Phylogenetic analysis of Aquilaria Lam. (Thymelaeaceae) based on DNA barcoding
-
Tingting Feng
,
Yesheng Wang
Abstract
Aquilaria Lam. is an important group of trees that produce agarwood, which is widely used for manufacturing medicine, perfumes and incense. The members of the genus Aquilaria are close to being extinct due to illegal harvesting and are now protected in many countries. In this study, five DNA barcodes [internal transcribed spacer (ITS), maturase K (matK), psbA-trnH, ribulose bisphosphate carboxylase (rbcL) and trnL-trnF] and their combinations were evaluated for the discrimination of the major (16 out of 21) species of Aquilaria based on three criteria: sequence variation, genetic distances and the discrimination ability. In addition, we attempted to determine the phylogenetic relationship between Aquilaria and Gyrinops species using three phylogenetic analysis methods. We observed that the combination barcode ITS+trnL-trnF had a good discrimination ability based on the best match and best close match methods, provided more genetic information, and clearly indicated the comprehensive phylogenetic relationship between most Aquilaria and Gyrinops species. Considering the overall performance of these barcodes, the ITS+trnL-trnF is a suitable barcode for the identification of Aquilaria species.
Funding source: National Natural Science Foundation of China
Award Identifier / Grant number: 81102418
Funding statement: This work was supported by the National Natural Science Foundation of China (Funder Id: 10.13039/501100001809, grant no. 81102418), Natural Science Foundation of Guangdong Province (Funder Id: 10.13039/501100003453, grant no. 2017A030313066), Guangdong Provincial Department of Science and Technology (grant no. 2015A030302087), State Key Laboratory of Applied Microbiology Southern China (grant no. SKLAM003-2015).
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Employment or leadership: None declared.
Honorarium: None declared.
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Supplementary Material
The online version of this article offers supplementary material (https://doi.org/10.1515/hf-2018-0127).
©2019 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Original Articles
- Phylogenetic analysis of Aquilaria Lam. (Thymelaeaceae) based on DNA barcoding
- The complete plastome of real yellow wood (Podocarpus latifolius): gene organization and comparison with related species
- Calibration of near infrared spectroscopy (NIRS) data of three Eucalyptus species with extractive contents determined by ASE extraction for rapid identification of species and high extractive contents
- Formation of heartwood, chemical composition of extractives and natural durability of plantation-grown teak wood from Mexico
- Investigations on densified beech wood for application as a swelling dowel in timber joints
- Variation of the contents of biphenyl structures in lignins among wood species
- Activation of glucose with Fenton’s reagent: chemical structures of activated products and their reaction efficacy toward cellulosic material
- Purification of pulp mill condensates by an adsorptive process on activated carbon
- Preparation of mineral bound particleboards with improved fire retardant and smoke suppression properties based on a mix of inorganic adhesive
- Influence of silane/MaPE dual coupling agents on the rheological and mechanical properties of sawdust/rubber/HDPE composites
Articles in the same Issue
- Frontmatter
- Original Articles
- Phylogenetic analysis of Aquilaria Lam. (Thymelaeaceae) based on DNA barcoding
- The complete plastome of real yellow wood (Podocarpus latifolius): gene organization and comparison with related species
- Calibration of near infrared spectroscopy (NIRS) data of three Eucalyptus species with extractive contents determined by ASE extraction for rapid identification of species and high extractive contents
- Formation of heartwood, chemical composition of extractives and natural durability of plantation-grown teak wood from Mexico
- Investigations on densified beech wood for application as a swelling dowel in timber joints
- Variation of the contents of biphenyl structures in lignins among wood species
- Activation of glucose with Fenton’s reagent: chemical structures of activated products and their reaction efficacy toward cellulosic material
- Purification of pulp mill condensates by an adsorptive process on activated carbon
- Preparation of mineral bound particleboards with improved fire retardant and smoke suppression properties based on a mix of inorganic adhesive
- Influence of silane/MaPE dual coupling agents on the rheological and mechanical properties of sawdust/rubber/HDPE composites
