous biopolymer formed mainly by lignin, hemicellulose and cellulose. Lignin provides protection to the plants against chemical and microbial degradation, but it can be degraded by white rot fungi species, like Pycnoporus cinnabarinus. Since cellulose molecules are arranged in regular bundles enveloped by hemicellulose and lignin molecules, it is necessary to brake lignin and hemicellulose molecules to recover cellulose for its use in bioprocess. In this work, a biotechnological process for cellulose recovery from cactus waste through depolymerization of lignin by P. cinnabarinus, is presented. The delignification is carried out by aerobic culture in batch stirred bioreactors, with a liquid culture medium enriched with nutrients and minerals with O. ficus indica residuals as the unique carbon source, during eight-day span under continuous feeding of oxygen. A factorial design of experiments (DOE) for eight sets of factor values was selected for this study. The factors were: particle size, pH level, and process temperature. For each experiment, biomass, total reducing carbohydrates (TRC) and dissolved oxygen (DO) concentrations were measured every 24 h. At the end of each experiment, the percentage of delignification, and cellulose recovery was measured by Infrared (IR) spectroscopy. Up to 67% of delignification and 22% of cellulose recovery were obtained by the process. These results were analyzed by a factorial DOE in order to maximize each response individually and to optimize both responses together. The delignification of Opuntia ficus indica thorns has not been previously reported to our knowledge.

Keywords: bioprocess optimization; cellulose; delignification; Opuntia ficus indica ; Pycnoporus cinnabarinus
Corresponding author: Margarita Mercedes González Brambila, Universidad Autónoma Metropolitana-Azcapotzalco, Av. San Pablo #180, Col. Reynosa, Tamps, Azcapotzalco, CP 02200, México City, México, E-mail:

Acknowledgements

We want to thank the Consejo Nacional de Ciencia y Tecnología, the Universidad Autónoma Metropolitana – Unidad Azcapotzalco, and the Process Engineering graduate program for its support in the development and conclusion of this research.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Conflict of interest statement: The authors declare no conflicts of interest regarding this article.

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Received: 2022-02-21
Accepted: 2022-09-22
Published Online: 2022-10-28

© 2022 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Frontmatter
  2. Special Issue Articles
  3. Preface of the special issue dedicated to the International Energy Conference, IEC 2021: sustainable energy as a platform for post-pandemic economic recovery
  4. Hydrodesulfurization of 4,6–Dimethyldibenzothiophene on NiMoP/γ–Al2O3 catalyst under reactive distillation conditions in a micro trickle bed reactor: solvent and temperature effect
  5. Bubble Column Bioreactor using native non-genetically modified organisms: a remediation alternative by hydrocarbon-polluted water from the Gulf of Mexico
  6. Depolymerization of lignin by extracellular activity of Pycnoporus cinnabarinus, to obtain cellulose
  7. Robust model-based control of a packed absorption column for the natural gas sweetening process
  8. The degradation of an aromatic organic compound by Aspergillus niger var tubingensis Ed8 produces metabolites that reduce Cr (VI)
  9. Analytical approach in higher predict residual error on MHD mixed convective motion of MoS2 engine-oil based nanofluid
  10. Photocatalytic degradation of naproxen using single-doped TiO2/FTO and co-doped TiO2-VO2/FTO thin films synthesized by sonochemistry
  11. Macroscopic analysis of chemical looping combustion with ilmenite versus conventional oxides as oxygen carriers
  12. Robust control designs for microalgae cultivation in continuous photobioreactors
  13. Evaluation of a rough-surface evaporator applied to an absorption heat transformer for water desalination
  14. Optimized infrared-assisted extraction to obtain total lipid from microalgae Scenedesmus obliquus: a green approach
https://doi.org/10.1515/ijcre-2022-0037
Keywords for this article
bioprocess optimization; cellulose; delignification; Opuntia ficus indica; Pycnoporus cinnabarinus

Articles in the same Issue

  1. Frontmatter
  2. Special Issue Articles
  3. Preface of the special issue dedicated to the International Energy Conference, IEC 2021: sustainable energy as a platform for post-pandemic economic recovery
  4. Hydrodesulfurization of 4,6–Dimethyldibenzothiophene on NiMoP/γ–Al2O3 catalyst under reactive distillation conditions in a micro trickle bed reactor: solvent and temperature effect
  5. Bubble Column Bioreactor using native non-genetically modified organisms: a remediation alternative by hydrocarbon-polluted water from the Gulf of Mexico
  6. Depolymerization of lignin by extracellular activity of Pycnoporus cinnabarinus, to obtain cellulose
  7. Robust model-based control of a packed absorption column for the natural gas sweetening process
  8. The degradation of an aromatic organic compound by Aspergillus niger var tubingensis Ed8 produces metabolites that reduce Cr (VI)
  9. Analytical approach in higher predict residual error on MHD mixed convective motion of MoS2 engine-oil based nanofluid
  10. Photocatalytic degradation of naproxen using single-doped TiO2/FTO and co-doped TiO2-VO2/FTO thin films synthesized by sonochemistry
  11. Macroscopic analysis of chemical looping combustion with ilmenite versus conventional oxides as oxygen carriers
  12. Robust control designs for microalgae cultivation in continuous photobioreactors
  13. Evaluation of a rough-surface evaporator applied to an absorption heat transformer for water desalination
  14. Optimized infrared-assisted extraction to obtain total lipid from microalgae Scenedesmus obliquus: a green approach
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