Production of recombinant porin from Y. pseudotuberculosis in a water-soluble form for pseudotuberculosis diagnostics
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Vasily Golotin
, Olga Portnyagina
, Natalia Chopenko , Natalia Kim , Valery Rasskazov und Olga Novikova
Abstract
OmpF porin from the outer membrane of Yersinia pseudotuberculosis was cloned into pET-40b(+) plasmid. Using E. coli Rosetta (DE3) strain, MX medium, IPTG concentration of 0.2 mm and post-induction cultivation at 14°C overnight allowed us to obtain a water-soluble form of the recombinant protein (rs-OmpF). Rs-OmpF was shown to have the ordered spatial structure at the levels of secondary and tertiary structure. Rs-OmpF was found to be effective as diagnostic antigen in ELISA for pseudotuberculosis diagnostics.
Acknowledgements
The work was partially supported by the grant 15-I-5-004 of Basic Research under FEB RAS program ‘Far East’ and also by the grant 15-15-00035 of Russian Science Foundation in the part of obtaining of the recombinant proteins.
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©2017 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Reviews
- Oxidised protein metabolism: recent insights
- Immune-regulation and -functions of eicosanoid lipid mediators
- Reactive nitrogen species (RNS)-resistant microbes: adaptation and medical implications
- Progress in understanding the molecular oxygen paradox – function of mitochondrial reactive oxygen species in cell signaling
- Research Articles/Short Communications
- Protein Structure and Function
- Production of recombinant porin from Y. pseudotuberculosis in a water-soluble form for pseudotuberculosis diagnostics
- Membranes, Lipids, Glycobiology
- Functional control of polypeptide GalNAc-transferase 3 through an acetylation site in the C-terminal lectin domain
- Cell Biology and Signaling
- Human U3 protein 14a plays an anti-apoptotic role in cancer cells
- Cellular and plasma nitrite levels in myeloid leukemia: a pathogenetic decrease
Artikel in diesem Heft
- Frontmatter
- Reviews
- Oxidised protein metabolism: recent insights
- Immune-regulation and -functions of eicosanoid lipid mediators
- Reactive nitrogen species (RNS)-resistant microbes: adaptation and medical implications
- Progress in understanding the molecular oxygen paradox – function of mitochondrial reactive oxygen species in cell signaling
- Research Articles/Short Communications
- Protein Structure and Function
- Production of recombinant porin from Y. pseudotuberculosis in a water-soluble form for pseudotuberculosis diagnostics
- Membranes, Lipids, Glycobiology
- Functional control of polypeptide GalNAc-transferase 3 through an acetylation site in the C-terminal lectin domain
- Cell Biology and Signaling
- Human U3 protein 14a plays an anti-apoptotic role in cancer cells
- Cellular and plasma nitrite levels in myeloid leukemia: a pathogenetic decrease