Lucimycin, an antifungal peptide from the therapeutic maggot of the common green bottle fly Lucilia sericata
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Anne-Kathrin Pöppel
, Aline Koch , Karl-Heinz Kogel , Heiko Vogel , Christian Kollewe , Jochen Wiesner and Andreas Vilcinskas
Abstract
We report the identification, cloning, heterologous expression and functional characterization of a novel antifungal peptide named lucimycin from the common green bottle fly Lucilia sericata. The lucimycin cDNA was isolated from a library of genes induced during the innate immune response in L. sericata larvae, which are used as therapeutic maggots. The peptide comprises 77 amino acid residues with a molecular mass of 8.2 kDa and a pI of 6.6. It is predicted to contain a zinc-binding motif and to form a random coil, lacking β-sheets or other secondary structures. Lucimycin was active against fungi from the phyla Ascomycota, Basidiomycota and Zygomycota, in addition to the oomycete Phytophtora parasitica, but it was inactive against bacteria. A mutant version of lucimycin, lacking the four C-terminal amino acid residues, displayed 40-fold lower activity. The activity of lucimycin against a number of highly-destructive plant pathogens could be exploited to produce transgenic crops that are resistant against fungal diseases.
Acknowledgments
This work was supported by the excellence initiative of the Hessian Ministry of Science, Higher Education and Art (HMWK) which encompasses a generous grant for the LOEWE research focus ‘Insect Biotechnology’ to A.V. and K.K. We thank Dr. Kerstin Voigt from the Jena Microbial Resource Collection, Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e.V., Hans-Knöll-Institut (HKI) for the antifungal assays and Christian René Röhrich for LC/MS analysis. The authors acknowledge Dr. Richard M. Twyman for editing the manuscript.
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©2014 by Walter de Gruyter Berlin/Boston
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Articles in the same Issue
- Frontmatter
- Reviews
- The top skin-associated genes: a comparative analysis of human and mouse skin transcriptomes
- Human dyskerin: beyond telomeres
- Assembly and function of small RNA – Argonaute protein complexes
- Minireview
- The globin superfamily: functions in nitric oxide formation and decay
- Research Articles/Short Communications
- Protein Structure and Function
- N-homocysteinylation of apolipoprotein A-I impairs the protein’s antioxidant ability but not its cholesterol efflux capacity
- Lucimycin, an antifungal peptide from the therapeutic maggot of the common green bottle fly Lucilia sericata
- A lysine-methionine exchange in a coronavirus surface protein transforms a retention motif into an endocytosis signal
- Cell Biology and Signaling
- Upregulation of the thioredoxin-dependent redox system during differentiation of 3T3-L1 cells to adipocytes
- Novel Techniques
- Shortening distance of forward and reverse primers for nucleic acid isothermal amplification