Abstract
The antiepileptic drug phenobarbital (PB) exerts hepatic effects related to cell proliferation and tumorigenesis which are closely linked to the Wnt/β-catenin signaling pathway. This pathway is, amongst others, regulated by calpain proteases. We now identified PB as an inhibitor of Wnt/β-catenin signaling in mouse hepatoma cells. Further analyses revealed that PB inhibits calpain activity, an effect which is at least in parts mediated by a transcriptional regulation of calpain mRNA levels and which is furthermore independent of the constitutive androstane receptor, the known mediator of most effects of PB in liver cells.
Acknowledgments
The authors acknowledge expert technical assistance by Silvia Vetter.
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©2016 by De Gruyter
Articles in the same Issue
- Frontmatter
- Reviews
- Ancestral protein reconstruction: techniques and applications
- Mapping the non-standardized biases of ribosome profiling
- Minireview
- The macromolecular crowding effect – from in vitro into the cell
- Research Articles/Short Communications
- Protein Structure and Function
- IsoQC (QPCTL) knock-out mice suggest differential substrate conversion by glutaminyl cyclase isoenzymes
- Molecular Medicine
- Correlated overexpression of metadherin and SND1 in glioma cells
- Cell Biology and Signaling
- Melanoma differentiation-associated gene 5 is involved in the induction of stress granules and autophagy by protonophore CCCP
- Suberoylanilide hydroxamic acid (SAHA) promotes the epithelial mesenchymal transition of triple negative breast cancer cells via HDAC8/FOXA1 signals
- Melanocytes are more responsive to IFN-γ and produce higher amounts of kynurenine than melanoma cells
- Phenobarbital inhibits calpain activity and expression in mouse hepatoma cells
Articles in the same Issue
- Frontmatter
- Reviews
- Ancestral protein reconstruction: techniques and applications
- Mapping the non-standardized biases of ribosome profiling
- Minireview
- The macromolecular crowding effect – from in vitro into the cell
- Research Articles/Short Communications
- Protein Structure and Function
- IsoQC (QPCTL) knock-out mice suggest differential substrate conversion by glutaminyl cyclase isoenzymes
- Molecular Medicine
- Correlated overexpression of metadherin and SND1 in glioma cells
- Cell Biology and Signaling
- Melanoma differentiation-associated gene 5 is involved in the induction of stress granules and autophagy by protonophore CCCP
- Suberoylanilide hydroxamic acid (SAHA) promotes the epithelial mesenchymal transition of triple negative breast cancer cells via HDAC8/FOXA1 signals
- Melanocytes are more responsive to IFN-γ and produce higher amounts of kynurenine than melanoma cells
- Phenobarbital inhibits calpain activity and expression in mouse hepatoma cells