Comparative study of radioprotective effects of endurance training in irradiation-induced nephropathy of rat model
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Mohammad Parastesh
,
Shiva Molavi
and
Samira Moghadasi
Abstract
Objectives
Considering the antioxidant properties of endurance training, this study aimed to investigate the effects of endurance training on serum levels of oxidative stress and structural changes in the kidney tissue of rats exposed to X-ray irradiation.
Methods
In this experimental study, 24 rats weighing 220±20 g were randomly divided into four groups (healthy control, healthy with moderate-intensity continuous training, X-ray control, and X-ray with moderate-intensity continuous training). The two groups of rats were irradiated with 4 Gy X-rays. The two training groups also performed moderate-intensity continuous training for 10 weeks. Twenty-four hour after the last training session, the blood serum of rats was collected and kidney tissue was isolated for stereological studies.
Results
In this study, X-ray irradiation of the whole body of rats caused a significant increase in kidney volume, cortex volume, interstitial tissue volume, glomerular volume, and serum level of MDA (p≤0.05), but the medulla volume, volume of proximal tubules (total volume, volume of epithelium, and lumen), volume of distal tubules (total volume, volume of epithelium, and lumen), and the length of the proximal and distal tubules had no effect. In addition, TAC and SOD levels were significantly decreased in the radiation control group. Furthermore, performing endurance training in X-ray-irradiated rats significantly reduced kidney volume, cortex volume, glomerular volume, and serum MDA level (p≤0.05).
Conclusions
Moderate-intensity continuous training can improve the rate of destruction of kidney tissue in rats exposed to X-rays by reducing oxidative stress and subsequently increasing antioxidant capacity.
Acknowledgments
The authors are thankful for subjects who participated in the study.
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Research ethics: The study was approved by the Ethics Committee of Arak University of Medical Sciences (Ethics Code: IR.Arakmu.rec.1400.205).
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Informed consent: For this study, informed consent statements were signed by all the subjects. Author contributions All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: All authors of this paper declare that; they don’t have any conflicts of interest related to the submission.
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Research funding: This study received no funding.
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Data availability: Data are available on reasonable request. Other supplementary data are available on reasonable request.
References
1. Davies, B, Manning-Stanley, A, Hughes, V. Gonad shield placement accuracy in pelvic radiographs for male patients: a prospective phantom study and survey of third year undergraduate diagnostic radiography students. Radiography 2022;28:366–71. https://doi.org/10.1016/j.radi.2022.02.001.Search in Google Scholar PubMed
2. Delaney, GP, Barton, MB. Evidence-based estimates of the demand for radiotherapy. Clin Oncol 2015;27:70–6. https://doi.org/10.1016/j.clon.2014.10.005.Search in Google Scholar PubMed
3. Joubert, A, Vogin, G, Granzotto, A, Devic, C, Viau, M, Thomas, C, et al.. Radiation biology: major advances and perspectives for radiotherapy; biologie des radiations: avancees majeures et perspectives pour la radiotherapie. Cancer Radiother 2011;15:348–54. https://doi.org/10.1016/j.canrad.2011.05.001.Search in Google Scholar PubMed
4. Jung, AY, Cai, X, Thoene, K, Obi, N, Jaskulski, S, Behrens, S, et al.. Antioxidant supplementation and breast cancer prognosis in postmenopausal women undergoing chemotherapy and radiation therapy. Am J Clin Nutr 2019;109:69–78. https://doi.org/10.1093/ajcn/nqy223.Search in Google Scholar PubMed
5. Palma, HE, Wolkmer, P, Gallio, M, Corrêa, MM, Schmatz, R, Thomé, GR, et al.. Oxidative stress parameters in blood, liver, and kidney of diabetic rats treated with curcumin and/or insulin. Mol Cell Biochem 2014;386:199–210. https://doi.org/10.1007/s11010-013-1858-5.Search in Google Scholar PubMed
6. Ravarotto, V, Simioni, F, Pagnin, E, Davis, PA, Calò, LA. Oxidative stress–chronic kidney disease–cardiovascular disease: a vicious circle. Life Sci 2018;210:125–31. https://doi.org/10.1016/j.lfs.2018.08.067.Search in Google Scholar PubMed
7. Liu, Y-Q, Wang, X-L, He, D-H, Cheng, Y-X. Protection against chemotherapy-and radiotherapy-induced side effects: a review based on the mechanisms and therapeutic opportunities of phytochemicals. Phytomedicine 2021;80:153402. https://doi.org/10.1016/j.phymed.2020.153402.Search in Google Scholar PubMed
8. Borek, C. Antioxidants and radiation therapy. J Nutr 2004;134:3207S–9S. https://doi.org/10.1093/jn/134.11.3207s.Search in Google Scholar PubMed
9. Abdel-Magied, N, Elkady, AA. Possible curative role of curcumin and silymarin against nephrotoxicity induced by gamma-rays in rats. Exp Mol Pathol 2019;111:104299. https://doi.org/10.1016/j.yexmp.2019.104299.Search in Google Scholar PubMed
10. de Sousa, CV, Sales, MM, Rosa, TS, Lewis, JE, de Andrade, RV, Simões, HG. The antioxidant effect of exercise: a systematic review and meta-analysis. Sports Med 2017;47:277–93. https://doi.org/10.1007/s40279-016-0566-1.Search in Google Scholar PubMed
11. Formigari, GP, Dátilo, MN, Vareda, B, Bonfante, ILP, Cavaglieri, CR, Lopes de Faria, JM, et al.. Renal protection induced by physical exercise may be mediated by the irisin/AMPK axis in diabetic nephropathy. Sci Rep 2022;12:9062. https://doi.org/10.1038/s41598-022-13054-y.Search in Google Scholar PubMed PubMed Central
12. Zimmer, P, Schmidt, ME, Prentzell, MT, Berdel, B, Wiskemann, J, Kellner, KH, et al.. Resistance exercise reduces kynurenine pathway metabolites in breast cancer patients undergoing radiotherapy. Front Oncol 2019;9:962. https://doi.org/10.3389/fonc.2019.00962.Search in Google Scholar PubMed PubMed Central
13. Lynskey, GE III, Powell, DK, Dixon, RG, Silberzweig, JE. Radiation protection in interventional radiology: survey results of attitudes and use. J Vasc Intervent Radiol 2013;24:1547–51.e3. https://doi.org/10.1016/j.jvir.2013.05.039.Search in Google Scholar PubMed
14. Bayat, M, Alaee, M, Akbari, A, Sadegh, M, Latifi, SA, Parastesh, M, et al.. A comparative study of the antidiabetic effect of two training protocols in streptozotocin-nicotinamide diabetic rats. Horm Mol Biol Clin Invest 2020;41:20190046. https://doi.org/10.1515/hmbci-2019-0046.Search in Google Scholar PubMed
15. Mandarim-de-Lacerda, CA. Stereological tools in biomedical research. An Acad Bras Ciênc 2003;75:469–86. https://doi.org/10.1590/s0001-37652003000400006.Search in Google Scholar PubMed
16. Reed, M, Howard, C, De Yanés, GS. One-stop stereology: the estimation of 3D parameters using isotropic rulers. J Microsc 2010;239:54–65. https://doi.org/10.1111/j.1365-2818.2009.03356.x.Search in Google Scholar PubMed
17. Zeeb, H, Merzenich, H, Blettner, M, Wollschläger, D. Radiation epidemiology. In: Ahrens, W., Pigeot, I. editors. Handbook of epidemiology. New York, NY: Springer; 2023:1–39 pp. https://doi.org/10.1007/978-1-4614-6625-3_68-1.Search in Google Scholar
18. Kala, J. Radiation-induced kidney injury. J Onco-Nephrol 2019;3:160–7. https://doi.org/10.1177/2399369319865271.Search in Google Scholar
19. Ezz, MK, Ibrahim, NK, Said, MM, Farrag, MA. The beneficial radioprotective effect of tomato seed oil against gamma radiation–induced damage in male rats. J Diet Suppl 2018;15:923–38. https://doi.org/10.1080/19390211.2017.1406427.Search in Google Scholar PubMed
20. Clemente, SM, Martínez-Costa, OH, Monsalve, M, Samhan-Arias, AK. Targeting lipid peroxidation for cancer treatment. Molecules 2020;25:5144. https://doi.org/10.3390/molecules25215144.Search in Google Scholar PubMed PubMed Central
21. Özyurt, H, Çevik, Ö, Özgen, Z, Özden, A, Çadırcı, S, Elmas, M, et al.. Quercetin protects radiation-induced DNA damage and apoptosis in kidney and bladder tissues of rats. Free Radic Res 2014;48:1247–55. https://doi.org/10.3109/10715762.2014.945925.Search in Google Scholar PubMed
22. da Silva Marcelino, MC, Magalhães, WV, Fonseca, FLA, Nucci, RAB, Maifrino, LBM. Effects of resistance training on kidney morphology of aged ovariectomized rats. Acta Histochem 2020;122:151613. https://doi.org/10.1016/j.acthis.2020.151613.Search in Google Scholar PubMed
23. Amaral, LSB, Souza, CS, Lima, HN, Soares, TJ. Influence of exercise training on diabetic kidney disease: a brief physiological approach. Exp Biol Med 2020;245:1142–54. https://doi.org/10.1177/1535370220928986.Search in Google Scholar PubMed PubMed Central
24. Ruangthai, R, Phoemsapthawee, J. Combined exercise training improves blood pressure and antioxidant capacity in elderly individuals with hypertension. J Exerc Sci Fitness. 2019;17:67–76, https://doi.org/10.1016/j.jesf.2019.03.001.Search in Google Scholar PubMed PubMed Central
25. Parastesh, M, Yousefvand, Z, Moghadasi, S. Comparison of the effect of moderate-intensity interval training (MICT) and high-intensity interval training (HIIT) on testicular structure, serum level of malondialdehyde and total antioxidant capacity of male diabetic rats. Daneshvar Med 2020;27:27–40.Search in Google Scholar
26. Ortega, JAF, de Paz Fernández, JA. Effects of a combined strength and high-intensity aerobic exercise program in breast cancer survivors: a pilot study. Apunts Med Esport 2016;51:3–12. https://doi.org/10.1016/j.apunts.2015.10.003.Search in Google Scholar
27. Liu, JF, Chang, WY, Chan, KH, Tsai, WY, Lin, CL, Hsu, MC. Blood lipid peroxides and muscle damage increased following intensive resistance training of female weightlifters. Ann N Y Acad Sci 2005;1042:255–61. https://doi.org/10.1196/annals.1338.029.Search in Google Scholar PubMed
28. Kelkar, G, Subhadra, K, Chengappa, RK. Effect of antioxidant supplementation on hematological parameters, oxidative stress and performance of Indian athletes. J Hum Ecol 2008;24:209–13. https://doi.org/10.1080/09709274.2008.11906116.Search in Google Scholar
29. Powers, SK, Jackson, MJ. Exercise-induced oxidative stress: cellular mechanisms and impact on muscle force production. Physiol Rev 2008;88:1243–76. https://doi.org/10.1152/physrev.00031.2007.Search in Google Scholar PubMed PubMed Central
30. Vassalle, C, Pingitore, A, De Giuseppe, R, Vigna, L, Bamonti, F. Biomarkers part II: biomarkers to estimate bioefficacy of dietary/supplemental antioxidants in sport. In: Lamprecht, M, editor. Antioxidants in sport nutrition. Boca Raton, FL: CRC Press/Taylor & Francis; 2015:18 p.10.1201/b17442-17Search in Google Scholar
31. Gomez-Cabrera, M-C, Domenech, E, Viña, J. Moderate exercise is an antioxidant: upregulation of antioxidant genes by training. Free Radical Biol Med 2008;44:126–31. https://doi.org/10.1016/j.freeradbiomed.2007.02.001.Search in Google Scholar PubMed
32. Radak, Z, Chung, HY, Koltai, E, Taylor, AW, Goto, S. Exercise, oxidative stress and hormesis. Ageing Res Rev 2008;7:34–42. https://doi.org/10.1016/j.arr.2007.04.004.Search in Google Scholar PubMed
33. Grantham, JJ, Torres, VE. The importance of total kidney volume in evaluating progression of polycystic kidney disease. Nat Rev Nephrol 2016;12:667–77. https://doi.org/10.1038/nrneph.2016.135.Search in Google Scholar PubMed PubMed Central
34. Gailiūnienė, A, Stasiulis, A, Michailovienė, J. The effect of submaximal exercise on blood creatinine, urea, total protein and uric acid levels of trained and untrained subjects. Ugdymas Kūno kultūra Sportas 2007; 3:5–10.10.33607/bjshs.v3i66.542Search in Google Scholar
35. Bhat, S, Balakrishnan, G. Chapter 30 - Role of exercise in the prevention and treatment of metabolic syndrome. In: Mukhopadhyay S, mondal S, editors. Metabolic syndrome. Arak: Academic Press; 2024:367–81 pp.10.1016/B978-0-323-85732-1.00012-8Search in Google Scholar
© 2024 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Original Articles
- The nongenomic neuroprotective effects of estrogen, E2-BSA, and G1 following traumatic brain injury: PI3K/Akt and histopathological study
- Comparative study of radioprotective effects of endurance training in irradiation-induced nephropathy of rat model
- Association of serum NF-κB levels with peripheral neuropathy in type 2 diabetes mellitus patients: a pilot study
- Case Report
- Mature cystic ovarian teratoma with squamous cell carcinoma transformation: a case report and literature review
Articles in the same Issue
- Frontmatter
- Original Articles
- The nongenomic neuroprotective effects of estrogen, E2-BSA, and G1 following traumatic brain injury: PI3K/Akt and histopathological study
- Comparative study of radioprotective effects of endurance training in irradiation-induced nephropathy of rat model
- Association of serum NF-κB levels with peripheral neuropathy in type 2 diabetes mellitus patients: a pilot study
- Case Report
- Mature cystic ovarian teratoma with squamous cell carcinoma transformation: a case report and literature review
