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Central role of the Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) in sodium bioenergetics of Vibrio cholerae

  • Julia Steuber , Petra Halang , Thomas Vorburger , Wojtek Steffen , Georg Vohl and Günter Fritz EMAIL logo
Published/Copyright: September 2, 2014

Abstract

Vibrio cholerae is a Gram-negative bacterium that lives in brackish or sea water environments. Strains of V. cholerae carrying the pathogenicity islands infect the human gut and cause the fatal disease cholera. Vibrio cholerae maintains a Na+ gradient at its cytoplasmic membrane that drives substrate uptake, motility, and efflux of antibiotics. Here, we summarize the major Na+-dependent transport processes and describe the central role of the Na+-translocating NADH:quinone oxidoreductase (Na+-NQR), a primary Na+ pump, in maintaining a Na+-motive force. The Na+-NQR is a membrane protein complex with a mass of about 220 kDa that couples the exergonic oxidation of NADH to the transport of Na+ across the cytoplasmic membrane. We describe the molecular architecture of this respiratory complex and summarize the findings how electron transport might be coupled to Na+-translocation. Moreover, recent advances in the determination of the three-dimensional structure of this complex are reported.


Corresponding author: Günter Fritz, Institute of Neuropathology, University of Freiburg, Breisacher Strasse 64, D-79106 Freiburg, Germany, e-mail:

Acknowledgments

We thank the staff at beamlines X06SA and X06DA at Swiss Light Source for excellent support. This work was supported by contract research ‘Methoden in den Lebenswissenschaften’ of the Baden-Württemberg Stiftung P-LS-Meth/4 (to J.S., and G.F.), and by the Deutsche Forschungsgemeinschaft grant FR 1321/3-1 (to J.S.) and grant FR 1488/3-2 (to G.F.).

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Received: 2014-6-1
Accepted: 2014-7-9
Published Online: 2014-9-2
Published in Print: 2014-12-1

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