Abstract
Vitamin D3 is known to have anticancer actions by affecting tumorigenesis including the cell cycle and cell apoptosis in gastric cancer (GC) cells; the genes including microRNAs (miRNAs) regulated by vitamin D3 signaling remain discovered. miR-99b-3p, the tumor suppressor gene, is not only decreased in GC tissues, but is also induced by vitamin D3 through the vitamin D receptor (VDR) binding on the promoter domain of miR-99b. Further study indicates that miR-99b-3p inhibits cell viability and induces cell arrest in the S-phase in GC cells, the direct target gene of miR-99b-3p is verified to be HoxD3, which is also overexpressed in GC cell lines. Overall, our results show that miR-99b-3p mediates the antiproliferative of vitamin D3 in GC cells and might hold promise for prognosis and therapeutic strategies for GC treatment.
Funding source: National Natural Science Foundation of China
Award Identifier / Grant number: 81601081
Funding source: China Postdoctoral Science Foundation
Award Identifier / Grant number: 2017M623195
Funding statement: This work was supported by The National Natural Science Foundation of China (no. 81601081); The Fundamental Research Funds for the Central Universities (no. XGG2017015); The China Postdoctoral Science Foundation (no. 2017M623195).
Conflict of interest statement: The authors declare no conflict of interest.
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© 2019 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Reviews
- The multifaceted roles of tumor-associated proteases and harnessing their activity for prodrug activation
- Retrograde response by reactive oxygen/nitrogen species in plants involving different cellular organelles
- Molecular effects of dietary fatty acids on brain insulin action and mitochondrial function
- STRIPAK, a highly conserved signaling complex, controls multiple eukaryotic cellular and developmental processes and is linked with human diseases
- Research Articles/Short Communications
- Cell Biology and Signaling
- Zinc-induced activation of GPR39 regulates glucose homeostasis through glucose-dependent insulinotropic polypeptide secretion from enteroendocrine K-cells
- Long non-coding RNA CHRF promotes proliferation and mesenchymal transition (EMT) in prostate cancer cell line PC3 requiring up-regulating microRNA-10b
- LncRNA DYNLRB2-2 inhibits THP-1 macrophage foam cell formation by enhancing autophagy
- Geniposide alleviates lipopolysaccharide (LPS)-induced inflammation by downregulation of miR-27a in rat pancreatic acinar cell AR42J
- Tripterine inhibits proliferation, migration and invasion of breast cancer MDA-MB-231 cells by up-regulating microRNA-15a
- miR-99b-3p is induced by vitamin D3 and contributes to its antiproliferative effects in gastric cancer cells by targeting HoxD3
Articles in the same Issue
- Frontmatter
- Reviews
- The multifaceted roles of tumor-associated proteases and harnessing their activity for prodrug activation
- Retrograde response by reactive oxygen/nitrogen species in plants involving different cellular organelles
- Molecular effects of dietary fatty acids on brain insulin action and mitochondrial function
- STRIPAK, a highly conserved signaling complex, controls multiple eukaryotic cellular and developmental processes and is linked with human diseases
- Research Articles/Short Communications
- Cell Biology and Signaling
- Zinc-induced activation of GPR39 regulates glucose homeostasis through glucose-dependent insulinotropic polypeptide secretion from enteroendocrine K-cells
- Long non-coding RNA CHRF promotes proliferation and mesenchymal transition (EMT) in prostate cancer cell line PC3 requiring up-regulating microRNA-10b
- LncRNA DYNLRB2-2 inhibits THP-1 macrophage foam cell formation by enhancing autophagy
- Geniposide alleviates lipopolysaccharide (LPS)-induced inflammation by downregulation of miR-27a in rat pancreatic acinar cell AR42J
- Tripterine inhibits proliferation, migration and invasion of breast cancer MDA-MB-231 cells by up-regulating microRNA-15a
- miR-99b-3p is induced by vitamin D3 and contributes to its antiproliferative effects in gastric cancer cells by targeting HoxD3