Melatonin has a protective effect against lipid peroxidation in the bone tissue of diabetic rats subjected to acute swimming exercise
Abstract
Aim
The present study aimed to examine the effects of melatonin supplementation on lipid peroxidation in the bone tissue of diabetic rats subjected to acute swimming exercise.
Methods
The study was conducted on 80 Sprague-Dawley type adult male rats which were equally allocated to eight groups: group 1, general control; group 2, melatonin-supplemented control; group 3, melatonin-supplemented diabetic control; group 4, swimming control; group 5, melatonin-supplemented swimming; group 6, melatonin-supplemented diabetic swimming; group 7, diabetic swimming; group 8, diabetic control. In order to induce diabetes, the animals were subcutaneously injected with 40 mg/kg streptozotocin (STZ). The animals were supplemented with 3 mg/kg/day melatonin intraperitoneally (IP) for 4 weeks. At the end of the study, the animals were decapitated to collect bone tissue samples which were examined to find out the malondialdehyde (MDA) (nmol/g/protein) and glutathione (GSH) (mg/dL/g protein) levels.
Results
The highest MDA values in the bone tissue were found in groups 7 and 8. MDA levels in the bone tissue in groups 3 and 6 were lower than the levels in groups 7 and 8, but higher than those in all other groups. Groups 3, 5 and 6 had the highest bone tissue GSH values. On the other hand, the lowest GSH level was established in groups 7 and 8.
Conclusion
The results of the present study indicated that the cell damage caused by acute swimming exercise and diabetes in the bone tissue could be prevented by melatonin supplementation.
Funding source: Selcuk University
Award Identifier / Grant number: SUBAPK; project no. 08401082
Funding statement: This study was supported by the Scientific Research Projects Coordinatorship of Selcuk University (SUBAPK; project no. 08401082).
Author Statement
Conflict of interest: Authors declare no conflict of interest.
Informed consent: Informed consent is not applicable.
Ethical approval: The study protocol was approved by the Ethics Committee of Selcuk University Physical Education and Sport High School.
References
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©2018 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Original Articles
- Management of women with PCOS using myo-inositol and folic acid. New clinical data and review of the literature
- Melatonin has a protective effect against lipid peroxidation in the bone tissue of diabetic rats subjected to acute swimming exercise
- Short Communication
- Basic pituitary-ovarian hormones and ultrasound parameters of uterine appendages in full-term and preterm neonate girls
- Case Report
- A positive urine pregnancy test (UPT) with adnexal mass; ectopic pregnancy is not the ultimate diagnosis
Articles in the same Issue
- Original Articles
- Management of women with PCOS using myo-inositol and folic acid. New clinical data and review of the literature
- Melatonin has a protective effect against lipid peroxidation in the bone tissue of diabetic rats subjected to acute swimming exercise
- Short Communication
- Basic pituitary-ovarian hormones and ultrasound parameters of uterine appendages in full-term and preterm neonate girls
- Case Report
- A positive urine pregnancy test (UPT) with adnexal mass; ectopic pregnancy is not the ultimate diagnosis