Quantification of fungal colonization in modified wood: Quantitative real-time PCR as a tool for studies on Trametes versicolor
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Annica Pilgård
Abstract
Traditional wood preservatives based on biocides are effective against wood-deteriorating organisms because of their toxicity. By contrast, modified woods are non-toxic by definition. To investigate the efficiency of various wood modifications, quantitative real-time polymerase chain reaction (qPCR) was used to profile the DNA amounts of the white-rot fungus Trametes versicolor (L.) [Lloyd strain CTB 863 A] during an 8-week-long growth period in treated Pinus sylvestris (L.) sapwood. The studied wood was modified by acetylation, furfurylation, and thermal treatment. The traditional wood preservatives bis-(N-cyclohexyldiazeniumdioxy)-copper (Cu-HDO) and chromated copper arsenate (CCA) were used as references, whereas untreated P. sylvestris (L.) sapwood served as a control. The maximum levels of fungal DNA in native wood occurred at the end of the experiment. For all wood treatments, the maximum fungal DNA level was recorded after an incubation period of 2 weeks, followed by a decline until the end of the trial. For the preservative-treated woods, Cu-HDO showed the lowest level of fungal DNA throughout the experiment, indicating that exploratory hyphal growth is limited owing to the phytotoxicity of the treatment. The other treatments did not inhibit the exploratory hyphal growth phase. We conclude that qPCR studies of hyphal growth patterns within wood should provide a powerful tool for evaluating and further optimizing new wood protection systems.
©2010 by Walter de Gruyter Berlin New York
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- Meetings
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Articles in the same Issue
- Original papers
- Side reactions of 4-acetamido-TEMPO as the catalyst in cellulose oxidation systems
- Reactions of aldehydes with chlorous acid and chlorite in chlorine dioxide bleaching
- Using cationic polyvinyl alcohol (C-PVA) to improve the strength of wood-free papers containing high-yield pulp (HYP)
- A new phenolic glucoside and flavonoids from the bark of Eucommia ulmoides Oliv.
- Decomposition of a phenolic lignin model compound over organic N-bases in an ionic liquid
- Investigation on wax-impregnated wood. Part 1: Microscopic observations and 2D X-ray imaging of distinct wax types
- Investigation on wax-impregnated wood. Part 2: Study of void spaces filled with air by He pycnometry, Hg intrusion porosimetry, and 3D X-ray imaging
- Surface properties of eucalyptus pulp fibres as reinforcement of cement-based composites
- Cure kinetics of PF/PVAc hybrid adhesive for manufacturing profiled wood-strand composites
- Moisture induced stresses perpendicular to the grain in glulam: Review and evaluation of the relative importance of models and parameters
- Three-dimensional modeling of the structure formation and consolidation of wood composites
- Observation of cell shapes in wood cross-sections during water adsorption by confocal laser-scanning microscopy (CLSM)
- A single cell model for pretreatment of wood by microwave explosion
- Pore space analysis of beech wood: The vessel network
- Quantification of fungal colonization in modified wood: Quantitative real-time PCR as a tool for studies on Trametes versicolor
- Copper monoethanolamine adsorption in wood and its relation with cation exchange capacity (CEC)
- Effect of amine ligand, copper/amine ratio, and pH on copper adsorption into wood
- Effect of different soils and pH amendments on brown-rot decay activity in a soil block test
- A proposed accelerated field stake test for rapid assessment of wood preservative systems
- Meetings
- Meetings
