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Adsorption Properties of Arc Produced Multi Walled Carbon Nanotubes for Bovine Serum Albumin

  • Kadriye Bozgeyik and Turkan Kopac EMAIL logo
Published/Copyright: March 3, 2016
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International Journal of Chemical Reactor Engineering
From the journal International Journal of Chemical Reactor Engineering Volume 14 Issue 2

Abstract

In this study, adsorption properties of arc produced Multi Walled Carbon Nanotubes (MWNT) were investigated for Bovine Serum Albumin (BSA) in aqueous phase. Solution pH, adsorbent amount and temperature effects were examined on protein adsorption. The results show that, the temperature and the adsorbent amount both increase the BSA adsorption, whereas the solution pH has a decreasing effect. The equilibrium behavior of protein adsorption was examined by Langmuir and Freundlich isotherms. The monolayer adsorption capacities at 40 °C for solution pH 4 and 5 were determined as 139.5 and 127.2 mg g−1, respectively, which were much higher than the BSA adsorption capacities of various metal oxides investigated in our previous studies. The adsorption rate data were compared by the pseudo-first and the second-order kinetics equations. Evaluation of the experimental kinetics data have shown that the adsorption of BSA by MWNT followed the pseudo-first-order kinetics. The pseudo-first order adsorption rate constants at pH 4 and 5 decreased with an increase in temperature which results in a decrease in diffusion rate of BSA molecules across the external boundary layer, and favors the sorption process. The adsorption behavior of protein by carbon nanotubes was explained also using the zeta potential measurements. The adsorption capacity decreased with increasing pH due to the electrostatic repulsions. The thermodynamic parameters evaluated to predict the nature of adsorption confirmed the non-spontaneous and endothermic behaviour of the BSA/MWNT adsorption process. Adsorption standard enthalpy values were found as ∆H0=59.5 kJ mol−1 and ∆H0=14.3 kJ mol−1 for pH 4 and 5, respectively indicating that the protein molecules are adsorbed electrostatically on the carbon nanotubes.

Keywords: Bovine serum albumin; protein adsorption; multi walled carbon nanotubes; zeta potential

Funding source: Bülent Ecevit University, Zonguldak, Turkey

Award Identifier / Grant number: BEUN Scientific Research Project 2010-13-02-09

Funding statement: The authors acknowledge the financial support from Bülent Ecevit University, Zonguldak, Turkey (BEUN Scientific Research Project 2010-13-02-09).

Nomenclature

b

Langmuir model constant, related to the affinity of the binding sites, dm3/mg

C

BSA concentration in liquid-phase at time t, mg/L

C0

initial liquid-phase BSA concentration, mg/L

Ce

equilibrium liquid-phase concentration of BSA, mg/L

Kf

Freundlich model constant

k1

pseudo-first order adsorption rate constant, min−1

k2

pseudo-second order adsorption rate constant, g mg−1 min−1

mads

adsorbent mass, g

1/n

exponent in Freundlich model

q

BSA concentration in solid-phase at time t, mg/g

qe

equilibrium solid-phase concentration, mg/g

R

Universal gas constant (8.314 J mol−1 K−1)

r2

coefficient of correlation

Q0

Langmuir equilibrium constant (mg/g)

t

time, min

T

temperature, °C

V

volume of solution, L

∆G0

standard free energy change (J/mol)

∆H0

standard entalphy change (J/mol)

∆S0

standard entropy change (J/mol K)

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Published Online: 2016-3-3
Published in Print: 2016-4-1

©2016 by De Gruyter

Articles in the same Issue

  1. Frontmatter
  2. Research Articles
  3. Adsorption Properties of Arc Produced Multi Walled Carbon Nanotubes for Bovine Serum Albumin
  4. Experimental Study and Mathematical Modeling of Propane-SCR-NOx Using Group Method of Data Handling and Artificial Neural Network
  5. Experimental and Kinetic Study of Esterification of Acrylic Acid with Ethanol Using Homogeneous Catalyst
  6. Synthesis of Butyl Acetate in a Membrane Reactor in a Flow-Through Mode
  7. Heat Transfer Enhancement Around a Cylinder – A CFD Study of Effect of Corner Radius and Prandtl Number
  8. CFD Modeling with Experimental Validation of the Internal Hydrodynamics in a Pilot-Scale Slurry Bubble Column Reactor
  9. Computational Simulation of Mixing Performance in the Circulating Jet Mixing Tank
  10. In Situ Gasification Chemical Looping Combustion of Coal Using the Mixed Oxygen Carrier of Natural Anhydrite Ore and Calcined Limestone
  11. Effect of L/D Ratio on Phase Holdup and Bubble Dynamics in Slurry Bubble Column using Optical Fiber Probe Measurements
https://doi.org/10.1515/ijcre-2015-0160
Keywords for this article
Bovine serum albumin; protein adsorption; multi walled carbon nanotubes; zeta potential

Articles in the same Issue

  1. Frontmatter
  2. Research Articles
  3. Adsorption Properties of Arc Produced Multi Walled Carbon Nanotubes for Bovine Serum Albumin
  4. Experimental Study and Mathematical Modeling of Propane-SCR-NOx Using Group Method of Data Handling and Artificial Neural Network
  5. Experimental and Kinetic Study of Esterification of Acrylic Acid with Ethanol Using Homogeneous Catalyst
  6. Synthesis of Butyl Acetate in a Membrane Reactor in a Flow-Through Mode
  7. Heat Transfer Enhancement Around a Cylinder – A CFD Study of Effect of Corner Radius and Prandtl Number
  8. CFD Modeling with Experimental Validation of the Internal Hydrodynamics in a Pilot-Scale Slurry Bubble Column Reactor
  9. Computational Simulation of Mixing Performance in the Circulating Jet Mixing Tank
  10. In Situ Gasification Chemical Looping Combustion of Coal Using the Mixed Oxygen Carrier of Natural Anhydrite Ore and Calcined Limestone
  11. Effect of L/D Ratio on Phase Holdup and Bubble Dynamics in Slurry Bubble Column using Optical Fiber Probe Measurements
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