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Lucimycin, an antifungal peptide from the therapeutic maggot of the common green bottle fly Lucilia sericata

  • Anne-Kathrin Pöppel , Aline Koch , Karl-Heinz Kogel , Heiko Vogel , Christian Kollewe , Jochen Wiesner and Andreas Vilcinskas EMAIL logo
Published/Copyright: March 12, 2014

Abstract

We report the identification, cloning, heterologous expression and functional characterization of a novel antifungal peptide named lucimycin from the common green bottle fly Lucilia sericata. The lucimycin cDNA was isolated from a library of genes induced during the innate immune response in L. sericata larvae, which are used as therapeutic maggots. The peptide comprises 77 amino acid residues with a molecular mass of 8.2 kDa and a pI of 6.6. It is predicted to contain a zinc-binding motif and to form a random coil, lacking β-sheets or other secondary structures. Lucimycin was active against fungi from the phyla Ascomycota, Basidiomycota and Zygomycota, in addition to the oomycete Phytophtora parasitica, but it was inactive against bacteria. A mutant version of lucimycin, lacking the four C-terminal amino acid residues, displayed 40-fold lower activity. The activity of lucimycin against a number of highly-destructive plant pathogens could be exploited to produce transgenic crops that are resistant against fungal diseases.


Corresponding author: Andreas Vilcinskas, Fraunhofer Institute for Molecular Biology and Applied Ecology, Department of Bioresources, Winchester Strasse, D-35394 Giessen, Germany; and Institute for Phytopathology and Applied Zoology, Justus-Liebig-University of Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany, e-mail:

Acknowledgments

This work was supported by the excellence initiative of the Hessian Ministry of Science, Higher Education and Art (HMWK) which encompasses a generous grant for the LOEWE research focus ‘Insect Biotechnology’ to A.V. and K.K. We thank Dr. Kerstin Voigt from the Jena Microbial Resource Collection, Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e.V., Hans-Knöll-Institut (HKI) for the antifungal assays and Christian René Röhrich for LC/MS analysis. The authors acknowledge Dr. Richard M. Twyman for editing the manuscript.

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Received: 2013-10-5
Accepted: 2014-3-11
Published Online: 2014-3-12
Published in Print: 2014-6-1

©2014 by Walter de Gruyter Berlin/Boston

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