Startseite In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L.
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In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L.

  • Minh Thi Hong Nguyen EMAIL logo , Chien Van Tran , Phuong Hong Nguyen , Quang De Tran , Min-Sung Kim , Won-Kyo Jung und Phuong Thi Mai Nguyen EMAIL logo
Veröffentlicht/Copyright: 3. August 2021
Biological Chemistry
Aus der Zeitschrift Biological Chemistry Band 402 Heft 10

Abstract

Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population. Therefore, finding the effective osteogenic compounds for the treatment of osteoporosis is a promising research approach. In our study, tamarind (Tamarindus indica L.) seed polysaccharide (TSP) extracted from tamarind seed was subjected to synthesize its sulfate derivatives. The 1H NMR, FT-IR, SEM, monosaccharide compositions and elemental analysis data revealed that tamarind seed polysaccharide sulfate (TSPS) was successfully prepared. As the result, TSPS showed potent effects on inducing osteoblast differentiation via increasing alkaline phosphatase (ALP) activity up to 20% after 10 days and bone mineralization approximately 58% after four weeks at concentration of 20 μg/mL, whereas no statistically increase for both ALP activity and bone mineralization was observed in TSP treatment. Furthermore, TSPS enhanced expression of several marker genes in bone formation. Overall, the obtained data provided novelty on osteogenic compounds originated from TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases.

Keywords: bone formation; osteoblast differentiation; sulfated polysaccharide; tamarind seed polysaccharide; Tamarindus indica
Corresponding authors: Minh Thi Hong Nguyen, University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Hanoi, 100000, Vietnam; and Phuong Thi Mai Nguyen, Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Hanoi, 100000, Vietnam; and Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Hanoi, 100000, Vietnam, E-mail: (M. T. H. Nguyen), (P. T. M. Nguyen)

Funding source: Vietnam National Foundation for Science and Technology Development (NAFOSTED)

Award Identifier / Grant number: 106.02-2018.24

Acknowledgments

The authors thank Nguyen Mai Huong for the RT-PCR experiment, Nguyen Luong Lam for FT-IR experiment, and Ngo Trong Hieu for helping in TSPS synthesis.

  1. Author contributions: Minh T.H. Nguyen designed the project, supervised, performed the experiments, analyzed data, and wrote the manuscript. Chien Van Tran performed the experiments of TSPS synthesis, analyzed data, and wrote the manuscript. Phuong Hong Nguyen and Quang De Tran performed the experiments of elemental analysis, and wrote the manuscript. Min-Sung Kim performed SEM analysis and edited the manuscript. Won-Kyo Jung supervised the experiments and edited the manuscript. Phuong T.M. Nguyen designed the project, supervised, and wrote the manuscript. All authors have read and approved the final manuscript.

  2. Research funding: This research is funded by Vietnam National Foundation for Science and Technology Development (NAFOSTED) under grant number 106.02-2018.24.

  3. Conflict of interest statement: The authors declare no conflicts of interest regarding this article.

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Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/hsz-2021-0200).

Received: 2021-03-22
Accepted: 2021-07-01
Published Online: 2021-08-03
Published in Print: 2021-09-27

© 2021 Walter de Gruyter GmbH, Berlin/Boston

Artikel in diesem Heft

  1. Frontmatter
  2. Review
  3. Biophysical applications in structural and molecular biology
  4. Research Articles/Short Communications
  5. Genes and Nucleic Acids
  6. Historic nucleic acids isolated by Friedrich Miescher contain RNA besides DNA
  7. Protein Structure and Function
  8. The extracellular region of bovine milk butyrophilin exhibits closer structural similarity to human myelin oligodendrocyte glycoprotein than to immunological BTN family receptors
  9. Structural insights into the repair mechanism of AGT for methyl-induced DNA damage
  10. Cell Biology and Signaling
  11. In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L.
  12. Synthesis and biological characterization of a new fluorescent probe for vesicular trafficking based on polyazamacrocycle derivative
  13. Properties of transmembrane helix TM1 of the DcuS sensor kinase of Escherichia coli, the stator for TM2 piston signaling
  14. Lefty A is involved in sunitinib resistance of renal cell carcinoma cells via regulation of IL-8
  15. Proteolysis
  16. Tissue factor pathway inhibitor 2 is a potent kallikrein-related protease 12 inhibitor
https://doi.org/10.1515/hsz-2021-0200
Schlagwörter für diesen Artikel
bone formation; osteoblast differentiation; sulfated polysaccharide; tamarind seed polysaccharide; Tamarindus indica

Artikel in diesem Heft

  1. Frontmatter
  2. Review
  3. Biophysical applications in structural and molecular biology
  4. Research Articles/Short Communications
  5. Genes and Nucleic Acids
  6. Historic nucleic acids isolated by Friedrich Miescher contain RNA besides DNA
  7. Protein Structure and Function
  8. The extracellular region of bovine milk butyrophilin exhibits closer structural similarity to human myelin oligodendrocyte glycoprotein than to immunological BTN family receptors
  9. Structural insights into the repair mechanism of AGT for methyl-induced DNA damage
  10. Cell Biology and Signaling
  11. In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L.
  12. Synthesis and biological characterization of a new fluorescent probe for vesicular trafficking based on polyazamacrocycle derivative
  13. Properties of transmembrane helix TM1 of the DcuS sensor kinase of Escherichia coli, the stator for TM2 piston signaling
  14. Lefty A is involved in sunitinib resistance of renal cell carcinoma cells via regulation of IL-8
  15. Proteolysis
  16. Tissue factor pathway inhibitor 2 is a potent kallikrein-related protease 12 inhibitor
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