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Eternity and functionality – rational access to physiologically relevant cell lines

  • Christoph Lipps

    Christoph Lipps studied Biosystems Engineering at the Otto-von-Guericke University in Magdeburg, Germany (2005–2010). In 2010 he started his PhD thesis at the Helmholtz Centre for Infection Research in the group ‘Model Systems for Infection and Immunity’. He is working on the generation of hepatocyte cell lines with preserved hepatocyte specific phenotype in vitro and in vivo.

         , Tobias May

    Tobias May studied Biochemistry at the Martin Luther University in Halle-Wittenberg (1996–2001). Afterwards he did his PhD thesis at the former Gesellschaft für Biotechnologische Forschung GmbH on the development of a transcriptionally regulated system for conditional immortalization (2001–2005). Following this his work at the Helmholtz Centre for Infection Research focused on the development of novel technologies allowing an in vitro cell expansion of primary cell (2005–2009). In 2009 he co-founded the InSCREENeX GmbH, a spin-off from the HZI, which commercializes the physiologically relevant cell systems established by the different immortalization technologies.

         , Hansjörg Hauser

    Hansjörg Hauser graduated in Biology (Dr. rer. nat.) at the University of Konstanz, Germany. He completed his postdoctoral training at the Max-Planck-Institute for Molecular Genetics, Berlin, and at the German Cancer Research Center, Heidelberg and became staff scientist of the Helmholtz Centre for Infection Research, HZI (formerly Gesellschaft für Biotechnologische Forschung, GBF) where he assumed leading positions as head of Division Molecular Biotechnology and head of Department Gene Regulation and Differentiation. His expertise is molecular biology. He is active in translational research concerning gene expression in biotechnology and gene/cell therapies.

     EMAIL logo     und Dagmar Wirth

    Dagmar Wirth studied chemistry and obtained her PhD in Molecular Biotechnology from the University of Braunschweig. She did her PostDoc at the Gesellschaft für Biotechnologische Forschung and the Medical University of Hannover. Since 2007 she is head of the research group ‘Model Systems for Infection and Immunity’ at the Helmholtz Centre for Infection Research. Her research focusses on the development of cell and mouse models for various questions in virology and immune biology. Special emphasis is laid on the development of novel cell based models by (conditional) immortalization which are employed in vitro as well as in vivo.
Veröffentlicht/Copyright: 16. Juli 2013
Biological Chemistry
Aus der Zeitschrift Biological Chemistry Band 394 Heft 12

Abstract

In the first 50 years of cell culture, the development of new cell lines was mainly based on trial and error. Due to the understanding of the molecular networks of aging, senescence, proliferation, and adaption by mutation, the generation of new cell lines with physiologic properties has become more systematic. This endeavor has been supported by the availability of new technological achievements and increasing knowledge about the biology of cell differentiation and cell-cell communication. Here, we review some promising developments that are contributing toward this goal. These include molecular tools frequently used for the immortalization process. In addition to these broadly acting immortalization regimens, we focus on the developments of cell type-specific immortalization and on the methodologies of how to control the growth of newly established cell lines.

Keywords: cell type-specific immortalization; growth-controlled cell lines; immortalization; immortalizing genes; molecular mechanisms of immortalization; physiologically relevant cell lines
Corresponding author: Hansjörg Hauser, Department of Gene Regulation and Differentiation, Helmholtz Centre for Infection Research, Inhoffenstr. 7, D-38124 Braunschweig, Germany, e-mail:

About the authors

Christoph Lipps

Christoph Lipps studied Biosystems Engineering at the Otto-von-Guericke University in Magdeburg, Germany (2005–2010). In 2010 he started his PhD thesis at the Helmholtz Centre for Infection Research in the group ‘Model Systems for Infection and Immunity’. He is working on the generation of hepatocyte cell lines with preserved hepatocyte specific phenotype in vitro and in vivo.

Tobias May

Tobias May studied Biochemistry at the Martin Luther University in Halle-Wittenberg (1996–2001). Afterwards he did his PhD thesis at the former Gesellschaft für Biotechnologische Forschung GmbH on the development of a transcriptionally regulated system for conditional immortalization (2001–2005). Following this his work at the Helmholtz Centre for Infection Research focused on the development of novel technologies allowing an in vitro cell expansion of primary cell (2005–2009). In 2009 he co-founded the InSCREENeX GmbH, a spin-off from the HZI, which commercializes the physiologically relevant cell systems established by the different immortalization technologies.

Hansjörg Hauser

Hansjörg Hauser graduated in Biology (Dr. rer. nat.) at the University of Konstanz, Germany. He completed his postdoctoral training at the Max-Planck-Institute for Molecular Genetics, Berlin, and at the German Cancer Research Center, Heidelberg and became staff scientist of the Helmholtz Centre for Infection Research, HZI (formerly Gesellschaft für Biotechnologische Forschung, GBF) where he assumed leading positions as head of Division Molecular Biotechnology and head of Department Gene Regulation and Differentiation. His expertise is molecular biology. He is active in translational research concerning gene expression in biotechnology and gene/cell therapies.

Dagmar Wirth

Dagmar Wirth studied chemistry and obtained her PhD in Molecular Biotechnology from the University of Braunschweig. She did her PostDoc at the Gesellschaft für Biotechnologische Forschung and the Medical University of Hannover. Since 2007 she is head of the research group ‘Model Systems for Infection and Immunity’ at the Helmholtz Centre for Infection Research. Her research focusses on the development of cell and mouse models for various questions in virology and immune biology. Special emphasis is laid on the development of novel cell based models by (conditional) immortalization which are employed in vitro as well as in vivo.

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Received: 2013-4-9
Accepted: 2013-7-11
Published Online: 2013-07-16
Published in Print: 2013-12-01

©2013 by Walter de Gruyter Berlin Boston

Artikel in diesem Heft

  1. Masthead
  2. Masthead
  3. Guest Editorial
  4. Highlight: perspectives in molecular neurobiology
  5. HIGHLIGHT: PERSPECTIVES IN MOLECULAR NEUROBIOLOGY
  6. Role of the peroxisome proliferator-activated receptors (PPAR)-α, β/δ and γ triad in regulation of reactive oxygen species signaling in brain
  7. Molecular triggers of neuroinflammation in mouse models of demyelinating diseases
  8. Sox appeal – Sox10 attracts epigenetic and transcriptional regulators in myelinating glia
  9. MeCP2 phosphorylation in the brain: from transcription to behavior
  10. The nuclear receptor peroxisome proliferator-activated receptor-γ promotes oligodendrocyte differentiation through mechanisms involving mitochondria and oscillatory Ca2+ waves
  11. G-protein-coupled designer receptors – new chemical-genetic tools for signal transduction research
  12. Prolonged cultivation of hippocampal neural precursor cells shifts their differentiation potential and selects for aneuploid cells
  13. Reviews
  14. Eternity and functionality – rational access to physiologically relevant cell lines
  15. Structural and functional insights into the Spir/formin actin nucleator complex
https://doi.org/10.1515/hsz-2013-0158
Schlagwörter für diesen Artikel
cell type-specific immortalization; growth-controlled cell lines; immortalization; immortalizing genes; molecular mechanisms of immortalization; physiologically relevant cell lines

Artikel in diesem Heft

  1. Masthead
  2. Masthead
  3. Guest Editorial
  4. Highlight: perspectives in molecular neurobiology
  5. HIGHLIGHT: PERSPECTIVES IN MOLECULAR NEUROBIOLOGY
  6. Role of the peroxisome proliferator-activated receptors (PPAR)-α, β/δ and γ triad in regulation of reactive oxygen species signaling in brain
  7. Molecular triggers of neuroinflammation in mouse models of demyelinating diseases
  8. Sox appeal – Sox10 attracts epigenetic and transcriptional regulators in myelinating glia
  9. MeCP2 phosphorylation in the brain: from transcription to behavior
  10. The nuclear receptor peroxisome proliferator-activated receptor-γ promotes oligodendrocyte differentiation through mechanisms involving mitochondria and oscillatory Ca2+ waves
  11. G-protein-coupled designer receptors – new chemical-genetic tools for signal transduction research
  12. Prolonged cultivation of hippocampal neural precursor cells shifts their differentiation potential and selects for aneuploid cells
  13. Reviews
  14. Eternity and functionality – rational access to physiologically relevant cell lines
  15. Structural and functional insights into the Spir/formin actin nucleator complex
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