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Optimization of Burkholderia sp. ZYB002 lipase production for pitch control in thermomechanical pulping (TMP) processes
-
Zhengyu Shu
,
Jiguang Wu
Veröffentlicht/Copyright:
26. September 2011
Abstract
Production and process optimization of a thermostable lipase from Burkholderia sp. ZYB002 and the application potential of this lipase for pitch control of thermomechanical pulp (TMP) has been investigated. The optimized physical and nutritional factors of Burkholderia sp. ZYB002 were found to be (all units in%, w v-1): 0.5 peptone, 0.025 urea, 0.5 glycerol, 0.1 NaHCO3, 0.2 K2HPO4, 1.5 Tween 80, 1.1 olive oil, four inoculum volume, and initial pH 8.5. The lipase production process was carried out at 30°C at 250 rpm for 36 h in 250 ml Erlenmeyer flasks containing 35 ml fermentation medium and five glass beads. Under the optimized conditions, lipase activity reached 216.5 U ml-1. In a TMP of Pinus massoniana, 39% of triglycerides were hydrolyzed by the lipase under optimized conditions [pulp concentration 6% (w v-1), lipase dose 20 U g-1 based on dry TMP, pulp initial pH 7.5, and 65°C for 30 min]. The lipase in question displayed a great potential for pitch control of TMP.
Keywords: Burkholderia sp. ZYB002; lipase; pitch control; production; thermomechanical pulping process (TMP)
Received: 2011-4-3
Accepted: 2011-8-17
Published Online: 2011-09-26
Published in Print: 2012-03-01
©2012 by Walter de Gruyter Berlin Boston
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Artikel in diesem Heft
- Original Papers
- Refiner bleaching in a peroxide-based ATMP process compared with conventional bleaching
- Chemical characterization of polymerized products formed in the reactions of matairesinol and pinoresinol with the stable radical 2,2-diphenyl-1-picrylhydrazyl
- Characterization of MWLs from Tamarix ramosissima isolated before and after hydrothermal treatment by spectroscopical and wet chemical methods
- Studies on electrooxidation of lignin and lignin model compounds. Part 1: Direct electrooxidation of non-phenolic lignin model compounds
- Studies on electro-oxidation of lignin and lignin model compounds. Part 2: N-Hydroxyphthalimide (NHPI)-mediated indirect electro-oxidation of non-phenolic lignin model compounds
- Shaped hemocompatible aerogels from cellulose phosphates: preparation and properties
- The effects of combining guaiacol and syringol on their pyrolysis
- Multi-steps degradation method for β-O-4 linkage in lignins: γ-TTSA method. Part 1: Reaction of non-phenolic dimeric β-O-4 model compounds
- Optimization of Burkholderia sp. ZYB002 lipase production for pitch control in thermomechanical pulping (TMP) processes
- Evidence that release of internal stress contributes to drying strains of wood
- Structure of oak wood from the Swedish warship Vasa revealed by X-ray scattering and microtomography
- Physical properties of simul (red-silk cotton) wood (Bombax ceiba L.) chemically modified with styrene acrylonitrile co-polymer and nanoclay
- Characterisation of moisture-dependent plasticity of beech wood and its application to a multi-surface plasticity model
- Vibration method for grading of large cross-section coniferous timber species
- Ultrastructural evaluation of compression wood-like properties of common juniper (Juniperus communis L.)
- Effect of ammonia addition to alkaline copper quaternary wood preservative solution on the distribution of copper complexes and leaching
- Morphological responses of Betula protoplasts in fiber spinning
- Erratum
- Surface chemistry of vessel elements by FE-SEM, μ-XPS and ToF-SIMS
- Meetings
- Meetings
- Masthead
- 10.1515/hf-2012-masthead3
Schlagwörter für diesen Artikel
Burkholderia sp. ZYB002;
lipase;
pitch control;
production;
thermomechanical pulping process (TMP)
Artikel in diesem Heft
- Original Papers
- Refiner bleaching in a peroxide-based ATMP process compared with conventional bleaching
- Chemical characterization of polymerized products formed in the reactions of matairesinol and pinoresinol with the stable radical 2,2-diphenyl-1-picrylhydrazyl
- Characterization of MWLs from Tamarix ramosissima isolated before and after hydrothermal treatment by spectroscopical and wet chemical methods
- Studies on electrooxidation of lignin and lignin model compounds. Part 1: Direct electrooxidation of non-phenolic lignin model compounds
- Studies on electro-oxidation of lignin and lignin model compounds. Part 2: N-Hydroxyphthalimide (NHPI)-mediated indirect electro-oxidation of non-phenolic lignin model compounds
- Shaped hemocompatible aerogels from cellulose phosphates: preparation and properties
- The effects of combining guaiacol and syringol on their pyrolysis
- Multi-steps degradation method for β-O-4 linkage in lignins: γ-TTSA method. Part 1: Reaction of non-phenolic dimeric β-O-4 model compounds
- Optimization of Burkholderia sp. ZYB002 lipase production for pitch control in thermomechanical pulping (TMP) processes
- Evidence that release of internal stress contributes to drying strains of wood
- Structure of oak wood from the Swedish warship Vasa revealed by X-ray scattering and microtomography
- Physical properties of simul (red-silk cotton) wood (Bombax ceiba L.) chemically modified with styrene acrylonitrile co-polymer and nanoclay
- Characterisation of moisture-dependent plasticity of beech wood and its application to a multi-surface plasticity model
- Vibration method for grading of large cross-section coniferous timber species
- Ultrastructural evaluation of compression wood-like properties of common juniper (Juniperus communis L.)
- Effect of ammonia addition to alkaline copper quaternary wood preservative solution on the distribution of copper complexes and leaching
- Morphological responses of Betula protoplasts in fiber spinning
- Erratum
- Surface chemistry of vessel elements by FE-SEM, μ-XPS and ToF-SIMS
- Meetings
- Meetings
- Masthead
- 10.1515/hf-2012-masthead3
