Abstract
Parkinson’s disease (PD) is a common neurological disorder characterized by dopaminergic (DA) neuron degeneration and death in the midbrain, and the long noncoding RNA HOTAIR has been shown to affect disease progression in PD. In this study, we aimed to further illustrate the molecular mechanism of HOTAIR in PD. Bioinformatics analysis was utilized to determine the potential downstream targets of HOTAIR in PD. Luciferase assay and the RNA Binding Protein Immunoprecipitation (RIP) assay were used to validate the existence of binding sites between competing endogenous RNAs (ceRNAs). Real-time quantitative polymerase chain reaction (qRT-PCR) and Western blotting indicated that HOTAIR and RAB3IP increased while miR-126-5p decreased in PD cells and PD mice. Additionally, the CCK-8 assay and flow cytometric analysis indicated that the knockdown of HOTAIR and RAB3IP and the overexpression of miR-126-5p significantly increased cell proliferation and reduced apoptosis in PD cells. Furthermore, the results of in vivo experiments suggested that knockdown of HOTAIR expression increased the number of TH-positive cells and the number of α-synuclein-positive cells decreased while reducing the apoptosis rate among DA neurons. Our study confirmed that HOTAIR promotes PD progression by regulating miR-126-5p and RAB3IP in a ceRNA-dependent manner and further clarified how HOTAIR works in PD.
Conflict of interest statement: The authors declare that they have no conflicts of interest regarding this work.
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Supplementary Material
The online version of this article offers supplementary material (https://doi.org/10.1515/hsz-2018-0431).
© 2019 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Highlight: dynamics of the nervous system in health and disease
- Actin(g) on mitochondria – a role for cofilin1 in neuronal cell death pathways
- Neuronal microtubules and proteins linked to Parkinson’s disease: a relevant interaction?
- Contribution of astrocytes to metabolic dysfunction in the Alzheimer’s disease brain
- Chemical LTD, but not LTP, induces transient accumulation of gelsolin in dendritic spines
- Sub-membranous actin rings in the axon initial segment are resistant to the action of latrunculin
- The role of GFAP and vimentin in learning and memory
- Vimentin is required for normal accumulation of body fat
- The microtubule skeleton and the evolution of neuronal complexity in vertebrates
- Amyloidosis causes downregulation of SorLA, SorCS1 and SorCS3 expression in mice
- A fluorescent protein-readout for transcriptional activity reveals regulation of APP nuclear signaling by phosphorylation sites
- Kavalactones from Kava (Piper methysticum) root extract as modulators of recombinant human glycine receptors
- Research Articles/Short Communications
- Cell Biology and Signaling
- LncRNA HOTAIR targets miR-126-5p to promote the progression of Parkinson’s disease through RAB3IP
- Novel Techniques
- In vitro import experiments with semi-intact cells suggest a role of the Sec61 paralog Ssh1 in mitochondrial biogenesis
- Corrigendum
- Corrigendum to: SIAH1/ZEB1/IL-6 axis is involved in doxorubicin (Dox) resistance of osteosarcoma cells
Articles in the same Issue
- Frontmatter
- Highlight: dynamics of the nervous system in health and disease
- Actin(g) on mitochondria – a role for cofilin1 in neuronal cell death pathways
- Neuronal microtubules and proteins linked to Parkinson’s disease: a relevant interaction?
- Contribution of astrocytes to metabolic dysfunction in the Alzheimer’s disease brain
- Chemical LTD, but not LTP, induces transient accumulation of gelsolin in dendritic spines
- Sub-membranous actin rings in the axon initial segment are resistant to the action of latrunculin
- The role of GFAP and vimentin in learning and memory
- Vimentin is required for normal accumulation of body fat
- The microtubule skeleton and the evolution of neuronal complexity in vertebrates
- Amyloidosis causes downregulation of SorLA, SorCS1 and SorCS3 expression in mice
- A fluorescent protein-readout for transcriptional activity reveals regulation of APP nuclear signaling by phosphorylation sites
- Kavalactones from Kava (Piper methysticum) root extract as modulators of recombinant human glycine receptors
- Research Articles/Short Communications
- Cell Biology and Signaling
- LncRNA HOTAIR targets miR-126-5p to promote the progression of Parkinson’s disease through RAB3IP
- Novel Techniques
- In vitro import experiments with semi-intact cells suggest a role of the Sec61 paralog Ssh1 in mitochondrial biogenesis
- Corrigendum
- Corrigendum to: SIAH1/ZEB1/IL-6 axis is involved in doxorubicin (Dox) resistance of osteosarcoma cells