Abstract
Synaptic plasticity underlies central brain functions, such as learning. Ca2+ signaling is involved in both strengthening and weakening of synapses, but it is still unclear how one signal molecule can induce two opposite outcomes. By identifying molecules, which can distinguish between signaling leading to weakening or strengthening, we can improve our understanding of how synaptic plasticity is regulated. Here, we tested gelsolin’s response to the induction of chemical long-term potentiation (cLTP) or long-term depression (cLTD) in cultured rat hippocampal neurons. We show that gelsolin relocates from the dendritic shaft to dendritic spines upon cLTD induction while it did not show any relocalization upon cLTP induction. Dendritic spines are small actin-rich protrusions on dendrites, where LTD/LTP-responsive excitatory synapses are located. We propose that the LTD-induced modest – but relatively long-lasting – elevation of Ca2+ concentration increases the affinity of gelsolin to F-actin. As F-actin is enriched in dendritic spines, it is probable that increased affinity to F-actin induces the relocalization of gelsolin.
Acknowledgments
We thank Seija Lågas, Outi Nikkilä, Rimante Minkeviciene and Amr Abouelezz for their help with neuronal culture preparation. We are grateful to Amr Abouelezz for the English proofreading. All imaging was performed using microscopes in the Biomedicum Imaging Unit of University of Helsinki. This work was supported by the CIMO (IH/PH), Kordelin Foundation (IH), Minerva Foundation (IH/PH), Instrumentarium Foundation (PH), Academy of Finland (PH, SA 266351).
Conflict of interest statement: The authors declare no competing financial interests.
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© 2019 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Highlight: dynamics of the nervous system in health and disease
- Actin(g) on mitochondria – a role for cofilin1 in neuronal cell death pathways
- Neuronal microtubules and proteins linked to Parkinson’s disease: a relevant interaction?
- Contribution of astrocytes to metabolic dysfunction in the Alzheimer’s disease brain
- Chemical LTD, but not LTP, induces transient accumulation of gelsolin in dendritic spines
- Sub-membranous actin rings in the axon initial segment are resistant to the action of latrunculin
- The role of GFAP and vimentin in learning and memory
- Vimentin is required for normal accumulation of body fat
- The microtubule skeleton and the evolution of neuronal complexity in vertebrates
- Amyloidosis causes downregulation of SorLA, SorCS1 and SorCS3 expression in mice
- A fluorescent protein-readout for transcriptional activity reveals regulation of APP nuclear signaling by phosphorylation sites
- Kavalactones from Kava (Piper methysticum) root extract as modulators of recombinant human glycine receptors
- Research Articles/Short Communications
- Cell Biology and Signaling
- LncRNA HOTAIR targets miR-126-5p to promote the progression of Parkinson’s disease through RAB3IP
- Novel Techniques
- In vitro import experiments with semi-intact cells suggest a role of the Sec61 paralog Ssh1 in mitochondrial biogenesis
- Corrigendum
- Corrigendum to: SIAH1/ZEB1/IL-6 axis is involved in doxorubicin (Dox) resistance of osteosarcoma cells
Artikel in diesem Heft
- Frontmatter
- Highlight: dynamics of the nervous system in health and disease
- Actin(g) on mitochondria – a role for cofilin1 in neuronal cell death pathways
- Neuronal microtubules and proteins linked to Parkinson’s disease: a relevant interaction?
- Contribution of astrocytes to metabolic dysfunction in the Alzheimer’s disease brain
- Chemical LTD, but not LTP, induces transient accumulation of gelsolin in dendritic spines
- Sub-membranous actin rings in the axon initial segment are resistant to the action of latrunculin
- The role of GFAP and vimentin in learning and memory
- Vimentin is required for normal accumulation of body fat
- The microtubule skeleton and the evolution of neuronal complexity in vertebrates
- Amyloidosis causes downregulation of SorLA, SorCS1 and SorCS3 expression in mice
- A fluorescent protein-readout for transcriptional activity reveals regulation of APP nuclear signaling by phosphorylation sites
- Kavalactones from Kava (Piper methysticum) root extract as modulators of recombinant human glycine receptors
- Research Articles/Short Communications
- Cell Biology and Signaling
- LncRNA HOTAIR targets miR-126-5p to promote the progression of Parkinson’s disease through RAB3IP
- Novel Techniques
- In vitro import experiments with semi-intact cells suggest a role of the Sec61 paralog Ssh1 in mitochondrial biogenesis
- Corrigendum
- Corrigendum to: SIAH1/ZEB1/IL-6 axis is involved in doxorubicin (Dox) resistance of osteosarcoma cells