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Pokemon decreases the transcriptional activity of RARα in the absence of ligand

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Published/Copyright: September 19, 2016
Biological Chemistry
From the journal Biological Chemistry Volume 398 Issue 3

Abstract

Pokemon is a transcriptional repressor that belongs to the POZ and Krüppel (POK) protein family. In this study, we investigated the potential interaction between Pokemon and retinoic acid receptor alpha (RARα) and determined the role of Pokemon in regulation of RARα transcriptional activity in the absence of ligand. We found that Pokemon could directly interact with RARα. Moreover, we demonstrated that Pokemon could decrease the transcriptional activity of RARα in the absence of ligand. Furthermore, we showed that Pokemon could repress the transcriptional activity of RARα by increasing the recruitment of nuclear receptor co-repressor (NCoR) and silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) to the retinoic acid response element (RARE) element. Taken together, these data suggest that Pokemon is a novel partner of RARα that acts as a co-repressor to regulate RARα transcriptional activity in the absence of ligand.

Keywords: interaction; Pokemon; RARα; transcriptional activity

Funding source: National Natural Science Foundation of China

Award Identifier / Grant number: 31271154

Award Identifier / Grant number: 31171032

Funding statement: We thank Dr Underhill TM for kindly providing RARE-Luc plasmid. This work was supported by the grant from the National Natural Science Foundation of China (31271154, 31171032), the Beijing Natural Science Foundation (7162016), the Scientific Research Common Program of Beijing Municipal Commission of Education (KM201310025001), and the Basic Clinical Medical Research Cooperation Project of Capital Medical University (15JL52).

Acknowledgments

We thank Dr Underhill TM for kindly providing RARE-Luc plasmid. This work was supported by the grant from the National Natural Science Foundation of China (31271154, 31171032), the Beijing Natural Science Foundation (7162016), the Scientific Research Common Program of Beijing Municipal Commission of Education (KM201310025001), and the Basic Clinical Medical Research Cooperation Project of Capital Medical University (15JL52).

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Supplemental Material:

The online version of this article (DOI: 10.1515/hsz-2016-0142) offers supplementary material, available to authorized users.

Received: 2016-2-21
Accepted: 2016-9-14
Published Online: 2016-9-19
Published in Print: 2017-3-1

©2017 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/hsz-2016-0142
Keywords for this article
interaction; Pokemon; RARα; transcriptional activity

Articles in the same Issue

  1. Frontmatter
  2. Reviews
  3. Lysosomes in programmed cell death pathways: from initiators to amplifiers
  4. Mitochondrial carriers in inflammation induced by bacterial endotoxin and cytokines
  5. Recent insights into nitrite signaling processes in blood
  6. Research Articles/Short Communications
  7. Genes and Nucleic Acids
  8. Pokemon decreases the transcriptional activity of RARα in the absence of ligand
  9. Protein Structure and Function
  10. Comparison of enzymatic properties and small molecule inhibition of γ–glutamyltranspeptidases from pathogenic and commensal bacteria
  11. Structural and lipid-binding characterization of human annexin A13a reveals strong differences with its long A13b isoform
  12. Proteolysis
  13. A role for the metalloprotease invadolysin in insulin signaling and adipogenesis
  14. Mirolysin, a LysargiNase from Tannerella forsythia, proteolytically inactivates the human cathelicidin, LL-37
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