Abstract
Fructofuranosidase (EC 3.2.1.26) from Aureobasidium sp. ATCC 20524, recovered from 5 L fermented medium, purified by two simple steps with a yield of 65 % and a purification factor of 16, was immobilized by adsorption onto titanium dioxide (FTIO). The enzyme was also covalently immobilized onto TiO2 coated with polyethyleneimine (FTIOP) and encapsulated in gellan gum (FTIOPG). FTIO and FTIOP recorded an activity of 903 U g−1 and 9212 U g−1, respectively. The immobilized enzyme showed high activity and stability at pH levels ranging from 4.0 to 8.0 and there were no changes in the temperature profile for either methodology when compared with free fructofuranosidase. The immobilized biocatalysts were reused 7 times for FOS production without significant activity loss, except FTIO at pH 5.0. Gellan gum was used for FTIOP encapsulation. FOS production was performed in a batch and a continuous reactor using FTIOPG as a biocatalyst. Batch conversion (gFOS/ginitial sucrose) was around 60 % for initial sucrose concentrations of 100, 300 and 600 g L−1, at a time of maximum conversion. Fixed-bed reactor operational stability was remarkable, providing a constant FOS production in the outlet of the column during 720 h.
Acknowledgements
This work was supported by CONICET, Consejo de Investigaciones de la Universidad Nacional de Tucumán (PIUNT 26/E531, 26/E440), COFECyT, Consejo de Investigación de la Universidad Nacional de Salta (PI 2009 and PI 2174) and Ministerio de Educación de Argentina. We acknowledge a doctoral fellowship from CONICET given to Daniel H. Valdeón. Authors also thank Evonik Degussa Argentina for kindly providing Aeroxide TiO2.
References
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Articles in the same Issue
- Impact of Activation Energy in Nonlinear Mixed Convective Chemically Reactive Flow of Third Grade Nanomaterial by a Rotating Disk
- A Study on the Role of Clostridium Saccharoperbutylacetonicum N1-4 (ATCC 13564) in Producing Fermentative Hydrogen
- Pilot-Scale Study on Improving SNCR Denitrification Efficiency by Using Gas Additives
- Synthesis and Optimization of Methyl Laurate Using Sulfonated Pyrrolidonium Ionic Liquid as a Catalyst
- Hydrodynamics Modeling of an LSCFB Reactor Using Multigene Genetic Programming Approach: Effect of Particles Size and Shape
- Immobilization of Fructofuranosidase from Aureobasidium sp. Onto TiO2 and Its Encapsulation on Gellan Gum for FOS Production
- Potassium Hydroxide Activated Hydrogen Generation Using Aluminum in Water Splitting Reaction
- Arrhenius Activation Energy Impact in Binary Chemically Reactive Flow of TiO2-Cu- H2O Hybrid Nanomaterial
- Gas-Phase Mercury Removal by Modified Activated Carbons Treated with Ar-O2 Non-Thermal Plasma under Different O2 Concentrations
- Impact of Thermal Asymmetry on Efficiency of the Heat Recovery and Ways of Restoring Symmetry in the Flow Reversal Reactors
Articles in the same Issue
- Impact of Activation Energy in Nonlinear Mixed Convective Chemically Reactive Flow of Third Grade Nanomaterial by a Rotating Disk
- A Study on the Role of Clostridium Saccharoperbutylacetonicum N1-4 (ATCC 13564) in Producing Fermentative Hydrogen
- Pilot-Scale Study on Improving SNCR Denitrification Efficiency by Using Gas Additives
- Synthesis and Optimization of Methyl Laurate Using Sulfonated Pyrrolidonium Ionic Liquid as a Catalyst
- Hydrodynamics Modeling of an LSCFB Reactor Using Multigene Genetic Programming Approach: Effect of Particles Size and Shape
- Immobilization of Fructofuranosidase from Aureobasidium sp. Onto TiO2 and Its Encapsulation on Gellan Gum for FOS Production
- Potassium Hydroxide Activated Hydrogen Generation Using Aluminum in Water Splitting Reaction
- Arrhenius Activation Energy Impact in Binary Chemically Reactive Flow of TiO2-Cu- H2O Hybrid Nanomaterial
- Gas-Phase Mercury Removal by Modified Activated Carbons Treated with Ar-O2 Non-Thermal Plasma under Different O2 Concentrations
- Impact of Thermal Asymmetry on Efficiency of the Heat Recovery and Ways of Restoring Symmetry in the Flow Reversal Reactors