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miR-99b-3p is induced by vitamin D3 and contributes to its antiproliferative effects in gastric cancer cells by targeting HoxD3

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Published/Copyright: July 9, 2019

Abstract

Vitamin D3 is known to have anticancer actions by affecting tumorigenesis including the cell cycle and cell apoptosis in gastric cancer (GC) cells; the genes including microRNAs (miRNAs) regulated by vitamin D3 signaling remain discovered. miR-99b-3p, the tumor suppressor gene, is not only decreased in GC tissues, but is also induced by vitamin D3 through the vitamin D receptor (VDR) binding on the promoter domain of miR-99b. Further study indicates that miR-99b-3p inhibits cell viability and induces cell arrest in the S-phase in GC cells, the direct target gene of miR-99b-3p is verified to be HoxD3, which is also overexpressed in GC cell lines. Overall, our results show that miR-99b-3p mediates the antiproliferative of vitamin D3 in GC cells and might hold promise for prognosis and therapeutic strategies for GC treatment.

Award Identifier / Grant number: 81601081

Award Identifier / Grant number: 2017M623195

Funding statement: This work was supported by The National Natural Science Foundation of China (no. 81601081); The Fundamental Research Funds for the Central Universities (no. XGG2017015); The China Postdoctoral Science Foundation (no. 2017M623195).

  1. Conflict of interest statement: The authors declare no conflict of interest.

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Received: 2019-01-03
Accepted: 2019-04-17
Published Online: 2019-07-09
Published in Print: 2019-07-26

© 2019 Walter de Gruyter GmbH, Berlin/Boston

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