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Carnosic acid prevents heat stress-induced oxidative damage by regulating heat-shock proteins and apoptotic proteins in mouse testis

  • Sirui Liu ORCID logo , Jiaxin Wu , Wanqing Liang , Yinkun Liu , Shuangshuang Wan and Shu Tang EMAIL logo
Published/Copyright: December 6, 2024
Biological Chemistry
From the journal Biological Chemistry Volume 405 Issue 11-12

Abstract

Heat stress impacts male reproduction in animal husbandry. Carnosic acid (CA), a potent antioxidant, mitigates oxidative stress and apoptosis. αB-crystallin, a small heat shock protein, regulates apoptosis and oxidative stress. This study examines the protective effects of CA on the testis in wild-type and αB-crystallin knockout mice under heat stress. CA pretreatment increased testosterone levels and preserved testicular structure in wild-type mice, but no changes in knockout mice. CA reduced Hsp27, Hsp70, and cleaved caspase-3 levels, while knockout mice showed increased cleaved caspase-3. These results suggest that CA protects the testis by modulating heat shock and apoptosis-related proteins.

Keywords: carnosic acid; Hsp70; heat stress; apoptosis; knockout mice
Corresponding author: Shu Tang, College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China, E-mail:

Funding source: the National Key R&D Program of Ningxia Hui Autonomous Region of China

Award Identifier / Grant number: 21BEF02019

Funding source: the National Natural Science Foundation of China

Award Identifier / Grant number: 31602027

Funding source: the Natural Science Foundation of Ningxia Province

Award Identifier / Grant number: 2023AAC05052

  1. Research ethics: All experiments were performed in accordance with the guidelines of the Animal Ethics Committee of Jiangsu Province (China) and were approved by the Institutional Animal Care and Use Committee of Nanjing Agricultural University, China.

  2. Author contributions: The authors have accepted responsibility for the entire content of this manuscript and approved its submission. Conceptualization, Sirui Liu; Data curation, Yinkun Liu; Investigation, Sirui Liu and Jiaxin Wu; Methodology, Sirui Liu; Software, Jiaxin Wu; Supervision, Shu Tang; Validation, Shuangshuang Wan; Visualization, Wanqing Liang; Writing – original draft, Sirui Liu; Writing – review & editing, Shu Tang.

  3. Use of Large Language Models, AI and Machine Learning Tools: None declared.

  4. Conflict of interest: The authors state no conflict of interest.

  5. Research funding: This work was supported by the Natural Science Foundation of Ningxia Province [grant number 2023AAC05052]; the National Key R&D Program of Ningxia Hui Autonomous Region of China [grant number 21BEF02019]; and the National Natural Science Foundation of China [grant number 31602027].

  6. Data availability: The raw data can be obtained on request from the corresponding author.

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Received: 2023-12-20
Accepted: 2024-11-25
Published Online: 2024-12-06
Published in Print: 2024-12-17

© 2024 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/hsz-2023-0374
Keywords for this article
carnosic acid; Hsp70; heat stress; apoptosis; knockout mice

Articles in the same Issue

  1. Frontmatter
  2. Review
  3. Structure, function, and recombinant production of EGFL7
  4. Research Articles/Short Communications
  5. Protein Structure and Function
  6. Zinc and copper effect mechanical cell adhesion properties of the amyloid precursor protein
  7. The BCL11A transcription factor stimulates the enzymatic activities of the OGG1 DNA glycosylase
  8. Cell Biology and Signaling
  9. Bioprospecting hydroxylated chalcones in in vitro model of ischemia-reoxygenation and probing NOX4 interactions via molecular docking
  10. Carnosic acid prevents heat stress-induced oxidative damage by regulating heat-shock proteins and apoptotic proteins in mouse testis
  11. Novel Techniques
  12. Simultaneous spectral illumination of microplates for high-throughput optogenetics and photobiology
  13. A platform for the early selection of non-competitive antibody-fragments from yeast surface display libraries
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