Startseite Kinin B1 receptor mediates memory impairment in the rat hippocampus
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Kinin B1 receptor mediates memory impairment in the rat hippocampus

  • Káris Ester Dong-Creste , Ticiana Baraldi-Tornisielo , Ariadiny Lima Caetano , Fernand Gobeil , Wagner Ricardo Montor , Tania Araujo Viel und Hudson Sousa Buck EMAIL logo
Veröffentlicht/Copyright: 15. Dezember 2015

Abstract

The bradykinin (BK) receptors B1R and B2R are involved in inflammatory responses and their activation can enhance tissue damage. The B2R is constitutively expressed and mediates the physiologic effects of BK, whereas B1R expression is induced after tissue damage. Recently, they have been involved with Alzheimer’s disease, ischemic stroke and traumatic brain injury (TBI). In this study, we investigated the role of bradykinin in short and long-term memory consolidation (STM and LTM). It was observed that bilateral injection of BK (300 pmol/μl) disrupted the STM consolidation but not LTM, both evaluated by inhibitory avoidance test. The STM disruption due to BK injection was blocked by the previous injection of the B1R antagonist des-Arg10-HOE140 but not by the B2R antagonist HOE140. Additionally, the injection of the B1 agonist desArg9-BK disrupted STM and LTM consolidation at doses close to physiological concentration of the peptide (2.3 and 37.5 pmol, respectively) which could be reached during tissue injury. The presence of B1R located on glial cells around the implanted guide cannula used for peptide injection was confirmed by immunofluorescence. These data imply in a possible participation of B1R in the STM impairment observed in TBI, neuroinflammation and neurodegeneration.


Corresponding author: Hudson Sousa Buck, Department of Physiological Sciences, Santa Casa de São Paulo School of Medical Science, R. Dr. Cesario Motta Junior, 61, 11° andar, São Paulo, SP 01221-020, Brazil; and Research Group on Neuropharmacology of Aging, Sao Paulo, Brazil, e-mail:

Acknowledgments

We thank Professor Tatiana Rosado Rosenstock for the assistance in the image acquisition. This work was supported by grants from FAP-Santa Casa, Fundação de Amparo a Pesquisa no Estado de Sao Paulo (FAPESP 2007/04800-0 and 2013/013656-1). H.S. Buck holds a Level 2 CNPQ fellowship (303283/2014-9). K.E. Dong-Creste and T. Baraldi-Tornisielo received fellowships from CAPES-PROSUP. Confocal images were obtained at the INFAR/UNIFESP Confocal and Flow Cytometry Facility, which is supported by FAPESP.

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Received: 2015-8-27
Accepted: 2015-12-9
Published Online: 2015-12-15
Published in Print: 2016-4-1

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