Association of serum NF-κB levels with peripheral neuropathy in type 2 diabetes mellitus patients: a pilot study

Nibedita Priyadarsini; Madumathy Ramachandran; Kishore K. Behera; Sheetal Kiran; Sujata Devi

doi:10.1515/hmbci-2022-0105

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Article

Association of serum NF-κB levels with peripheral neuropathy in type 2 diabetes mellitus patients: a pilot study

Nibedita Priyadarsini , Madumathy Ramachandran , Kishore K. Behera , Sheetal Kiran and Sujata Devi

Published/Copyright: March 21, 2024

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From the journal Hormone Molecular Biology and Clinical Investigation Volume 45 Issue 1

Abstract

Objectives

Hyperglycaemia-induced inflammation plays a vital role in the development of diabetic peripheral neuropathy (DPN). Recent evidences had reported the involvement of the transcription factor nuclear factor kappa-light-chain-enhancer of activated B cell (NF-κB) in diabetic experimental models. So, this pilot study aimed to evaluate serum NF-κB levels in DPN patients.

Methods

We recruited 50 T2DM patients, of which 25 were T2DM with neuropathy and 25 were T2DM without neuropathy. In all the participants peripheral neuropathy was diagnosed based on Total neuropathy score (TNS). Serum NF-κB levels were measured by ELISA.

Results

We observed that the serum NF-κB levels were higher in DPN patients in comparison to T2DM patients without neuropathy. On spearman correlation, a positive correlation was found between serum NF-κB levels and TNS in the DPN group (r=0.741, p<0.001). The regression model shows the TNS to be an independent determinant of serum NF-κB levels after adjustment for potential confounders like age, duration of diabetes, and HbA1C (B=81.34; p<0.001).

Conclusions

NF-κB activation plays a key role in promoting inflammation which is associated with the progression of DPN. In this respect, the study of NF-κB levels in serum may be an additional diagnostic marker for DPN.

Keywords: diabetic peripheral neuropathy; hyperglycemia; inflammation; NF-κB; type-2 diabetes mellitus

Corresponding author: Dr. Nibedita Priyadarsini, Department of Physiology, All India Institute of Medical Sciences, Bhubaneswar, Odisha 751019, India, Phone: +919938569067, E-mail: drnibeditap@gmail.com

Funding source: All India Institute of Medical Sciences, Bhubaneswar

Award Identifier / Grant number: IMF/37/2018

Acknowledgments

We thank our technical staff for extending their support throughout the project. We sincerely express our gratitude to All India Institute of Medical Sciences, Bhubaneswar for funding our project.

Research ethics: The approval was obtained from Institute Ethics Committee for human studies with reference no. T/IM-F/18-19/16 before the commencement of the study.
References.
Informed consent: Informed consent was obtained from all individuals included in this study.
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: Authors state no conflict of interest.
Research funding: This study was funded by All India Institute of Medical Sciences, Bhubaneswar funding agency with project code No. IMF/37/2018.
Data availability: The raw data can be obtained on request from the correspondoing author.

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Received: 2022-11-20

Accepted: 2024-03-06

Published Online: 2024-03-21

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Articles in the same Issue

https://doi.org/10.1515/hmbci-2022-0105

Keywords for this article

diabetic peripheral neuropathy; hyperglycemia; inflammation; NF-κB; type-2 diabetes mellitus