A simple and highly sensitive method of measuring heme oxygenase activity
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Pham Hieu Liem
Abstract
Heme oxygenase (HO) is a rate-limiting step of heme degradation, which catalyzes the conversion of heme into biliverdin, iron, and CO. HO has been characterized in microorganisms, insects, plants, and mammals. Previously used assays of HO activity were complicated and had low sensitivity. We found that the use of an eel bilirubin-bound fluorescent protein, UnaG, can achieve a highly sensitive and simple assay of HO activity. Using several enzyme sources including human culture cells, homogenates of plant tissues, and recombinant yeast HO, data were successfully obtained. The present method can facilitate the examination of HO in various organisms.
Acknowledgments
The authors thank Dr. Atsushi Miyawaki for a kind gift of pcDNA3-UnaG. This study was supported in part by grants from the Ministry of Health, Labor and Welfare of Japan.
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©2015 by De Gruyter
Artikel in diesem Heft
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Artikel in diesem Heft
- Frontmatter
- Reviews
- Defects of corneocyte structural proteins and epidermal barrier in atopic dermatitis
- C-reactive protein and inflammation: conformational changes affect function
- Biogenesis of mitochondrial outer membrane proteins, problems and diseases
- Hepatitis C virus and autophagy
- Minireviews
- The glucocorticoid receptor in inflammatory processes: transrepression is not enough
- Red blood cells in Rett syndrome: oxidative stress, morphological changes and altered membrane organization
- Research Articles/Short Communications
- Membranes, Lipids, Glycobiology
- High β-glucosidase (GBA) activity not attributable to GBA1 and GBA2 in live normal and enzyme-deficient fibroblasts may emphasise the role of additional GBAs
- Cell Biology and Signaling
- Exogenous hydrogen sulfide exhibits anti-cancer effects though p38 MAPK signaling pathway in C6 glioma cells
- Novel Techniques
- Impact of food components during in vitro digestion of silver nanoparticles on cellular uptake and cytotoxicity in intestinal cells
- A simple and highly sensitive method of measuring heme oxygenase activity
