Silencing of MED27 inhibits adrenal cortical carcinogenesis by targeting the Wnt/β-catenin signaling pathway and the epithelial-mesenchymal transition process
Abstract
This study aimed to explore the effect of MED27 on the expression of epithelial-mesenchymal transition (EMT)-related proteins and β-catenin in adrenal cortical carcinoma (ACC). The functional mechanism of MED27 on ACC processes was also explored. The expression of MED27 was assessed by quantitative real-time polymerase chain reaction (qRT-PCR). siRNA was utilized to knockdown the expression of MED27. CCK8 assays were performed to evaluate SW-13 cell proliferation. Transwell assays were performed to assess the invasion ability, and wound healing assays were utilized to detect migration. A tumor xenograft mouse model was established to investigate the impact of silencing MED27 on tumor growth and metastasis. MED27 was highly expressed in ACC tissues and cells. Down-regulation of MED27 induced ACC cell apoptosis, and significantly attenuated ACC cell proliferation, invasion and metastasis in vivo and in vitro. MED27 knockdown regulated the expression of EMT-related proteins and Wnt/β-catenin signaling pathway-related proteins. Our study investigated the function and mechanism of MED27 and validated that MED27 plays a negative role in ACC occurrence and progression and could be utilized as a new therapeutic target in ACC prevention and treatment.
Funding: This study was supported by the Medical and Technology Intercrossing Research Foundation of Shanghai Jiaotong University (YG2016QN65).
References
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©2018 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Reviews
- Hodgkin lymphoma cell lines: to separate the wheat from the chaff
- The AGO proteins: an overview
- Research Articles/Short Communications
- Protein Structure and Function
- Structural changes at the myrtenol backbone reverse its positive allosteric potential into inhibitory GABAA receptor modulation
- The two major glucokinase isoforms show conserved functionality in β-cells despite different subcellular distribution
- Functional characterization of the mouse Serpina1 paralog DOM-7
- Cell Biology and Signaling
- CD45RO regulates the HIV-1 gp120-mediated apoptosis of T cells by activating Lck
- Silencing of MED27 inhibits adrenal cortical carcinogenesis by targeting the Wnt/β-catenin signaling pathway and the epithelial-mesenchymal transition process
- HDAC1 knockdown inhibits invasion and induces apoptosis in non-small cell lung cancer cells
- Hepatitis B virus X protein promotes proliferation of hepatocellular carcinoma cells by upregulating miR-181b by targeting ING5
Articles in the same Issue
- Frontmatter
- Reviews
- Hodgkin lymphoma cell lines: to separate the wheat from the chaff
- The AGO proteins: an overview
- Research Articles/Short Communications
- Protein Structure and Function
- Structural changes at the myrtenol backbone reverse its positive allosteric potential into inhibitory GABAA receptor modulation
- The two major glucokinase isoforms show conserved functionality in β-cells despite different subcellular distribution
- Functional characterization of the mouse Serpina1 paralog DOM-7
- Cell Biology and Signaling
- CD45RO regulates the HIV-1 gp120-mediated apoptosis of T cells by activating Lck
- Silencing of MED27 inhibits adrenal cortical carcinogenesis by targeting the Wnt/β-catenin signaling pathway and the epithelial-mesenchymal transition process
- HDAC1 knockdown inhibits invasion and induces apoptosis in non-small cell lung cancer cells
- Hepatitis B virus X protein promotes proliferation of hepatocellular carcinoma cells by upregulating miR-181b by targeting ING5