Central role of the Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) in sodium bioenergetics of Vibrio cholerae
Abstract
Vibrio cholerae is a Gram-negative bacterium that lives in brackish or sea water environments. Strains of V. cholerae carrying the pathogenicity islands infect the human gut and cause the fatal disease cholera. Vibrio cholerae maintains a Na+ gradient at its cytoplasmic membrane that drives substrate uptake, motility, and efflux of antibiotics. Here, we summarize the major Na+-dependent transport processes and describe the central role of the Na+-translocating NADH:quinone oxidoreductase (Na+-NQR), a primary Na+ pump, in maintaining a Na+-motive force. The Na+-NQR is a membrane protein complex with a mass of about 220 kDa that couples the exergonic oxidation of NADH to the transport of Na+ across the cytoplasmic membrane. We describe the molecular architecture of this respiratory complex and summarize the findings how electron transport might be coupled to Na+-translocation. Moreover, recent advances in the determination of the three-dimensional structure of this complex are reported.
Acknowledgments
We thank the staff at beamlines X06SA and X06DA at Swiss Light Source for excellent support. This work was supported by contract research ‘Methoden in den Lebenswissenschaften’ of the Baden-Württemberg Stiftung P-LS-Meth/4 (to J.S., and G.F.), and by the Deutsche Forschungsgemeinschaft grant FR 1321/3-1 (to J.S.) and grant FR 1488/3-2 (to G.F.).
References
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Articles in the same Issue
- Frontmatter
- Guest Editorial
- Highlight: Membrane transport on the move
- HIGHLIGHT: 9TH TRANSPORT COLLOQUIUM
- Small membrane proteins – elucidating the function of the needle in the haystack
- When two turn into one: evolution of membrane transporters from half modules
- Central role of the Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) in sodium bioenergetics of Vibrio cholerae
- Principles and mechanisms of CD95 activation
- Stitching proteins into membranes, not sew simple
- Cell-free expression of G-protein coupled receptors: new pipelines for challenging targets
- Voltage-dependent anion channels: the wizard of the mitochondrial outer membrane
- Structural characterization of a C-terminally truncated E5 oncoprotein from papillomavirus in lipid bilayers
- Minireview
- Genetic variation within transcriptional regulatory elements and its implications for human disease
- Research Articles/Short Communications
- Molecular Medicine
- Methotrexate-gelonin conjugate – an inhibitor of MCF-7 cells expressing the dihydrofolate receptor
Articles in the same Issue
- Frontmatter
- Guest Editorial
- Highlight: Membrane transport on the move
- HIGHLIGHT: 9TH TRANSPORT COLLOQUIUM
- Small membrane proteins – elucidating the function of the needle in the haystack
- When two turn into one: evolution of membrane transporters from half modules
- Central role of the Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) in sodium bioenergetics of Vibrio cholerae
- Principles and mechanisms of CD95 activation
- Stitching proteins into membranes, not sew simple
- Cell-free expression of G-protein coupled receptors: new pipelines for challenging targets
- Voltage-dependent anion channels: the wizard of the mitochondrial outer membrane
- Structural characterization of a C-terminally truncated E5 oncoprotein from papillomavirus in lipid bilayers
- Minireview
- Genetic variation within transcriptional regulatory elements and its implications for human disease
- Research Articles/Short Communications
- Molecular Medicine
- Methotrexate-gelonin conjugate – an inhibitor of MCF-7 cells expressing the dihydrofolate receptor