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The effects of estradiol and testosterone on renal tissues oxidative after central injection of angiotensin II in female doca – salt treated rats

  • Marzieh Kafami EMAIL logo , Mahmoud Hosseini , Saeed Niazmand , Esmaeil Farrokhi , Mosa Al-Reza Hajzadeh and Samad Nazemi
Published/Copyright: November 6, 2018
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Hormone Molecular Biology and Clinical Investigation
From the journal Hormone Molecular Biology and Clinical Investigation Volume 37 Issue 3

Abstract

Background

Although numerous studies have proven that estrogen (Est) has a protective effect on the development of hypertension, more research needs to be done to show its detailed mechanism in a variety of hypertension. The important role of active oxygen species in blood pressure is well defined. We examined whether or not sex hormones change the growth of reactive oxygen species (ROS) ‎in kidneys after central microinjection of angiotensin II (Ang II).‎

Materials and methods

Female Wistar rats, 8 weeks old (200 ± 10 g) were used in this study. The animal groups were (1) Sham, (2) Ovariectomy (OVX), (3) Sham-Hypertension (Sham-Hyper), (4) OVX-Hypertension (OVX-Hyper), (5) Sham-Hyper-Est, (6) OVX-Hyper-Est‎;‎ (7) Sham-Hyper-Testosterone (Tst) and (8) OVX-Hyper-Tst. Solutions of 1% NaCl and 0.1 KCl ‎were used and desoxycorticostrone (doca-salt) was injected (45 mg/kg) 3 times a week in Hypertension groups. Estradiol and Tst (2 mg/kg and ‎5 mg/kg‎; daily; subcutaneously) for 4 weeks. Ang II (50 μM, 5 μL) was microinjected by intracerebroventricular ( i.c.v.) infusion and malondialdehyde (MDA) and thiol in the kidneys were measured.

Results

MDA in the kidneys was increased by Ang II and doca-salt treatments. Both estradiol and Tst decreased the kidney’s MDA. The level of thiol was higher in Hyper ‎groups and reversed after treatment with estradiol and Tst.

Conclusions

Our findings suggest that central effect of Ang II on blood pressure and kidney ‎disease is accompanied with increased levels of oxidative stress in the kidneys. Indeed sex hormones change the ROS level in the kidneys after central ‎microinjection of Ang II.‎‎

Keywords: angiotensin II; doca-salt; estradiol; oxidative stress; testosterone

Author Statement

  1. Research funding: Authors state no funding involved.

  2. Conflict of interest: Authors state no conflict of interest.

  3. Informed consent: Not applicable.

  4. Ethical approval: Animal procedures were carried out considering the Guide for the Care and Use of Laboratory Animals and were approved by the Committee on Animal Research of Mashhad University of Medical Sciences. The document number was 88425.

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Received: 2018-06-13
Accepted: 2018-09-11
Published Online: 2018-11-06

©2019 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Original Articles
  2. The effects of estradiol and testosterone on renal tissues oxidative after central injection of angiotensin II in female doca – salt treated rats
  3. Correlates of resistin and retinol-binding protein 4 in metabolic syndrome patients with and without prediabetes
  4. Correlates of zinc finger BED domain-containing protein 3 and ghrelin in metabolic syndrome patients with and without prediabetes
  5. Copper and zinc in stage I multiple myeloma: relation with ceruloplasmin, lipid peroxidation, and superoxide dismutase activity
  6. Short Communication
  7. Ovarian reserve in adolescent girls born prematurely
https://doi.org/10.1515/hmbci-2018-0044
Keywords for this article
angiotensin II; doca-salt; estradiol; oxidative stress; testosterone

Articles in the same Issue

  1. Original Articles
  2. The effects of estradiol and testosterone on renal tissues oxidative after central injection of angiotensin II in female doca – salt treated rats
  3. Correlates of resistin and retinol-binding protein 4 in metabolic syndrome patients with and without prediabetes
  4. Correlates of zinc finger BED domain-containing protein 3 and ghrelin in metabolic syndrome patients with and without prediabetes
  5. Copper and zinc in stage I multiple myeloma: relation with ceruloplasmin, lipid peroxidation, and superoxide dismutase activity
  6. Short Communication
  7. Ovarian reserve in adolescent girls born prematurely
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