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Biophysical methods toolbox to study ABC exporter structure and function

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Veröffentlicht/Copyright: 10. Oktober 2016
Biological Chemistry
Aus der Zeitschrift Biological Chemistry Band 398 Heft 2

Abstract

ABC exporters are highly dynamic membrane proteins that span a huge spectrum of different conformations. A detailed integrated approach of cellular, biochemical and biophysical characterization of these ‘open’, ‘closed’ and other intermediate states is central to understanding their function. Almost 40 years after the discovery of the first ABC transporter, thanks to the enormous development in methodologies, a picture is slowly emerging to visualize how these fascinating molecules transport their substrates. This mini review summarizes some of the biophysical tools that have made a major impact in understanding the function of the ABC exporters.

Keywords: ABC transporter; biophysical methods; single particle cryo electron microscopy; X-ray crystallography; X-ray free electron laser

Funding source: Deutsche Forschungsgemeinschaft

Award Identifier / Grant number: GZ:SR 113/1-1

Funding statement: We gratefully acknowledge the generous financial support of the Deutsche Forschungsgemeinschaft (DFG Research Grant) (Grant/Award Number: ‘GZ:SR 113/1-1’) and the LOEWE program of the State of Hesse.

Acknowledgements

We gratefully acknowledge the generous financial support of the Deutsche Forschungsgemeinschaft (DFG Research Grant) (Grant/Award Number: ‘GZ:SR 113/1-1’) and the LOEWE program of the State of Hesse.

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Received: 2016-6-30
Accepted: 2016-10-5
Published Online: 2016-10-10
Published in Print: 2017-2-1

©2017 Walter de Gruyter GmbH, Berlin/Boston

Artikel in diesem Heft

  1. Frontmatter
  2. Guest Editorial
  3. Highlight: the transporter colloquium – spotlight on membrane proteins
  4. HIGHLIGHT: 10. TRANSPORT-COLLOQUIUM OF THE GBM STUDY SECTION ‘BIOMEMBRANES’
  5. Transmitting the energy: interdomain cross-talk in Pdr5
  6. Stoichiometry determination of macromolecular membrane protein complexes
  7. The novel class of seven transmembrane segment inverted repeat carriers
  8. Expression, regulation and function of intestinal drug transporters: an update
  9. Guardians in a stressful world: the Opu family of compatible solute transporters from Bacillus subtilis
  10. Control of membrane fluidity: the OLE pathway in focus
  11. Biophysical methods toolbox to study ABC exporter structure and function
  12. Tropane alkaloids as substrates and inhibitors of human organic cation transporters of the SLC22 (OCT) and the SLC47 (MATE) families
  13. Strong pH dependence of coupling efficiency of the Na+ – translocating NADH:quinone oxidoreductase (Na+-NQR) of Vibrio cholerae
  14. Review
  15. Glutathione and glutathione derivatives in immunotherapy
  16. Research Articles/Short Communications
  17. Molecular Medicine
  18. E-beam crosslinked nanogels conjugated with monoclonal antibodies in targeting strategies
https://doi.org/10.1515/hsz-2016-0244
Schlagwörter für diesen Artikel
ABC transporter; biophysical methods; single particle cryo electron microscopy; X-ray crystallography; X-ray free electron laser

Artikel in diesem Heft

  1. Frontmatter
  2. Guest Editorial
  3. Highlight: the transporter colloquium – spotlight on membrane proteins
  4. HIGHLIGHT: 10. TRANSPORT-COLLOQUIUM OF THE GBM STUDY SECTION ‘BIOMEMBRANES’
  5. Transmitting the energy: interdomain cross-talk in Pdr5
  6. Stoichiometry determination of macromolecular membrane protein complexes
  7. The novel class of seven transmembrane segment inverted repeat carriers
  8. Expression, regulation and function of intestinal drug transporters: an update
  9. Guardians in a stressful world: the Opu family of compatible solute transporters from Bacillus subtilis
  10. Control of membrane fluidity: the OLE pathway in focus
  11. Biophysical methods toolbox to study ABC exporter structure and function
  12. Tropane alkaloids as substrates and inhibitors of human organic cation transporters of the SLC22 (OCT) and the SLC47 (MATE) families
  13. Strong pH dependence of coupling efficiency of the Na+ – translocating NADH:quinone oxidoreductase (Na+-NQR) of Vibrio cholerae
  14. Review
  15. Glutathione and glutathione derivatives in immunotherapy
  16. Research Articles/Short Communications
  17. Molecular Medicine
  18. E-beam crosslinked nanogels conjugated with monoclonal antibodies in targeting strategies
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