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Accessing the natural variation of the abundances of major lignans in the heartwood of Taiwania cryptomerioides by 1H-NMR and LC-MS profiling

  • Nai-Wen Tsao , Shin-Hung Pan , Jeng-Der Chung , Yueh-Hsiung Kuo , Sheng-Yang Wang and Ying-Hsuan Sun EMAIL logo
Published/Copyright: January 7, 2019
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Holzforschung
From the journal Holzforschung Volume 73 Issue 5

Abstract

Lignans are major bioactive secondary metabolites, which are also formed in the heartwood (hW) of Taiwania (Taiwania cryptomerioides). Their biosynthesis pathways are complex and involve many enzymes and intermediates. To evaluate the extent of the genetic components leading to the variety of lignans in Taiwania hW, 35 Taiwania genotypes of four provenances were surveyed using the proton nuclear magnetic resonance (1H-NMR) and liquid chromatography-mass spectrometry (LC-MS) analyses. The metabolite profiles were statistically evaluated by principal component analysis (PCA) and the general linear model (GLM). The broad-sense heritability (H2) was further evaluated by linear mixed model (LMM) analysis. It was demonstrated that the genetic factor is the major contributor to the abundance of lignans, though the environmental factor also has some effect on it. Among the metabolites detected by 1H-NMR, lignans were the major compounds that exhibited high a H2 (0.52–0.82), which was further verified by LC-MS. The conclusion is that 1H-NMR spectroscopy is suitable for quick screenings, predictions and semi-quantitation of lignans. The high H2 is also indicative of the lignan abundances as traits that can be genetically modified to achieve a significant wood quality improvement.

Keywords: lH-NMR; broad-sense heritability; heartwood; LC-MS; lignan; metabolomics analysis; natural variation; Taiwania cryptomerioides

Acknowledgments

We thank Tungshih District office, Forestry Bureau of Taiwan, for their assistance in sample collection. We thank Mrs. Chiu-Yueh Hung for her assistance in preparing the manuscript.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: This project was supported by the Ministry of Science and Technology, Taiwan, (MOST 104-2321-B-005-010 and 104-2321-B-005-011) and the Ministry of Education, Taiwan, R.O.C under the ATU plan.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

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Received: 2018-05-22
Accepted: 2018-11-21
Published Online: 2019-01-07
Published in Print: 2019-05-27

©2019 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Frontmatter
  2. Original Articles
  3. Accessing the natural variation of the abundances of major lignans in the heartwood of Taiwania cryptomerioides by 1H-NMR and LC-MS profiling
  4. Rapid detection of several endangered agarwood-producing Aquilaria species and their potential adulterants using plant DNA barcodes coupled with high-resolution melting (Bar-HRM) analysis
  5. Changes in sorption and electrical properties of wood caused by fungal decay
  6. Effect of hardening parameters of wood preservatives based on tannin copolymers
  7. In situ polymerization of 2-hydroxyethyl methacrylate (HEMA) and 3-(methacryloxy)propyltrimethoxysilane (MAPTES) in poplar cell wall to enhance its dimensional stability
  8. Isolation of pure pinosylvins from industrial knotwood residue with non-chlorinated solvents
  9. Towards better UV-blocking and antioxidant performance of varnish via additives based on lignin and its colloids
  10. Thermal stability of lignin in ground pulp (GP) and the effect of lignin modification on GP’s thermal stability: TGA experiments with dimeric lignin model compounds and milled wood lignins
  11. Preparation, structural changes and adsorption performance of heavy metal ions on sulfonated cellulose with varying degrees of substitution
  12. Mass transport and yield during spinning of lignin-cellulose carbon fiber precursors
https://doi.org/10.1515/hf-2018-0117
Keywords for this article
lH-NMR; broad-sense heritability; heartwood; LC-MS; lignan; metabolomics analysis; natural variation; Taiwania cryptomerioides

Articles in the same Issue

  1. Frontmatter
  2. Original Articles
  3. Accessing the natural variation of the abundances of major lignans in the heartwood of Taiwania cryptomerioides by 1H-NMR and LC-MS profiling
  4. Rapid detection of several endangered agarwood-producing Aquilaria species and their potential adulterants using plant DNA barcodes coupled with high-resolution melting (Bar-HRM) analysis
  5. Changes in sorption and electrical properties of wood caused by fungal decay
  6. Effect of hardening parameters of wood preservatives based on tannin copolymers
  7. In situ polymerization of 2-hydroxyethyl methacrylate (HEMA) and 3-(methacryloxy)propyltrimethoxysilane (MAPTES) in poplar cell wall to enhance its dimensional stability
  8. Isolation of pure pinosylvins from industrial knotwood residue with non-chlorinated solvents
  9. Towards better UV-blocking and antioxidant performance of varnish via additives based on lignin and its colloids
  10. Thermal stability of lignin in ground pulp (GP) and the effect of lignin modification on GP’s thermal stability: TGA experiments with dimeric lignin model compounds and milled wood lignins
  11. Preparation, structural changes and adsorption performance of heavy metal ions on sulfonated cellulose with varying degrees of substitution
  12. Mass transport and yield during spinning of lignin-cellulose carbon fiber precursors
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